Restriction map for pF1KE1036
 (Linear) (Six-base) MAPSORT of: KIEE1036.seq  Check: 5568  from: 1  to: 1674



KIEE1036.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     313    1674 
   Size:        313    1361
 
AccI GT'mk_AC

Cuts at:      0     473    1674 
   Size:        473    1201
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     401    1372    1674 
   Size:        176     225     971     302

  Fragments arranged by size: 

                 971     302     225     176
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1323    1674 
   Size:       1323     351
 
AleI CACnn'nnGTG

Cuts at:      0     958    1674 
   Size:        958     716
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     976    1674 
   Size:        976     698
 
AlwNI CAG_nnn'CTG

Cuts at:      0     109     128     673     840    1674 
   Size:        109      19     545     167     834

  Fragments arranged by size: 

                 834     545     167     109      19
 
ApaI G_GGCC'C

Cuts at:      0      75     523    1526    1674 
   Size:         75     448    1003     148

  Fragments arranged by size: 

                1003     448     148      75
 
AvaI C'yCGr_G

Cuts at:      0     512    1376    1674 
   Size:        512     864     298

  Fragments arranged by size: 

                 864     512     298
 
AvrII C'CTAG_G

Cuts at:      0      24     645    1674 
   Size:         24     621    1029

  Fragments arranged by size: 

                1029     621      24
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1242    1275    1674 
   Size:       1242      33     399

  Fragments arranged by size: 

                1242     399      33
 
BanI G'GyrC_C

Cuts at:      0     279     650     749     960    1149    1667    1674 
   Size:        279     371      99     211     189     518       7

  Fragments arranged by size: 

                 518     371     279     211     189      99       7
 
BanII G_rGCy'C

Cuts at:      0      75     523     560     827    1526    1674 
   Size:         75     448      37     267     699     148

  Fragments arranged by size: 

                 699     448     267     148      75      37
 
BbeI G_GCGC'C

Cuts at:      0     654     753    1674 
   Size:        654      99     921

  Fragments arranged by size: 

                 921     654      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     407    1390    1674 
   Size:        407     983     284

  Fragments arranged by size: 

                 983     407     284
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     126     674    1382    1674 
   Size:        126     548     708     292

  Fragments arranged by size: 

                 708     548     292     126
 
BglI GCCn_nnn'nGGC

Cuts at:      0     341    1087    1674 
   Size:        341     746     587

  Fragments arranged by size: 

                 746     587     341
 
BlpI GC'TnA_GC

Cuts at:      0      13     821    1674 
   Size:         13     808     853

  Fragments arranged by size: 

                 853     808      13
 
Bme1580I G_kGCm'C

Cuts at:      0      75     156     523    1154    1526    1674 
   Size:         75      81     367     631     372     148

  Fragments arranged by size: 

                 631     372     367     148      81      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     360     492    1674 
   Size:        360     132    1182

  Fragments arranged by size: 

                1182     360     132
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      78    1446    1464    1674 
   Size:         78    1368      18     210

  Fragments arranged by size: 

                1368     210      78      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178    1203    1459    1557    1674 
   Size:        178    1025     256      98     117

  Fragments arranged by size: 

                1025     256     178     117      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282    1674 
   Size:        282    1392
 
BsaAI yAC'GTr

Cuts at:      0     137    1085    1674 
   Size:        137     948     589

  Fragments arranged by size: 

                 948     589     137
 
BsaHI Gr'CG_yC

Cuts at:      0      94     651     750    1674 
   Size:         94     557      99     924

  Fragments arranged by size: 

                 924     557      99      94
 
BsaWI w'CCGG_w

Cuts at:      0     458    1274    1674 
   Size:        458     816     400

  Fragments arranged by size: 

                 816     458     400
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1228    1258    1674 
   Size:       1228      30     416

  Fragments arranged by size: 

                1228     416      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     210     577    1405    1419    1674 
   Size:        210     367     828      14     255

  Fragments arranged by size: 

                 828     367     255     210      14
 
BseYI C'CCAG_C

Cuts at:      0     298     493     865    1641    1674 
   Size:        298     195     372     776      33

  Fragments arranged by size: 

                 776     372     298     195      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1266    1580    1591    1614    1674 
   Size:        547     719     314      11      23      60

  Fragments arranged by size: 

                 719     547     314      60      23      11
 
BsmI GAATG_Cn'

Cuts at:      0     867    1674 
   Size:        867     807
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      85     894    1674 
   Size:         85     809     780

  Fragments arranged by size: 

                 809     780      85
 
Bsp1286I G_dGCh'C

Cuts at:      0      75     156     523     560     827    1154    1526    1674 
   Size:         75      81     367      37     267     327     372     148

  Fragments arranged by size: 

                 372     367     327     267     148      81      75      37
            
 
BspEI T'CCGG_A

Cuts at:      0     458    1274    1674 
   Size:        458     816     400

  Fragments arranged by size: 

                 816     458     400
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     313     689    1674 
   Size:        313     376     985

  Fragments arranged by size: 

                 985     376     313
 
BsrBI CCG'CTC

Cuts at:      0    1502    1674 
   Size:       1502     172
 
BsrDI GCAATG_nn'

Cuts at:      0     304     786    1344    1674 
   Size:        304     482     558     330

  Fragments arranged by size: 

                 558     482     330     304
 
BsrFI r'CCGG_y

Cuts at:      0     371     806    1674 
   Size:        371     435     868

  Fragments arranged by size: 

                 868     435     371
 
BsrGI T'GTAC_A

Cuts at:      0     245    1674 
   Size:        245    1429
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     109    1674 
   Size:        109    1565
 
BstEII G'GTnAC_C

Cuts at:      0    1194    1674 
   Size:       1194     480
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     630     801     900     996    1125    1181    1674 
   Size:        630     171      99      96     129      56     493

  Fragments arranged by size: 

                 630     493     171     129      99      96      56
 
Bsu36I CC'TnA_GG

Cuts at:      0    1313    1674 
   Size:       1313     361
 
BtgI C'CryG_G

Cuts at:      0     989    1304    1674 
   Size:        989     315     370

  Fragments arranged by size: 

                 989     370     315
 
BtsI GCAGTG_nn'

Cuts at:      0     107    1076    1420    1626    1674 
   Size:        107     969     344     206      48

  Fragments arranged by size: 

                 969     344     206     107      48
 
EaeI y'GGCC_r

Cuts at:      0      77     348     466    1674 
   Size:         77     271     118    1208

  Fragments arranged by size: 

                1208     271     118      77
 
EarI CTCTTCn'nnn_

Cuts at:      0     731    1469    1674 
   Size:        731     738     205

  Fragments arranged by size: 

                 738     731     205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     178     401    1203    1372    1459    1557    1674 
   Size:        176       2     223     802     169      87      98     117

  Fragments arranged by size: 

                 802     223     176     169     117      98      87       2
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      28      71      72     519     520     693    1188    1258 
   Size:         28      43       1     447       1     173     495      70

Cuts at:   1258    1674 
   Size:        416

  Fragments arranged by size: 

                 495     447     416     173      70      43      28       1
                   1
 
HaeII r_GCGC'y

Cuts at:      0     654     753    1674 
   Size:        654      99     921

  Fragments arranged by size: 

                 921     654      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     648     680     902     934    1200    1232    1309    1341 
   Size:        648      32     222      32     266      32      77      32

Cuts at:   1341    1674 
   Size:        333

  Fragments arranged by size: 

                 648     333     266     222      77      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     474    1674 
   Size:        474    1200
 
KasI G'GCGC_C

Cuts at:      0     650     749    1674 
   Size:        650      99     925

  Fragments arranged by size: 

                 925     650      99
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1352    1400    1674 
   Size:       1352      48     274

  Fragments arranged by size: 

                1352     274      48
 
MscI TGG'CCA

Cuts at:      0     468    1674 
   Size:        468    1206
 
MslI CAynn'nnrTG

Cuts at:      0     703     799     958     994    1674 
   Size:        703      96     159      36     680

  Fragments arranged by size: 

                 703     680     159      96      36
 
MspA1I CmG'CkG

Cuts at:      0     353     820    1181    1242    1437    1578    1674 
   Size:        353     467     361      61     195     141      96

  Fragments arranged by size: 

                 467     361     353     195     141      96      61
 
NarI GG'CG_CC

Cuts at:      0     651     750    1674 
   Size:        651      99     924

  Fragments arranged by size: 

                 924     651      99
 
NcoI C'CATG_G

Cuts at:      0     989    1674 
   Size:        989     685
 
NspI r_CATG'y

Cuts at:      0     169    1674 
   Size:        169    1505
 
PasI CC'CwG_GG

Cuts at:      0     828    1674 
   Size:        828     846
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     128    1023    1674 
   Size:        128     895     651

  Fragments arranged by size: 

                 895     651     128
 
PfoI T'CCnGG_A

Cuts at:      0    1124    1674 
   Size:       1124     550
 
PmlI CAC'GTG

Cuts at:      0    1085    1674 
   Size:       1085     589
 
PpuMI rG'GwC_Cy

Cuts at:      0    1258    1674 
   Size:       1258     416
 
PshAI GACnn'nnGTC

Cuts at:      0    1484    1674 
   Size:       1484     190
 
PspOMI G'GGCC_C

Cuts at:      0      71     519    1522    1674 
   Size:         71     448    1003     152

  Fragments arranged by size: 

                1003     448     152      71
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     361     393    1674 
   Size:        361      32    1281

  Fragments arranged by size: 

                1281     361      32
 
PstI C_TGCA'G

Cuts at:      0     689     716    1364    1674 
   Size:        689      27     648     310

  Fragments arranged by size: 

                 689     648     310      27
 
PvuII CAG'CTG

Cuts at:      0     820    1181    1242    1437    1578    1674 
   Size:        820     361      61     195     141      96

  Fragments arranged by size: 

                 820     361     195     141      96      61
 
SalI G'TCGA_C

Cuts at:      0     472    1674 
   Size:        472    1202
 
SexAI A'CCwGG_T

Cuts at:      0     629    1145    1674 
   Size:        629     516     529

  Fragments arranged by size: 

                 629     529     516
 
SfcI C'TryA_G

Cuts at:      0     685     712    1360    1674 
   Size:        685      27     648     314

  Fragments arranged by size: 

                 685     648     314      27
 
SfoI GGC'GCC

Cuts at:      0     652     751    1674 
   Size:        652      99     923

  Fragments arranged by size: 

                 923     652      99
 
SmaI CCC'GGG

Cuts at:      0    1378    1674 
   Size:       1378     296
 
SmlI C'TyrA_G

Cuts at:      0     261     512    1674 
   Size:        261     251    1162

  Fragments arranged by size: 

                1162     261     251
 
SnaBI TAC'GTA

Cuts at:      0     137    1674 
   Size:        137    1537
 
StuI AGG'CCT

Cuts at:      0    1426    1674 
   Size:       1426     248
 
StyI C'CwwG_G

Cuts at:      0      24     645     989    1154    1283    1674 
   Size:         24     621     344     165     129     391

  Fragments arranged by size: 

                 621     391     344     165     129      24
 
TatI w'GTAC_w

Cuts at:      0     245    1263    1674 
   Size:        245    1018     411

  Fragments arranged by size: 

                1018     411     245
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1228    1260    1293    1325    1674 
   Size:       1228      32      33      32     349

  Fragments arranged by size: 

                1228     349      33      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     630     996    1020    1674 
   Size:        630     366      24     654

  Fragments arranged by size: 

                 654     630     366      24
 
XhoI C'TCGA_G

Cuts at:      0     512    1674 
   Size:        512    1162
 
XmaI C'CCGG_G

Cuts at:      0    1376    1674 
   Size:       1376     298
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AleI     AlfI    AlwNI     ApaI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BciVI
     BglI     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
   BstEII    BstXI   Bsu36I     BtgI     BtsI     EaeI     EarI  Eco57MI
 EcoO109I    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NspI     PasI    PflMI     PfoI     PmlI
    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII     SalI    SexAI
     SfcI     SfoI     SmaI     SmlI    SnaBI     StuI     StyI     TatI
     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    BamHI    BbvCI     BcgI
     BclI    BfrBI    BglII    BmgBI     BmtI   Bpu10I     BsaI    BsaBI
    BsiEI  BsiHKAI    BsiWI    BspHI   BssHII    BssSI    BstBI    BstYI
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PpiI     PsiI    PspXI     PvuI    RsrII     SacI    SacII    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XmnI     ZraI
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