Restriction map for pF1KE1030
 (Linear) (Six-base) MAPSORT of: KIEE1030.seq  Check: 621  from: 1  to: 3900



KIEE1030.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:      0     914    1281    3900 
   Size:        914     367    2619

  Fragments arranged by size: 

                2619     914     367
 
Acc65I G'GTAC_C

Cuts at:      0     555    3900 
   Size:        555    3345
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     832     860     911    1778    1877    1967    2578 
   Size:        488     344      28      51     867      99      90     611

Cuts at:   2578    3224    3449    3829    3900 
   Size:        646     225     380      71

  Fragments arranged by size: 

                 867     646     611     488     380     344     225      99
                  90      71      51      28
 
AflII C'TTAA_G

Cuts at:      0    1909    2395    3900 
   Size:       1909     486    1505

  Fragments arranged by size: 

                1909    1505     486
 
AflIII A'CryG_T

Cuts at:      0    1182    2727    3900 
   Size:       1182    1545    1173

  Fragments arranged by size: 

                1545    1182    1173
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2003    3900 
   Size:       2003    1897
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1593    1968    3696    3900 
   Size:       1593     375    1728     204

  Fragments arranged by size: 

                1728    1593     375     204
 
ApoI r'AATT_y

Cuts at:      0    1561    1751    1929    2075    2095    2313    3356    3900 
   Size:       1561     190     178     146      20     218    1043     544

  Fragments arranged by size: 

                1561    1043     544     218     190     178     146      20
            
 
AvaI C'yCGr_G

Cuts at:      0     242    3900 
   Size:        242    3658
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1604    1637    3900 
   Size:       1604      33    2263

  Fragments arranged by size: 

                2263    1604      33
 
BamHI G'GATC_C

Cuts at:      0     785    3900 
   Size:        785    3115
 
BanI G'GyrC_C

Cuts at:      0     555    3900 
   Size:        555    3345
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     654     820     880    3773    3900 
   Size:        654     166      60    2893     127

  Fragments arranged by size: 

                2893     654     166     127      60
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     461     495    3900 
   Size:        461      34    3405

  Fragments arranged by size: 

                3405     461      34
 
BclI T'GATC_A

Cuts at:      0     282    2361    3900 
   Size:        282    2079    1539

  Fragments arranged by size: 

                2079    1539     282
 
BfrBI ATG'CAT

Cuts at:      0    2207    3900 
   Size:       2207    1693
 
BlpI GC'TnA_GC

Cuts at:      0    1436    2351    3900 
   Size:       1436     915    1549

  Fragments arranged by size: 

                1549    1436     915
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     433    3900 
   Size:        433    3467
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     257    3900 
   Size:        257    3643
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2470    3718    3752    3900 
   Size:       2470    1248      34     148

  Fragments arranged by size: 

                2470    1248     148      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     708    2812    3900 
   Size:        708    2104    1088

  Fragments arranged by size: 

                2104    1088     708
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3034    3900 
   Size:       3034     866
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3784    3814    3900 
   Size:       3784      30      86

  Fragments arranged by size: 

                3784      86      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2302    3265    3900 
   Size:       2302     963     635

  Fragments arranged by size: 

                2302     963     635
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1972    1976    3900 
   Size:       1972       4    1924

  Fragments arranged by size: 

                1972    1924       4
 
BsiHKAI G_wGCw'C

Cuts at:      0    3272    3884    3900 
   Size:       3272     612      16

  Fragments arranged by size: 

                3272     612      16
 
BsmI GAATG_Cn'

Cuts at:      0    1437    2207    2301    3900 
   Size:       1437     770      94    1599

  Fragments arranged by size: 

                1599    1437     770      94
 
Bsp1286I G_dGCh'C

Cuts at:      0    3272    3884    3900 
   Size:       3272     612      16

  Fragments arranged by size: 

                3272     612      16
 
BspHI T'CATG_A

Cuts at:      0      83    2979    3900 
   Size:         83    2896     921

  Fragments arranged by size: 

                2896     921      83
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     372    1698    3900 
   Size:        372    1326    2202

  Fragments arranged by size: 

                2202    1326     372
 
BsrDI GCAATG_nn'

Cuts at:      0     577    1113    3900 
   Size:        577     536    2787

  Fragments arranged by size: 

                2787     577     536
 
BsrGI T'GTAC_A

Cuts at:      0    2895    3900 
   Size:       2895    1005
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     796    3900 
   Size:        796    3104
 
BstYI r'GATC_y

Cuts at:      0     785    2694    3900 
   Size:        785    1909    1206

  Fragments arranged by size: 

                1909    1206     785
 
BstZ17I GTA'TAC

Cuts at:      0    1282    3900 
   Size:       1282    2618
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     536    3900 
   Size:        536    3364
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     713     748    3900 
   Size:        713      35    3152

  Fragments arranged by size: 

                3152     713      35
 
DraI TTT'AAA

Cuts at:      0     252     852     956    2720    3900 
   Size:        252     600     104    1764    1180

  Fragments arranged by size: 

                1764    1180     600     252     104
 
EaeI y'GGCC_r

Cuts at:      0    1568    3900 
   Size:       1568    2332
 
EarI CTCTTCn'nnn_

Cuts at:      0      73     371    1510    1685    1895    2051    2279    2435 
   Size:         73     298    1139     175     210     156     228     156

Cuts at:   2435    2645    2772    2821    3516    3900 
   Size:        210     127      49     695     384

  Fragments arranged by size: 

                1139     695     384     298     228     210     210     175
                 156     156     127      73      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     832     860     911    1778    1877    1967    2470 
   Size:        488     344      28      51     867      99      90     503

Cuts at:   2470    2578    3224    3449    3718    3752    3829    3900 
   Size:        108     646     225     269      34      77      71

  Fragments arranged by size: 

                 867     646     503     488     344     269     225     108
                  99      90      77      71      51      34      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1965    3900 
   Size:       1965    1935
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     877    1924    2266    3900 
   Size:        877    1047     342    1634

  Fragments arranged by size: 

                1634    1047     877     342
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1116    1148    1280    1312    3784    3816    3900 
   Size:       1116      32     132      32    2472      32      84

  Fragments arranged by size: 

                2472    1116     132      84      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1073    3900 
   Size:       1073    2827
 
HindIII A'AGCT_T

Cuts at:      0     136     629    1603    3900 
   Size:        136     493     974    2297

  Fragments arranged by size: 

                2297     974     493     136
 
HpaI GTT'AAC

Cuts at:      0    1073    3900 
   Size:       1073    2827
 
KpnI G_GTAC'C

Cuts at:      0     559    3900 
   Size:        559    3341
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     248    1616    3101    3900 
   Size:        248    1368    1485     799

  Fragments arranged by size: 

                1485    1368     799     248
 
MscI TGG'CCA

Cuts at:      0    1570    3900 
   Size:       1570    2330
 
MspA1I CmG'CkG

Cuts at:      0     475    1525    2883    3900 
   Size:        475    1050    1358    1017

  Fragments arranged by size: 

                1358    1050    1017     475
 
NsiI A_TGCA'T

Cuts at:      0    2209    3900 
   Size:       2209    1691
 
NspI r_CATG'y

Cuts at:      0    1186    2731    3900 
   Size:       1186    1545    1169

  Fragments arranged by size: 

                1545    1186    1169
 
PciI A'CATG_T

Cuts at:      0    1182    2727    3900 
   Size:       1182    1545    1173

  Fragments arranged by size: 

                1545    1182    1173
 
PshAI GACnn'nnGTC

Cuts at:      0     725    3900 
   Size:        725    3175
 
PsiI TTA'TAA

Cuts at:      0    1117    3900 
   Size:       1117    2783
 
PstI C_TGCA'G

Cuts at:      0    1338    3900 
   Size:       1338    2562
 
PvuII CAG'CTG

Cuts at:      0     475    2883    3900 
   Size:        475    2408    1017

  Fragments arranged by size: 

                2408    1017     475
 
SapI GCTCTTCn'nnn_

Cuts at:      0     371    2051    2435    3900 
   Size:        371    1680     384    1465

  Fragments arranged by size: 

                1680    1465     384     371
 
SexAI A'CCwGG_T

Cuts at:      0    3887    3900 
   Size:       3887      13
 
SfcI C'TryA_G

Cuts at:      0    1334    1405    2149    2435    3370    3900 
   Size:       1334      71     744     286     935     530

  Fragments arranged by size: 

                1334     935     744     530     286      71
 
SmlI C'TyrA_G

Cuts at:      0     242     723    1909    2395    2791    3900 
   Size:        242     481    1186     486     396    1109

  Fragments arranged by size: 

                1186    1109     486     481     396     242
 
StuI AGG'CCT

Cuts at:      0    2764    3900 
   Size:       2764    1136
 
StyI C'CwwG_G

Cuts at:      0    1616    3900 
   Size:       1616    2284
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     832    3900 
   Size:        832    3068
 
TatI w'GTAC_w

Cuts at:      0    1584    2895    3900 
   Size:       1584    1311    1005

  Fragments arranged by size: 

                1584    1311    1005
 
Tth111I GACn'n_nGTC

Cuts at:      0    3798    3900 
   Size:       3798     102
 
XbaI T'CTAG_A

Cuts at:      0    3264    3900 
   Size:       3264     636
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2416    3900 
   Size:       2416    1484
 
XhoI C'TCGA_G

Cuts at:      0     242    3900 
   Size:        242    3658
 
XmnI GAAnn'nnTTC

Cuts at:      0    1496    2444    3900 
   Size:       1496     948    1456

  Fragments arranged by size: 

                1496    1456     948
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII     AlfI    AlwNI     ApoI
     AvaI     BaeI    BamHI     BanI     BbsI     BcgI     BclI    BfrBI
     BlpI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
    BstXI    BstYI  BstZ17I    BtgZI    CspCI     DraI     EaeI     EarI
  Eco57MI EcoO109I     FalI    Hin4I   HincII  HindIII     HpaI     KpnI
     MmeI     MscI   MspA1I     NsiI     NspI     PciI    PshAI     PsiI
     PstI    PvuII     SapI    SexAI     SfcI     SmlI     StuI     StyI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII    BanII     BbeI
    BbvCI    BciVI     BglI    BglII Bme1580I    BmgBI     BmtI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
   Bsu36I     BtgI     BtsI     ClaI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
     KasI     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI     XmaI     ZraI
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