Restriction map for pF1KB8505
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AgeI        Aor51HI     AscI        Asp718I     AspI        
AvaI        AviII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BglII       BsaAI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       FauI        
FseI        FspI        HaeII       HhaI        HinP1I      
KasI        KpnI        KspI        MluI        MroI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AciI         2490  CCGC                 CCGC(-3/-1)
AcyI         2418  GGCGTC               G[GA]^CG[CT]C
AflII        1806  CTTAAG               C^TTAAG
AflIII       1975  ACATGT               A^C[GA][CT]GT
AhdI          582  GACTTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2060  GTGCAC               G^TGCAC
AlwNI        1373  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          424  CCTGAGG              CC^T[ACGT]AGG
ApaI         2709  GGGCCC               GGGCC^C
ApaLI        2060  GTGCAC               G^TGCAC
AseI          405  ATTAAT               AT^TAAT
AsnI          405  ATTAAT               AT^TAAT
AspEI         582  GACTTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII        2117  GGACC                G^G[AT]CC
AvrII        1393  CCTAGG               C^CTAGG
BalI          377  TGGCCA               TGG^CCA
BclI         1947  TGATCA               T^GATCA
BfrI         1806  CTTAAG               C^TTAAG
BglI         1357  GCCAAAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1393  CCTAGG               C^CTAGG
BlpI          663  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      663  GCTTAGC              GC^T[ACGT]AGC
BsaBI        1513  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2418  GGCGTC               G[GA]^CG[CT]C
BsaWI        2548  ACCGGA               [AT]^CCGG[AT]
BsgI         2285  CTGCAC               GTGCAG(16/14)
BsmAI        1194  GAGAC                GTCTC(1/5)
BspLU11I     1975  ACATGT               A^CATGT
BstBI        1844  TTCGAA               TT^CGAA
Bsu36I        424  CCTGAGG              CC^T[ACGT]AGG
CelII         663  GCTTAGC              GC^T[ACGT]AGC
DraI            7  TTTAAA               TTT^AAA
DsaI          440  CCATGG               C^C[GA][CT]GG
EaeI          377  TGGCCA               [CT]^GGCC[GA]
Eam1105I      582  GACTTCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        424  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      2579  ATGCAT               ATGCA^T
FbaI         1947  TGATCA               T^GATCA
Hin1I        2418  GGCGTC               G[GA]^CG[CT]C
HincII       2395  GTTAAC               GT[CT]^[GA]AC
HindII       2395  GTTAAC               GT[CT]^[GA]AC
HpaI         2395  GTTAAC               GTT^AAC
MamI         1513  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         377  TGGCCA               TGG^CCA
MscI          377  TGGCCA               TGG^CCA
MspA1I       1661  CAGCTG               C[AC]G^C[GT]G
NcoI          440  CCATGG               C^CATGG
NsiI         2579  ATGCAT               ATGCA^T
NspV         1844  TTCGAA               TT^CGAA
PpuMI        2116  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         937  GACTTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         405  ATTAAT               AT^TAAT
PstI         1306  CTGCAG               CTGCA^G
PvuII        1661  CAGCTG               CAG^CTG
SapI          202  GAAGAGC              GCTCTTC(1/4)
ScaI         2553  AGTACT               AGT^ACT
SfcI         1306  CTGCAG               C^T[GA][CT]AG
SfuI         1844  TTCGAA               TT^CGAA
StuI         2312  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AclI         1019  AACGTT               AA^CGTT
             1399  AACGTT              
AcsI          339  AAATTT               [GA]^AATT[CT]
             1335  GAATTT              
ApoI          339  AAATTT               [GA]^AATT[CT]
             1335  GAATTT              
BpmI          385  CTGGAG               CTGGAG(16/14)
             2736  CTGGAG              
BseRI         182  GAGGAG               GAGGAG(10/8)
             2068  GAGGAG              
BsiYI        1238  CCCTACAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2505  CCCAGAAATGG         
BslI         1238  CCCTACAATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2505  CCCAGAAATGG         
DraII        2116  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2708  AGGGCCC             
EcoO109I     2116  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2708  AGGGCCC             
HapII        1711  CCGG                 C^CGG
             2549  CCGG                
HgaI         2470  GACGC                GACGC(5/10)
             2419  GCGTC               
HpaII        1711  CCGG                 C^CGG
             2549  CCGG                
MslI          530  CATCTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2722  CATGAACATG          
MspI         1711  CCGG                 C^CGG
             2549  CCGG                
Psp1406I     1019  AACGTT               AA^CGTT
             1399  AACGTT              
SacI          417  GAGCTC               GAGCT^C
             2431  GAGCTC              
Tsp45I        464  GTGAC                ^GT[GC]AC
             1877  GTGAC               
XbaI         1578  TCTAGA               T^CTAGA
             2004  TCTAGA              
XcmI          746  CCACGAATTATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1358  CCAAAGAGGCAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AfaI        AluI        AlwI        Asp700I     AspHI       
BanII       BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaJI       BsiHKAI     BsmFI       Bsp1286I    
BspHI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HindIII     HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MfeI        MflI        MnlI        
MseI        MunI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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