Restriction map for
pF1KE1027
(Circular) (Six-base) MAPSORT of: pF1KE1027.seq Check: 2572 from: 1 to: 6218
pF1KE1027 6218 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 938 1150 1397 938
Size: 212 247 5759
Fragments arranged by size:
5759 247 212
AccI GT'mk_AC
Cuts at: 3256 3256
Size: 6218
Acc65I G'GTAC_C
Cuts at: 3234 3234
Size: 6218
AclI AA'CG_TT
Cuts at: 6058 6058
Size: 6218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 971 1780 2344 2698 2794 4043 4475 4828 5490
Size: 809 564 354 96 1249 432 353 662
Cuts at: 5490 971
Size: 1699
Fragments arranged by size:
1699 1249 809 662 564 432 354 353
96
AflIII A'CryG_T
Cuts at: 3570 5370 5651 3570
Size: 1800 281 4137
Fragments arranged by size:
4137 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2123 2123
Size: 6218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2317 2317
Size: 6218
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 283 315 688 720 1435 1467 283
Size: 32 373 32 715 32 5034
Fragments arranged by size:
5034 715 373 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 336 1092 1479 1668 1776 1803 1992 2412 2835
Size: 756 387 189 108 27 189 420 423
Cuts at: 2835 3523 4961 336
Size: 688 1438 1593
Fragments arranged by size:
1593 1438 756 688 423 420 387 189
189 108 27
ApaI G_GGCC'C
Cuts at: 153 764 1063 1237 1718 2499 153
Size: 611 299 174 481 781 3872
Fragments arranged by size:
3872 781 611 481 299 174
ApaLI G'TGCA_C
Cuts at: 5056 5056
Size: 6218
ApoI r'AATT_y
Cuts at: 859 2158 2965 3222 5571 5827 859
Size: 1299 807 257 2349 256 1250
Fragments arranged by size:
2349 1299 1250 807 257 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6218
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6218
AvaI C'yCGr_G
Cuts at: 452 554 1327 2480 2492 3100 3238 452
Size: 102 773 1153 12 608 138 3432
Fragments arranged by size:
3432 1153 773 608 138 102 12
BamHI G'GATC_C
Cuts at: 1313 3243 1313
Size: 1930 4288
BanI G'GyrC_C
Cuts at: 445 938 989 1018 2022 3234 3648 3896 3931
Size: 493 51 29 1004 1212 414 248 35
Cuts at: 3931 445
Size: 2732
Fragments arranged by size:
2732 1212 1004 493 414 248 51 35
29
BanII G_rGCy'C
Cuts at: 153 764 980 1063 1073 1237 1286 1339 1718
Size: 611 216 83 10 164 49 53 379
Cuts at: 1718 2186 2499 2627 3232 4262 153
Size: 468 313 128 605 1030 2109
Fragments arranged by size:
2109 1030 611 605 468 379 313 216
164 128 83 53 49 10
BbeI G_GCGC'C
Cuts at: 3652 3900 3652
Size: 248 5970
BbsI GAAGACnn'nnnn_
Cuts at: 2428 2428
Size: 6218
BbvCI CC'TCA_GC
Cuts at: 406 2058 406
Size: 1652 4566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3075 3109 3075
Size: 34 6184
BciVI GTATCCnnnnn_n'
Cuts at: 4116 5172 6218 4116
Size: 1056 1046 4116
Fragments arranged by size:
4116 1056 1046
BclI T'GATC_A
Cuts at: 1679 3738 1679
Size: 2059 4159
BglI GCCn_nnn'nGGC
Cuts at: 141 1036 1786 3073 141
Size: 895 750 1287 3286
Fragments arranged by size:
3286 1287 895 750
BglII A'GATC_T
Cuts at: 1359 3733 5770 1359
Size: 2374 2037 1807
Fragments arranged by size:
2374 2037 1807
BlpI GC'TnA_GC
Cuts at: 1513 2845 3043 3330 1513
Size: 1332 198 287 4401
Fragments arranged by size:
4401 1332 287 198
Bme1580I G_kGCm'C
Cuts at: 153 764 1021 1063 1237 1703 1718 2360 2499
Size: 611 257 42 174 466 15 642 139
Cuts at: 2499 2539 2873 3132 3843 3936 5060 153
Size: 40 334 259 711 93 1124 1311
Fragments arranged by size:
1311 1124 711 642 611 466 334 259
257 174 139 93 42 40 15
BmgBI CAC'GTC
Cuts at: 2868 2868
Size: 6218
BmrI ACTGGGnnnn_n'
Cuts at: 2811 3430 3511 3846 5990 2811
Size: 619 81 335 2144 3039
Fragments arranged by size:
3039 2144 619 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4585 4585
Size: 6218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 765 1384 1807 1939 2128 2452 2464 3004
Size: 401 619 423 132 189 324 12 540
Cuts at: 3004 364
Size: 3578
Fragments arranged by size:
3578 619 540 423 401 324 189 132
12
Bpu10I CC'TnA_GC
Cuts at: 145 406 2058 2185 145
Size: 261 1652 127 4178
Fragments arranged by size:
4178 1652 261 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 2875 3394 4273 4987 5285 1036
Size: 1839 519 879 714 298 1969
Fragments arranged by size:
1969 1839 879 714 519 298
BsaI GGTCTCn'nnnn_
Cuts at: 5922 5922
Size: 6218
BsaAI yAC'GTr
Cuts at: 2301 3571 2301
Size: 1270 4948
BsaBI GATnn'nnATC
Cuts at: 6 3732 5769 6
Size: 3726 2037 455
Fragments arranged by size:
3726 2037 455
BsaHI Gr'CG_yC
Cuts at: 3649 3897 4599 6095 3649
Size: 248 702 1496 3772
Fragments arranged by size:
3772 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3407 3928 4606 5017 5164 5438 3407
Size: 521 678 411 147 274 4187
Fragments arranged by size:
4187 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 688 718 1186 1216 1435 1465 688
Size: 30 468 30 219 30 5441
Fragments arranged by size:
5441 468 219 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 661 739 742 745 748 896 913 976
Size: 288 78 3 3 3 148 17 63
Cuts at: 976 1096 1126 1129 1132 1156 1178 1210 1329
Size: 120 30 3 3 24 22 32 119
Cuts at: 1329 1456 1459 2239 2266 2287 373
Size: 127 3 780 27 21 4304
Fragments arranged by size:
4304 780 288 148 127 120 119 78
63 32 30 27 24 22 21 17
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 578 1287 1668 1734 2368 2569 3151 3643 4401
Size: 709 381 66 634 201 582 492 758
Cuts at: 4401 5066 5474 578
Size: 665 408 1322
Fragments arranged by size:
1322 758 709 665 634 582 492 408
381 201 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 2539 2821 3124 3157 2446
Size: 93 282 303 33 5507
Fragments arranged by size:
5507 303 282 93 33
BsiEI CG_ry'CG
Cuts at: 104 489 780 1295 3760 3806 5036 104
Size: 385 291 515 2465 46 1230 1286
Fragments arranged by size:
2465 1286 1230 515 385 291 46
BsiHKAI G_wGCw'C
Cuts at: 633 677 2186 3232 4010 4200 5060 633
Size: 44 1509 1046 778 190 860 1791
Fragments arranged by size:
1791 1509 1046 860 778 190 44
BsmI GAATG_Cn'
Cuts at: 1939 2789 1939
Size: 850 5368
BsmBI CGTCTCn'nnnn_
Cuts at: 764 764
Size: 6218
Bsp1286I G_dGCh'C
Cuts at: 153 633 677 764 980 1021 1063 1073 1237
Size: 480 44 87 216 41 42 10 164
Cuts at: 1237 1286 1339 1703 1718 2186 2360 2499 2539
Size: 49 53 364 15 468 174 139 40
Cuts at: 2539 2627 2873 3132 3232 3843 3936 4010 4200
Size: 88 246 259 100 611 93 74 190
Cuts at: 4200 4262 5060 153
Size: 62 798 1311
Fragments arranged by size:
1311 798 611 480 468 364 259 246
216 190 174 164 139 100 93 88
87 74 62 53 49 44 42 41
40 15 10
BspHI T'CATG_A
Cuts at: 4650 4650
Size: 6218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 938 1150 1397 2000 3268 3482 3784 4165 938
Size: 212 247 603 1268 214 302 381 2991
Fragments arranged by size:
2991 1268 603 381 302 247 214 212
BsrBI CCG'CTC
Cuts at: 50 683 815 1512 1755 2454 2648 2988 4510
Size: 633 132 697 243 699 194 340 1522
Cuts at: 4510 4564 6048 6213 50
Size: 54 1484 165 55
Fragments arranged by size:
1522 1484 699 697 633 340 243 194
165 132 55 54
BsrDI GCAATG_nn'
Cuts at: 4130 4130
Size: 6218
BsrFI r'CCGG_y
Cuts at: 1609 2009 4216 4397 1609
Size: 400 2207 181 3430
Fragments arranged by size:
3430 2207 400 181
BssHII G'CGCG_C
Cuts at: 1856 1856
Size: 6218
BssSI C'ACGA_G
Cuts at: 4489 5197 4489
Size: 708 5510
BstAPI GCAn_nnn'nTGC
Cuts at: 2835 3595 2835
Size: 760 5458
BstBI TT'CG_AA
Cuts at: 4579 4579
Size: 6218
BstXI CCAn_nnnn'nTGG
Cuts at: 3159 3159
Size: 6218
BstYI r'GATC_y
Cuts at: 1 1313 1359 1562 3178 3243 3710 3733 4068
Size: 1312 46 203 1616 65 467 23 335
Cuts at: 4068 4314 4620 4632 4718 5770 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1616 1312 1052 467 449 335 306 246
203 86 65 46 23 12
Bsu36I CC'TnA_GG
Cuts at: 1079 1154 1190 1079
Size: 75 36 6107
Fragments arranged by size:
6107 75 36
BtgI C'CryG_G
Cuts at: 107 305 483 489 1518 1781 2664 2852 2873
Size: 198 178 6 1029 263 883 188 21
Cuts at: 2873 107
Size: 3452
Fragments arranged by size:
3452 1029 883 263 198 188 178 21
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 545 1664 4168 4349 4512 545
Size: 1119 2504 181 163 2251
Fragments arranged by size:
2504 2251 1119 181 163
BtsI GCAGTG_nn'
Cuts at: 325 1180 1219 1499 1627 1729 2537 3461 325
Size: 855 39 280 128 102 808 924 3082
Fragments arranged by size:
3082 924 855 808 280 128 102 39
DraI TTT'AAA
Cuts at: 3217 3217
Size: 6218
DraIII CAC_nnn'GTG
Cuts at: 637 2517 2819 637
Size: 1880 302 4036
Fragments arranged by size:
4036 1880 302
DrdI GACnn_nn'nnGTC
Cuts at: 3924 5268 3924
Size: 1344 4874
EaeI y'GGCC_r
Cuts at: 486 1292 1778 2399 3803 3977 4368 4395 5740
Size: 806 486 621 1404 174 391 27 1345
Cuts at: 5740 486
Size: 964
Fragments arranged by size:
1404 1345 964 806 621 486 391 174
27
EagI C'GGCC_G
Cuts at: 486 1292 3803 486
Size: 806 2511 2901
Fragments arranged by size:
2901 2511 806
EarI CTCTTCn'nnn_
Cuts at: 275 890 2118 2198 2273 2756 4241 4451 275
Size: 615 1228 80 75 483 1485 210 2042
Fragments arranged by size:
2042 1485 1228 615 483 210 80 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 780 1417 4426 5168 5314 6028 780
Size: 637 3009 742 146 714 970
Fragments arranged by size:
3009 970 742 714 637 146
EcoICRI GAG'CTC
Cuts at: 2184 3230 2184
Size: 1046 5172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 765 971 1384 1780 1807 1939 2128 2344
Size: 401 206 413 396 27 132 189 216
Cuts at: 2344 2452 2464 2698 2794 3004 4043 4475 4828
Size: 108 12 234 96 210 1039 432 353
Cuts at: 4828 5490 364
Size: 662 1092
Fragments arranged by size:
1092 1039 662 432 413 401 396 353
234 216 210 206 189 132 108 96
27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 712 1130 1157 1393 2893 712
Size: 418 27 236 1500 4037
Fragments arranged by size:
4037 1500 418 236 27
EcoO109I rG'GnC_Cy
Cuts at: 149 150 273 536 760 761 1037 1059 1060
Size: 1 123 263 224 1 276 22 1
Cuts at: 1060 1150 1476 1548 1570 1714 1715 1884 2502
Size: 90 326 72 22 144 1 169 618
Cuts at: 2502 2529 2798 3357 149
Size: 27 269 559 3010
Fragments arranged by size:
3010 618 559 326 276 269 263 224
169 144 123 90 72 27 22 22
1 1 1 1
EcoRI G'AATT_C
Cuts at: 3222 3222
Size: 6218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4237 4237
Size: 6218
FspI TGC'GCA
Cuts at: 3999 3999
Size: 6218
HaeII r_GCGC'y
Cuts at: 705 789 949 1963 2235 2852 3053 3652 3900
Size: 84 160 1014 272 617 201 599 248
Cuts at: 3900 5130 5901 705
Size: 1230 771 1022
Fragments arranged by size:
1230 1022 1014 771 617 599 272 248
201 160 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1186 1218 2109 2141 2887 2919 4060 4090 4092
Size: 32 891 32 746 32 1141 30 2
Cuts at: 4092 4122 1186
Size: 30 3282
Fragments arranged by size:
3282 1141 891 746 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3257 3257
Size: 6218
KasI G'GCGC_C
Cuts at: 3648 3896 3648
Size: 248 5970
KpnI G_GTAC'C
Cuts at: 3238 3238
Size: 6218
MluI A'CGCG_T
Cuts at: 5651 5651
Size: 6218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1088 4977 5161 1088
Size: 3889 184 2145
Fragments arranged by size:
3889 2145 184
MscI TGG'CCA
Cuts at: 2401 3979 2401
Size: 1578 4640
MslI CAynn'nnrTG
Cuts at: 4207 4334 4207
Size: 127 6091
MspA1I CmG'CkG
Cuts at: 307 351 485 500 743 782 1291 1320 1668
Size: 44 134 15 243 39 509 29 348
Cuts at: 1668 1783 1803 1827 2148 2442 2460 2615 3018
Size: 115 20 24 321 294 18 155 403
Cuts at: 3018 3329 3643 4003 4787 5032 307
Size: 311 314 360 784 245 1493
Fragments arranged by size:
1493 784 509 403 360 348 321 314
311 294 245 243 155 134 115 44
39 29 24 20 18 15
NaeI GCC'GGC
Cuts at: 1611 4399 1611
Size: 2788 3430
NarI GG'CG_CC
Cuts at: 3649 3897 3649
Size: 248 5970
NcoI C'CATG_G
Cuts at: 107 1518 2873 107
Size: 1411 1355 3452
Fragments arranged by size:
3452 1411 1355
NgoMIV G'CCGG_C
Cuts at: 1609 4397 1609
Size: 2788 3430
NspI r_CATG'y
Cuts at: 3271 3771 5374 3271
Size: 500 1603 4115
Fragments arranged by size:
4115 1603 500
PasI CC'CwG_GG
Cuts at: 1230 1473 1711 1230
Size: 243 238 5737
Fragments arranged by size:
5737 243 238
PciI A'CATG_T
Cuts at: 5370 5370
Size: 6218
PflMI CCAn_nnn'nTGG
Cuts at: 1107 1668 2093 1107
Size: 561 425 5232
Fragments arranged by size:
5232 561 425
PfoI T'CCnGG_A
Cuts at: 275 2162 2429 2981 275
Size: 1887 267 552 3512
Fragments arranged by size:
3512 1887 552 267
PmeI GTTT'AAAC
Cuts at: 3217 3217
Size: 6218
PmlI CAC'GTG
Cuts at: 2301 2301
Size: 6218
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 688 720 1435 1467 4664 4696 688
Size: 32 715 32 3197 32 2210
Fragments arranged by size:
3197 2210 715 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 273 1548 1884 273
Size: 1275 336 4607
Fragments arranged by size:
4607 1275 336
PspOMI G'GGCC_C
Cuts at: 149 760 1059 1233 1714 2495 149
Size: 611 299 174 481 781 3872
Fragments arranged by size:
3872 781 611 481 299 174
PstI C_TGCA'G
Cuts at: 290 317 332 1275 1394 1427 1494 1808 1949
Size: 27 15 943 119 33 67 314 141
Cuts at: 1949 2786 3062 3265 3950 290
Size: 837 276 203 685 2558
Fragments arranged by size:
2558 943 837 685 314 276 203 141
119 67 33 27 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6218
PvuII CAG'CTG
Cuts at: 500 743 1320 1803 1827 2148 2442 2460 3643
Size: 243 577 483 24 321 294 18 1183
Cuts at: 3643 4003 500
Size: 360 2715
Fragments arranged by size:
2715 1183 577 483 360 321 294 243
24 18
RsrII CG'GwC_CG
Cuts at: 4413 4413
Size: 6218
SacI G_AGCT'C
Cuts at: 2186 3232 2186
Size: 1046 5172
SacII CC_GC'GG
Cuts at: 308 486 1784 308
Size: 178 1298 4742
Fragments arranged by size:
4742 1298 178
SalI G'TCGA_C
Cuts at: 3255 3255
Size: 6218
SapI GCTCTTCn'nnn_
Cuts at: 275 2273 4241 4451 275
Size: 1998 1968 210 2042
Fragments arranged by size:
2042 1998 1968 210
SbfI CC_TGCA'GG
Cuts at: 1427 3265 1427
Size: 1838 4380
ScaI AGT'ACT
Cuts at: 5735 5735
Size: 6218
SfcI C'TryA_G
Cuts at: 33 286 313 328 1271 1390 1423 1490 1804
Size: 253 27 15 943 119 33 67 314
Cuts at: 1804 1945 2332 2782 2879 3058 3261 3946 4914
Size: 141 387 450 97 179 203 685 968
Cuts at: 4914 5105 33
Size: 191 1146
Fragments arranged by size:
1146 968 943 685 450 387 314 253
203 191 179 141 119 97 67 33
27 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 1786 3073 1786
Size: 1287 4931
SfoI GGC'GCC
Cuts at: 3650 3898 3650
Size: 248 5970
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6218
SmaI CCC'GGG
Cuts at: 556 1329 3102 3240 556
Size: 773 1773 138 3534
Fragments arranged by size:
3534 1773 773 138
SmlI C'TyrA_G
Cuts at: 1051 2890 3373 4288 5002 5264 1051
Size: 1839 483 915 714 262 2005
Fragments arranged by size:
2005 1839 915 714 483 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6218
SphI G_CATG'C
Cuts at: 3271 3771 3271
Size: 500 5718
StyI C'CwwG_G
Cuts at: 107 664 1064 1101 1436 1518 1566 2873 3352
Size: 557 400 37 335 82 48 1307 479
Cuts at: 3352 3706 107
Size: 354 2619
Fragments arranged by size:
2619 1307 557 479 400 354 335 82
48 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2382 2547 2830 3801 3934 4393 4602 2382
Size: 165 283 971 133 459 209 3998
Fragments arranged by size:
3998 971 459 283 209 165 133
TatI w'GTAC_w
Cuts at: 5733 5733
Size: 6218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5735 5767 5735
Size: 32 6186
Tth111I GACn'n_nGTC
Cuts at: 4015 4015
Size: 6218
XbaI T'CTAG_A
Cuts at: 2133 3249 2133
Size: 1116 5102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 210 1629 2859 2979 3713 210
Size: 1419 1230 120 734 2715
Fragments arranged by size:
2715 1419 1230 734 120
XmaI C'CCGG_G
Cuts at: 554 1327 3100 3238 554
Size: 773 1773 138 3534
Fragments arranged by size:
3534 1773 773 138
XmnI GAAnn'nnTTC
Cuts at: 1537 1537
Size: 6218
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI AvrII BaeI
BfrBI BmtI BsiWI BspEI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI