Restriction map for pF1KE1043
 (Linear) (Six-base) MAPSORT of: KIEE1043.seq  Check: 6648  from: 1  to: 1410



KIEE1043.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     106    1029    1410 
   Size:        106     923     381

  Fragments arranged by size: 

                 923     381     106
 
AccI GT'mk_AC

Cuts at:      0     494    1410 
   Size:        494     916
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     426     736     981    1410 
   Size:        426     310     245     429

  Fragments arranged by size: 

                 429     426     310     245
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1061    1410 
   Size:       1061     349
 
AlwNI CAG_nnn'CTG

Cuts at:      0     873    1410 
   Size:        873     537
 
ApoI r'AATT_y

Cuts at:      0    1245    1410 
   Size:       1245     165
 
AvaI C'yCGr_G

Cuts at:      0     692    1410 
   Size:        692     718
 
BanI G'GyrC_C

Cuts at:      0     410     514     779    1114    1410 
   Size:        410     104     265     335     296

  Fragments arranged by size: 

                 410     335     296     265     104
 
BanII G_rGCy'C

Cuts at:      0    1276    1410 
   Size:       1276     134
 
BbeI G_GCGC'C

Cuts at:      0     414    1410 
   Size:        414     996
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     144    1359    1410 
   Size:        144    1215      51

  Fragments arranged by size: 

                1215     144      51
 
BbvCI CC'TCA_GC

Cuts at:      0    1405    1410 
   Size:       1405       5
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     865     899    1410 
   Size:        865      34     511

  Fragments arranged by size: 

                 865     511      34
 
BglII A'GATC_T

Cuts at:      0     194    1410 
   Size:        194    1216
 
BlpI GC'TnA_GC

Cuts at:      0     361    1410 
   Size:        361    1049
 
Bme1580I G_kGCm'C

Cuts at:      0      55     163     481     519     565     643     685     784 
   Size:         55     108     318      38      46      78      42      99

Cuts at:    784     849    1081    1410 
   Size:         65     232     329

  Fragments arranged by size: 

                 329     318     232     108      99      78      65      55
                  46      42      38
 
BmgBI CAC'GTC

Cuts at:      0    1209    1410 
   Size:       1209     201
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     294     916    1410 
   Size:         46     248     622     494

  Fragments arranged by size: 

                 622     494     248      46
 
Bpu10I CC'TnA_GC

Cuts at:      0     785    1405    1410 
   Size:        785     620       5

  Fragments arranged by size: 

                 785     620       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400    1009    1153    1410 
   Size:        400     609     144     257

  Fragments arranged by size: 

                 609     400     257     144
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1210    1410 
   Size:       1210     200
 
BsaAI yAC'GTr

Cuts at:      0    1265    1410 
   Size:       1265     145
 
BsaHI Gr'CG_yC

Cuts at:      0     411    1410 
   Size:        411     999
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274     826     913    1410 
   Size:        274     552      87     497

  Fragments arranged by size: 

                 552     497     274      87
 
BseYI C'CCAG_C

Cuts at:      0     542    1002    1410 
   Size:        542     460     408

  Fragments arranged by size: 

                 542     460     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     365     567     576    1410 
   Size:        106     259     202       9     834

  Fragments arranged by size: 

                 834     259     202     106       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     746     929    1410 
   Size:        746     183     481

  Fragments arranged by size: 

                 746     481     183
 
BsmI GAATG_Cn'

Cuts at:      0    1048    1410 
   Size:       1048     362
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     163     481     519     565     643     685     746 
   Size:         55     108     318      38      46      78      42      61

Cuts at:    746     784     849     929    1081    1276    1410 
   Size:         38      65      80     152     195     134

  Fragments arranged by size: 

                 318     195     152     134     108      80      78      65
                  61      55      46      42      38      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78     106     130     322     387     710    1029    1410 
   Size:         78      28      24     192      65     323     319     381

  Fragments arranged by size: 

                 381     323     319     192      78      65      28      24
            
 
BsrDI GCAATG_nn'

Cuts at:      0     800    1068    1410 
   Size:        800     268     342

  Fragments arranged by size: 

                 800     342     268
 
BsrFI r'CCGG_y

Cuts at:      0     857    1410 
   Size:        857     553
 
BsrGI T'GTAC_A

Cuts at:      0    1094    1410 
   Size:       1094     316
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     590     873    1410 
   Size:        590     283     537

  Fragments arranged by size: 

                 590     537     283
 
BstBI TT'CG_AA

Cuts at:      0    1335    1410 
   Size:       1335      75
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1265    1410 
   Size:       1265     145
 
BstYI r'GATC_y

Cuts at:      0     194    1400    1410 
   Size:        194    1206      10

  Fragments arranged by size: 

                1206     194      10
 
Bsu36I CC'TnA_GG

Cuts at:      0     164    1410 
   Size:        164    1246
 
BtsI GCAGTG_nn'

Cuts at:      0     921    1074    1410 
   Size:        921     153     336

  Fragments arranged by size: 

                 921     336     153
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     654     689    1050    1085    1410 
   Size:        654      35     361      35     325

  Fragments arranged by size: 

                 654     361     325      35      35
 
EaeI y'GGCC_r

Cuts at:      0     102     833    1410 
   Size:        102     731     577

  Fragments arranged by size: 

                 731     577     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     294     426     736     916     981    1410 
   Size:         46     248     132     310     180      65     429

  Fragments arranged by size: 

                 429     310     248     180     132      65      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     699    1410 
   Size:        699     711
 
HaeII r_GCGC'y

Cuts at:      0     414    1410 
   Size:        414     996
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     889     921    1410 
   Size:        889      32     489

  Fragments arranged by size: 

                 889     489      32
 
KasI G'GCGC_C

Cuts at:      0     410    1410 
   Size:        410    1000
 
MscI TGG'CCA

Cuts at:      0     835    1410 
   Size:        835     575
 
MslI CAynn'nnrTG

Cuts at:      0    1022    1102    1410 
   Size:       1022      80     308

  Fragments arranged by size: 

                1022     308      80
 
MspA1I CmG'CkG

Cuts at:      0     245     272     725    1410 
   Size:        245      27     453     685

  Fragments arranged by size: 

                 685     453     245      27
 
NaeI GCC'GGC

Cuts at:      0     859    1410 
   Size:        859     551
 
NarI GG'CG_CC

Cuts at:      0     411    1410 
   Size:        411     999
 
NgoMIV G'CCGG_C

Cuts at:      0     857    1410 
   Size:        857     553
 
NspI r_CATG'y

Cuts at:      0     550     808    1410 
   Size:        550     258     602

  Fragments arranged by size: 

                 602     550     258
 
PasI CC'CwG_GG

Cuts at:      0    1186    1410 
   Size:       1186     224
 
PfoI T'CCnGG_A

Cuts at:      0      23    1410 
   Size:         23    1387
 
PmlI CAC'GTG

Cuts at:      0    1265    1410 
   Size:       1265     145
 
PpuMI rG'GwC_Cy

Cuts at:      0     699    1410 
   Size:        699     711
 
PstI C_TGCA'G

Cuts at:      0     131     319     878    1040    1410 
   Size:        131     188     559     162     370

  Fragments arranged by size: 

                 559     370     188     162     131
 
PvuII CAG'CTG

Cuts at:      0     725    1410 
   Size:        725     685
 
SbfI CC_TGCA'GG

Cuts at:      0     131    1410 
   Size:        131    1279
 
SfcI C'TryA_G

Cuts at:      0     127     295     315     874     961    1036    1410 
   Size:        127     168      20     559      87      75     374

  Fragments arranged by size: 

                 559     374     168     127      87      75      20
 
SfoI GGC'GCC

Cuts at:      0     412    1410 
   Size:        412     998
 
SmlI C'TyrA_G

Cuts at:      0     415     988    1132    1410 
   Size:        415     573     144     278

  Fragments arranged by size: 

                 573     415     278     144
 
SphI G_CATG'C

Cuts at:      0     550    1410 
   Size:        550     860
 
StuI AGG'CCT

Cuts at:      0     454    1410 
   Size:        454     956
 
StyI C'CwwG_G

Cuts at:      0     643     971    1410 
   Size:        643     328     439

  Fragments arranged by size: 

                 643     439     328
 
TatI w'GTAC_w

Cuts at:      0     219    1094    1410 
   Size:        219     875     316

  Fragments arranged by size: 

                 875     316     219
 
XmnI GAAnn'nnTTC

Cuts at:      0    1246    1410 
   Size:       1246     164
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI    AlwNI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BglII     BlpI Bme1580I
    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI
    BsrGI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtsI    CspCI
     EaeI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PfoI     PmlI
    PpuMI     PstI    PvuII     SbfI     SfcI     SfoI     SmlI     SphI
     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI     BclI    BfrBI     BglI     BmrI     BmtI
     BplI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI   BssHII    BssSI   BstEII  BstZ17I     BtgI    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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