Restriction map for pF1KE1031
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AccI        AclI        AcsI        AgeI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
AviII       BamHI       BcgI        BciVI       BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
EagI        EclXI       Eco52I      EcoRI       EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HpaI        MamI        MfeI        MluI        MunI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII        3612  GACGTC               GACGT^C
Acc65I       1454  GGTACC               G^GTACC
AflII        1302  CTTAAG               C^TTAAG
AflIII       3753  ACACGT               A^C[GA][CT]GT
Aor51HI      2898  AGCGCT               AGC^GCT
Asp700I      2775  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1454  GGTACC               G^GTACC
AspI          988  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         568  CCTAGG               C^CTAGG
BbeI         3425  GGCGCC               GGCGC^C
BbrPI        3754  CACGTG               CAC^GTG
BclI          294  TGATCA               T^GATCA
BfrI         1302  CTTAAG               C^TTAAG
BglII         787  AGATCT               A^GATCT
BlnI          568  CCTAGG               C^CTAGG
BsmBI        2641  CGTCTC               CGTCTC(1/5)
BssSI        2608  CTCGTG               CACGAG(-5/-1)
BstEII       2062  GGTTACC              G^GT[ACGT]ACC
BstPI        2062  GGTTACC              G^GT[ACGT]ACC
DrdI         2283  GACACAGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2898  AGCGCT               AGC^GCT
EcoO65I      2062  GGTTACC              G^GT[ACGT]ACC
FbaI          294  TGATCA               T^GATCA
HindIII      4060  AAGCTT               A^AGCTT
KasI         3425  GGCGCC               G^GCGCC
KpnI         1454  GGTACC               GGTAC^C
KspI         2116  CCGCGG               CCGC^GG
NarI         3425  GGCGCC               GG^CGCC
NheI         1287  GCTAGC               G^CTAGC
NspI         1233  GCATGT               [GA]CATG^[CT]
PflFI         988  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3754  CACGTG               CAC^GTG
PmlI         3754  CACGTG               CAC^GTG
SacII        2116  CCGCGG               CCGC^GG
SapI         1393  GCTCTTC              GCTCTTC(1/4)
SbfI          729  CCTGCAGG             CCTGCA^GG
SfoI         3425  GGCGCC               GGC^GCC
SmaI         2763  CCCGGG               CCC^GGG
Sse8387I      729  CCTGCAGG             CCTGCA^GG
SspI         1900  AATATT               AAT^ATT
Tth111I       988  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2763  CCCGGG               C^CCGGG
XmaI         2763  CCCGGG               C^CCGGG
XmnI         2775  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII         407  CGCG                 CG^CG
             2117  CGCG                
AccIII       1934  TCCGGA               T^CCGGA
             3863  TCCGGA              
AcyI         3425  GGCGCC               G[GA]^CG[CT]C
             3612  GACGTC              
AhdI         1595  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3079  GACCAGCTGTC         
AspEI        1595  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3079  GACCAGCTGTC         
BbsI         3832  GAAGAC               GAAGAC(2/6)
             1079  GTCTTC              
BpuAI        3832  GAAGAC               GAAGAC(2/6)
             1079  GTCTTC              
BsaAI        1648  TACGTG               [CT]AC^GT[GA]
             3754  CACGTG              
BsaHI        3425  GGCGCC               G[GA]^CG[CT]C
             3612  GACGTC              
BsaWI        1934  TCCGGA               [AT]^CCGG[AT]
             3863  TCCGGA              
BseAI        1934  TCCGGA               T^CCGGA
             3863  TCCGGA              
BsmI         1525  GAATGC               GAATGC(1/-1)
             1722  GCATTC              
BspEI        1934  TCCGGA               T^CCGGA
             3863  TCCGGA              
BsrBI         145  CCGCTC               CCGCTC(-3/-3)
             1160  GAGCGG              
BstUI         407  CGCG                 CG^CG
             2117  CGCG                
Eam1105I     1595  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3079  GACCAGCTGTC         
EcoNI         252  CCTGCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2478  CCTGTCAGAGG         
HgaI         3711  GCGTC                GACGC(5/10)
             4119  GCGTC               
Hin1I        3425  GGCGCC               G[GA]^CG[CT]C
             3612  GACGTC              
MroI         1934  TCCGGA               T^CCGGA
             3863  TCCGGA              
MseI         1303  TTAA                 T^TAA
             2702  TTAA                
MvnI          407  CGCG                 CG^CG
             2117  CGCG                
StuI         2420  AGGCCT               AGG^CCT
             3140  AGGCCT              
Tru9I        1303  TTAA                 T^TAA
             2702  TTAA                
XcmI         1739  CCACAGGGGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3365  CCACAGACACCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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