Restriction map for pF1KE1034
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BfrI        BglII       
BlnI        BpmI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstYI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MamI        MfeI        MflI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   25 enzymes) #
###################################

Alw44I        121  GTGCAC               G^TGCAC
ApaLI         121  GTGCAC               G^TGCAC
AspHI         121  GTGCAC               G[AT]GC[AT]^C
BanII         635  GAGCCC               G[GA]GC[CT]^C
BfaI          824  CTAG                 C^TAG
BlpI          565  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      565  GCTGAGC              GC^T[ACGT]AGC
BseRI         711  GAGGAG               GAGGAG(10/8)
BsiHKAI       121  GTGCAC               G[AT]GC[AT]^C
BsmAI         334  GAGAC                GTCTC(1/5)
BspHI         203  TCATGA               T^CATGA
CelII         565  GCTGAGC              GC^T[ACGT]AGC
DpnII        1029  GATC                 ^GATC
DrdI          253  GACATCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII        1184  AGCGCC               [GA]GCGC^[CT]
MaeI          824  CTAG                 C^TAG
MboI         1029  GATC                 ^GATC
NcoI          758  CCATGG               C^CATGG
NdeII        1029  GATC                 ^GATC
RcaI          203  TCATGA               T^CATGA
Sau3AI       1029  GATC                 ^GATC
SmlI          217  CTCAAG               C^T[CT][GA]AG
SspI          487  AATATT               AAT^ATT
TaqI         1025  TCGA                 T^CGA
TthHB8I      1025  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI           4  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              313  CAGTTTCTG           
BalI          389  TGGCCA               TGG^CCA
              770  TGGCCA              
BciVI         597  GGATAC               GTATCC(6/5)
              740  GTATCC              
BsgI         1140  CTGCAC               GTGCAG(16/14)
             1112  GTGCAG              
BsiEI          41  CGGCCG               CG[GA][CT]^CG
              101  CGGCCG              
BsrBI          81  GAGCGG               CCGCTC(-3/-3)
              568  GAGCGG              
BstAPI          3  GCAGAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
               12  GCAGGTAGTGC         
BstXI         455  CCATGTCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              851  CCATCCTGCTGG        
EagI           41  CGGCCG               C^GGCCG
              101  CGGCCG              
EarI          880  GAAGAG               CTCTTC(1/4)
             1194  GAAGAG              
EclXI          41  CGGCCG               C^GGCCG
              101  CGGCCG              
Eco52I         41  CGGCCG               C^GGCCG
              101  CGGCCG              
FauI           90  CCCGC                CCCGC(4/6)
              151  GCGGG               
HphI          831  GGTGA                GGTGA(8/7)
             1095  GGTGA               
Ksp632I       880  GAAGAG               CTCTTC(1/4)
             1194  GAAGAG              
MluNI         389  TGGCCA               TGG^CCA
              770  TGGCCA              
MscI          389  TGGCCA               TGG^CCA
              770  TGGCCA              
PleI         1079  GACTC                GAGTC(4/5)
              451  GAGTC               
PpuMI         905  GGGTCCT              [GA]G^G[AT]CC[CT]
              932  GGGTCCC             
PstI           10  CTGCAG               CTGCA^G
              274  CTGCAG              
StuI           95  AGGCCT               AGG^CCT
             1037  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AluI        AocI        AvaII       
BanI        BbvI        BcnI        BglI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DraII       DsaI        EaeI        Eco81I      
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        KspI        MaeII       MaeIII      
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       PvuII       SacII       Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        TseI        
Tsp45I      TspRI       XcmI        
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