Restriction map for pF1KE1034
 (Linear) (Six-base) MAPSORT of: KIEE1034.seq  Check: 5664  from: 1  to: 1272



KIEE1034.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     391    1272 
   Size:        391     881
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1201    1272 
   Size:        799     402      71

  Fragments arranged by size: 

                 799     402      71
 
AlwNI CAG_nnn'CTG

Cuts at:      0       9     318    1272 
   Size:          9     309     954

  Fragments arranged by size: 

                 954     309       9
 
ApaLI G'TGCA_C

Cuts at:      0     121    1272 
   Size:        121    1151
 
BanI G'GyrC_C

Cuts at:      0      85     703    1127    1272 
   Size:         85     618     424     145

  Fragments arranged by size: 

                 618     424     145      85
 
BanII G_rGCy'C

Cuts at:      0     639    1272 
   Size:        639     633
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     591     751    1272 
   Size:        591     160     521

  Fragments arranged by size: 

                 591     521     160
 
BglI GCCn_nnn'nGGC

Cuts at:      0      94     152     161    1272 
   Size:         94      58       9    1111

  Fragments arranged by size: 

                1111      94      58       9
 
BlpI GC'TnA_GC

Cuts at:      0     566    1272 
   Size:        566     706
 
Bme1580I G_kGCm'C

Cuts at:      0      90     125     706    1130    1152    1272 
   Size:         90      35     581     424      22     120

  Fragments arranged by size: 

                 581     424     120      90      35      22
 
BmgBI CAC'GTC

Cuts at:      0     111     225    1014    1272 
   Size:        111     114     789     258

  Fragments arranged by size: 

                 789     258     114     111
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     306     970    1144    1154    1272 
   Size:        306     664     174      10     118

  Fragments arranged by size: 

                 664     306     174     118      10
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     210    1272 
   Size:        210    1062
 
Bpu10I CC'TnA_GC

Cuts at:      0      79    1272 
   Size:         79    1193
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1272 
   Size:        202    1070
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     442     472    1272 
   Size:        442      30     800

  Fragments arranged by size: 

                 800     442      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     726    1272 
   Size:        726     546
 
BseYI C'CCAG_C

Cuts at:      0      21     246     617     748    1272 
   Size:         21     225     371     131     524

  Fragments arranged by size: 

                 524     371     225     131      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1125    1133    1272 
   Size:       1125       8     139

  Fragments arranged by size: 

                1125     139       8
 
BsiEI CG_ry'CG

Cuts at:      0      44     104    1272 
   Size:         44      60    1168

  Fragments arranged by size: 

                1168      60      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     125    1272 
   Size:        125    1147
 
Bsp1286I G_dGCh'C

Cuts at:      0      90     125     639     706    1130    1152    1272 
   Size:         90      35     514      67     424      22     120

  Fragments arranged by size: 

                 514     424     120      90      67      35      22
 
BspHI T'CATG_A

Cuts at:      0     203    1272 
   Size:        203    1069
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       3     391     969    1272 
   Size:          3     388     578     303

  Fragments arranged by size: 

                 578     388     303       3
 
BsrBI CCG'CTC

Cuts at:      0      83     570    1272 
   Size:         83     487     702

  Fragments arranged by size: 

                 702     487      83
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0       9      18    1272 
   Size:          9       9    1254

  Fragments arranged by size: 

                1254       9       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     462     858    1272 
   Size:        462     396     414

  Fragments arranged by size: 

                 462     414     396
 
Bsu36I CC'TnA_GG

Cuts at:      0      31     910    1122    1272 
   Size:         31     879     212     150

  Fragments arranged by size: 

                 879     212     150      31
 
BtgI C'CryG_G

Cuts at:      0     104     156     209     683     758    1188    1272 
   Size:        104      52      53     474      75     430      84

  Fragments arranged by size: 

                 474     430     104      84      75      53      52
 
BtsI GCAGTG_nn'

Cuts at:      0     392     929    1121    1272 
   Size:        392     537     192     151

  Fragments arranged by size: 

                 537     392     192     151
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     259    1272 
   Size:        259    1013
 
EaeI y'GGCC_r

Cuts at:      0      41     101     137     389     770    1272 
   Size:         41      60      36     252     381     502

  Fragments arranged by size: 

                 502     381     252      60      41      36
 
EagI C'GGCC_G

Cuts at:      0      41     101    1272 
   Size:         41      60    1171

  Fragments arranged by size: 

                1171      60      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     875    1189    1272 
   Size:        875     314      83

  Fragments arranged by size: 

                 875     314      83
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     733    1272 
   Size:        733     539
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1201    1272 
   Size:        799     402      71

  Fragments arranged by size: 

                 799     402      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     765     906     933    1272 
   Size:        765     141      27     339

  Fragments arranged by size: 

                 765     339     141      27
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     804    1272 
   Size:        804     468
 
HaeII r_GCGC'y

Cuts at:      0    1188    1272 
   Size:       1188      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     442     474    1145    1177    1272 
   Size:        442      32     671      32      95

  Fragments arranged by size: 

                 671     442      95      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     243    1272 
   Size:        243    1029
 
MscI TGG'CCA

Cuts at:      0     391     772    1272 
   Size:        391     381     500

  Fragments arranged by size: 

                 500     391     381
 
MslI CAynn'nnrTG

Cuts at:      0     433     721     757    1272 
   Size:        433     288      36     515

  Fragments arranged by size: 

                 515     433     288      36
 
MspA1I CmG'CkG

Cuts at:      0     106     158     211     246     814     847    1246    1272 
   Size:        106      52      53      35     568      33     399      26

  Fragments arranged by size: 

                 568     399     106      53      52      35      33      26
            
 
NcoI C'CATG_G

Cuts at:      0     758    1272 
   Size:        758     514
 
PasI CC'CwG_GG

Cuts at:      0    1104    1264    1272 
   Size:       1104     160       8

  Fragments arranged by size: 

                1104     160       8
 
PfoI T'CCnGG_A

Cuts at:      0     329    1272 
   Size:        329     943
 
PpuMI rG'GwC_Cy

Cuts at:      0     906     933    1272 
   Size:        906      27     339

  Fragments arranged by size: 

                 906     339      27
 
PstI C_TGCA'G

Cuts at:      0      14     278    1272 
   Size:         14     264     994

  Fragments arranged by size: 

                 994     264      14
 
PvuII CAG'CTG

Cuts at:      0     246     814     847    1272 
   Size:        246     568      33     425

  Fragments arranged by size: 

                 568     425     246      33
 
SacII CC_GC'GG

Cuts at:      0     107     159     212    1272 
   Size:        107      52      53    1060

  Fragments arranged by size: 

                1060     107      53      52
 
SanDI GG'GwC_CC

Cuts at:      0     933    1272 
   Size:        933     339
 
SfcI C'TryA_G

Cuts at:      0      10     274     379    1272 
   Size:         10     264     105     893

  Fragments arranged by size: 

                 893     264     105      10
 
SmlI C'TyrA_G

Cuts at:      0     217    1272 
   Size:        217    1055
 
SspI AAT'ATT

Cuts at:      0     489    1272 
   Size:        489     783
 
StuI AGG'CCT

Cuts at:      0      97    1039    1272 
   Size:         97     942     233

  Fragments arranged by size: 

                 942     233      97
 
StyI C'CwwG_G

Cuts at:      0     758     872     900    1272 
   Size:        758     114      28     372

  Fragments arranged by size: 

                 758     372     114      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1145    1272 
   Size:       1145     127
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     756     765     835    1272 
   Size:        756       9      70     437

  Fragments arranged by size: 

                 756     437      70       9
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI    ApaLI     BanI    BanII    BciVI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI   BstAPI    BstXI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NcoI     PasI     PfoI    PpuMI
     PstI    PvuII    SacII    SanDI     SfcI     SmlI     SspI     StuI
     StyI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI    BglII     BmtI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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