Restriction map for pF1KB6494
 (Linear) (Six-base) MAPSORT of: KIBB6494.seq  Check: 4500  from: 1  to: 810



KIBB6494.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     686     810 
   Size:        686     124
 
AflIII A'CryG_T

Cuts at:      0     707     810 
   Size:        707     103
 
AleI CACnn'nnGTG

Cuts at:      0     226     810 
   Size:        226     584
 
ApoI r'AATT_y

Cuts at:      0     639     810 
   Size:        639     171
 
AvaI C'yCGr_G

Cuts at:      0      69     122     810 
   Size:         69      53     688

  Fragments arranged by size: 

                 688      69      53
 
BanII G_rGCy'C

Cuts at:      0     123     129     810 
   Size:        123       6     681

  Fragments arranged by size: 

                 681     123       6
 
BbvCI CC'TCA_GC

Cuts at:      0     528     810 
   Size:        528     282
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     726     810 
   Size:        726      84
 
BclI T'GATC_A

Cuts at:      0     485     810 
   Size:        485     325
 
BglII A'GATC_T

Cuts at:      0     397     810 
   Size:        397     413
 
Bme1580I G_kGCm'C

Cuts at:      0     143     761     810 
   Size:        143     618      49

  Fragments arranged by size: 

                 618     143      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     466     810 
   Size:        347     119     344

  Fragments arranged by size: 

                 347     344     119
 
Bpu10I CC'TnA_GC

Cuts at:      0     528     810 
   Size:        528     282
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     673     810 
   Size:         83     590     137

  Fragments arranged by size: 

                 590     137      83
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      24     810 
   Size:         24     786
 
BseYI C'CCAG_C

Cuts at:      0     128     810 
   Size:        128     682
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     810 
   Size:        281     529
 
BsiHKAI G_wGCw'C

Cuts at:      0     543     810 
   Size:        543     267
 
Bsp1286I G_dGCh'C

Cuts at:      0     123     129     143     543     761     810 
   Size:        123       6      14     400     218      49

  Fragments arranged by size: 

                 400     218     123      49      14       6
 
BsrDI GCAATG_nn'

Cuts at:      0     756     810 
   Size:        756      54
 
BsrFI r'CCGG_y

Cuts at:      0      49     810 
   Size:         49     761
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     138     810 
   Size:        138     672
 
BstBI TT'CG_AA

Cuts at:      0      17     810 
   Size:         17     793
 
BstYI r'GATC_y

Cuts at:      0     397     684     810 
   Size:        397     287     126

  Fragments arranged by size: 

                 397     287     126
 
BtgI C'CryG_G

Cuts at:      0     302     810 
   Size:        302     508
 
BtsI GCAGTG_nn'

Cuts at:      0     348     810 
   Size:        348     462
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     159     194     810 
   Size:        159      35     616

  Fragments arranged by size: 

                 616     159      35
 
DraI TTT'AAA

Cuts at:      0     792     810 
   Size:        792      18
 
DraIII CAC_nnn'GTG

Cuts at:      0     138     810 
   Size:        138     672
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     466     686     810 
   Size:        347     119     220     124

  Fragments arranged by size: 

                 347     220     124     119
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     530     562     810 
   Size:        530      32     248

  Fragments arranged by size: 

                 530     248      32
 
HincII GTy'rAC

Cuts at:      0     324     810 
   Size:        324     486
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     810 
   Size:        283     527
 
MslI CAynn'nnrTG

Cuts at:      0     226     810 
   Size:        226     584
 
MspA1I CmG'CkG

Cuts at:      0     159     295     699     810 
   Size:        159     136     404     111

  Fragments arranged by size: 

                 404     159     136     111
 
NaeI GCC'GGC

Cuts at:      0      51     810 
   Size:         51     759
 
NcoI C'CATG_G

Cuts at:      0     302     810 
   Size:        302     508
 
NdeI CA'TA_TG

Cuts at:      0     372     810 
   Size:        372     438
 
NgoMIV G'CCGG_C

Cuts at:      0      49     810 
   Size:         49     761
 
NspI r_CATG'y

Cuts at:      0     247     711     810 
   Size:        247     464      99

  Fragments arranged by size: 

                 464     247      99
 
PciI A'CATG_T

Cuts at:      0     707     810 
   Size:        707     103
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     607     810 
   Size:        607     203
 
PfoI T'CCnGG_A

Cuts at:      0     555     810 
   Size:        555     255
 
PvuII CAG'CTG

Cuts at:      0     159     295     699     810 
   Size:        159     136     404     111

  Fragments arranged by size: 

                 404     159     136     111
 
SexAI A'CCwGG_T

Cuts at:      0     629     810 
   Size:        629     181
 
SmaI CCC'GGG

Cuts at:      0      71     810 
   Size:         71     739
 
SmlI C'TyrA_G

Cuts at:      0      98     688     810 
   Size:         98     590     122

  Fragments arranged by size: 

                 590     122      98
 
StyI C'CwwG_G

Cuts at:      0     302     810 
   Size:        302     508
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     315     810 
   Size:        315     495
 
TatI w'GTAC_w

Cuts at:      0     145     810 
   Size:        145     665
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     342     374     567     599     810 
   Size:        342      32     193      32     211

  Fragments arranged by size: 

                 342     211     193      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     474     810 
   Size:        474     336
 
XmaI C'CCGG_G

Cuts at:      0      69     810 
   Size:         69     741
 
XmnI GAAnn'nnTTC

Cuts at:      0     460     592     810 
   Size:        460     132     218

  Fragments arranged by size: 

                 460     218     132
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AvaI    BanII    BbvCI    BciVI
     BclI    BglII Bme1580I     BpmI   Bpu10I    BpuEI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrFI   BstAPI    BstBI    BstYI
     BtgI     BtsI    CspCI     DraI   DraIII  Eco57MI    Hin4I   HincII
     MmeI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NspI
     PciI    PflMI     PfoI    PvuII    SexAI     SmaI     SmlI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NarI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
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