Restriction map for pF1KE1030
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AvaII       AviII       AvrII       BanII       
BbeI        BbrPI       BbvCI       BciVI       BcnI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       BstUI       Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FauI        FseI        FspI        HaeII       HapII       
HhaI        Hin1I       HinP1I      HpaII       KasI        
KspI        MamI        MfeI        MluI        MroI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        555  GGTACC               G^GTACC
Asp718I       555  GGTACC               G^GTACC
AspI         3795  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          242  CTCGAG               C^[CT]CG[GA]G
BalI         1568  TGGCCA               TGG^CCA
BamHI         785  GGATCC               G^GATCC
BanI          555  GGTACC               G^G[CT][GA]CC
BcgI          472  GCAGCTGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI         3028  GGTCTC               GGTCTC(1/5)
BsoBI         242  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     2895  TGTACA               T^GTACA
BsrGI        2895  TGTACA               T^GTACA
Bst1107I     1280  GTATAC               GTA^TAC
BstXI         789  CCAGAAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1280  GTATAC               GTA^TAC
Cfr13I       1965  GGCCC                G^G[ACGT]CC
DraII        1964  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI         1568  TGGCCA               [CT]^GGCC[GA]
EcoO109I     1964  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I      1616  CCTTGG               C^C[AT][AT]GG
EcoT22I      2205  ATGCAT               ATGCA^T
HgaI          400  GACGC                GACGC(5/10)
HincII       1071  GTTAAC               GT[CT]^[GA]AC
HindII       1071  GTTAAC               GT[CT]^[GA]AC
HpaI         1071  GTTAAC               GTT^AAC
KpnI          555  GGTACC               GGTAC^C
MaeII        1878  ACGT                 A^CGT
MluNI        1568  TGGCCA               TGG^CCA
MscI         1568  TGGCCA               TGG^CCA
NsiI         2205  ATGCAT               ATGCA^T
PaeR7I        242  CTCGAG               C^TCGAG
PflFI        3795  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         721  GACTCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1334  CTGCAG               CTGCA^G
Sau96I       1965  GGCCC                G^G[ACGT]CC
SexAI        3887  ACCAGGT              A^CC[AT]GGT
SspBI        2895  TGTACA               T^GTACA
StuI         2762  AGGCCT               AGG^CCT
StyI         1616  CCTTGG               C^C[AT][AT]GG
TspRI        3695  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I      3795  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3264  TCTAGA               T^CTAGA
XcmI         2409  CCATGAGAAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          242  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          913  GTAGAC               GT^[AC][GT]AC
             1280  GTATAC              
AflII        1909  CTTAAG               C^TTAAG
             2395  CTTAAG              
AflIII       1182  ACATGT               A^C[GA][CT]GT
             2727  ACATGT              
Asp700I      1492  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2440  GAAGAGCTTC          
AspHI        3268  GAGCAC               G[AT]GC[AT]^C
             3880  GAGCAC              
BclI          282  TGATCA               T^GATCA
             2361  TGATCA              
BfrI         1909  CTTAAG               C^TTAAG
             2395  CTTAAG              
BlpI         1435  GCTGAGC              GC^T[ACGT]AGC
             2350  GCTAAGC             
BmyI         3268  GAGCAC               G[AGT]GC[ACT]^C
             3880  GAGCAC              
Bpu1102I     1435  GCTGAGC              GC^T[ACGT]AGC
             2350  GCTAAGC             
BseRI        2287  GAGGAG               GAGGAG(10/8)
             3250  GAGGAG              
BsgI         1991  CTGCAC               GTGCAG(16/14)
             1951  GTGCAG              
BsiHKAI      3268  GAGCAC               G[AT]GC[AT]^C
             3880  GAGCAC              
BsiYI        2672  CCAATACAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3768  CCCAGAAACGG         
BslI         2672  CCAATACAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             3768  CCCAGAAACGG         
Bsp1286I     3268  GAGCAC               G[AGT]GC[ACT]^C
             3880  GAGCAC              
BspHI          83  TCATGA               T^CATGA
             2979  TCATGA              
BspLU11I     1182  ACATGT               A^CATGT
             2727  ACATGT              
BspMI         381  GCAGGT               ACCTGC(4/8)
             1707  GCAGGT              
BsrDI         578  CATTGC               GCAATG(2/0)
             1106  GCAATG              
BstYI         785  GGATCC               [GA]^GATC[CT]
             2694  GGATCT              
CelII        1435  GCTGAGC              GC^T[ACGT]AGC
             2350  GCTAAGC             
FbaI          282  TGATCA               T^GATCA
             2361  TGATCA              
MflI          785  GGATCC               [GA]^GATC[CT]
             2694  GGATCT              
NlaIV         555  GGTACC               GG[ACGT]^[ACGT]CC
              785  GGATCC              
NspI         1182  ACATGT               [GA]CATG^[CT]
             2727  ACATGT              
PvuII         473  CAGCTG               CAG^CTG
             2881  CAGCTG              
RcaI           83  TCATGA               T^CATGA
             2979  TCATGA              
Tsp45I        398  GTGAC                ^GT[GC]AC
             3693  GTCAC               
XhoII         785  GGATCC               [GA]^GATC[CT]
             2694  GGATCT              
XmnI         1492  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2440  GAAGAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        BbsI        BbvI        BfaI        
BpmI        BpuAI       BsaJI       BsmAI       BsmFI       
BsmI        BsrI        BssKI       BstF5I      BstNI       
Cac8I       DdeI        DpnII       DraI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
PleI        RsaI        SapI        Sau3AI      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TthHB8I     
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