Restriction map for pF1KE1023
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BciVI       BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII        1173  GACGTC               GACGT^C
AcsI          554  AAATTC               [GA]^AATT[CT]
AflIII       2021  ACGTGT               A^C[GA][CT]GT
ApoI          554  AAATTC               [GA]^AATT[CT]
BalI          375  TGGCCA               TGG^CCA
BamHI        2226  GGATCC               G^GATCC
BbsI          196  GAAGAC               GAAGAC(2/6)
BcgI         1948  GCAGCTAGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1559  TGATCA               T^GATCA
BglI          894  GCCTCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         582  AGATCT               A^GATCT
BpuAI         196  GAAGAC               GAAGAC(2/6)
BsgI          314  GTGCAG               GTGCAG(16/14)
BsmBI        2018  GAGACG               CGTCTC(1/5)
BspHI        2163  TCATGA               T^CATGA
BsrBI        2252  CCGCTC               CCGCTC(-3/-3)
BsrFI         507  GCCGGC               [GA]^CCGG[CT]
BssSI         108  CTCGTG               CACGAG(-5/-1)
BstAPI       1845  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         732  CCAAGTTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         2209  GCGC                 GCG^C
Cfr10I        507  GCCGGC               [GA]^CCGG[CT]
EarI          613  GAAGAG               CTCTTC(1/4)
EcoNI         994  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I        53  CCATGG               C^C[AT][AT]GG
FbaI         1559  TGATCA               T^GATCA
HaeII        2208  AGCGCC               [GA]GCGC^[CT]
HgaI         2275  GACGC                GACGC(5/10)
HhaI         2209  GCGC                 GCG^C
HinP1I       2209  GCGC                 G^CGC
HindIII      2062  AAGCTT               A^AGCTT
Ksp632I       613  GAAGAG               CTCTTC(1/4)
MluNI         375  TGGCCA               TGG^CCA
MscI          375  TGGCCA               TGG^CCA
MslI         1557  CATGATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          507  GCCGGC               GCC^GGC
NcoI           53  CCATGG               C^CATGG
NgoMIV        507  GCCGGC               G^CCGGC
NheI         1951  GCTAGC               G^CTAGC
PflMI        2118  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2163  TCATGA               T^CATGA
SapI          613  GAAGAGC              GCTCTTC(1/4)
ScaI          304  AGTACT               AGT^ACT
SphI           35  GCATGC               GCATG^C
StyI           53  CCATGG               C^C[AT][AT]GG
TaqI          787  TCGA                 T^CGA
TthHB8I       787  TCGA                 T^CGA
Van91I       2118  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          889  CCAGTGCCTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI          514  GTAGAC               GT^[AC][GT]AC
             2047  GTAGAC              
AcyI         1173  GACGTC               G[GA]^CG[CT]C
             2275  GACGCC              
AlwNI        1748  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2119  CAGGCCCTG           
AvaI         2095  CTCGGG               C^[CT]CG[GA]G
             2174  CCCGAG              
BanI         2078  GGCACC               G^G[CT][GA]CC
             2247  GGTGCC              
BbvCI        1745  CCTCAGC              CCTCAGC(-5/-2)
             2008  CCTCAGC             
BcnI         1654  CCGGG                CC^[GC]GG
             2146  CCCGG               
BsaHI        1173  GACGTC               G[GA]^CG[CT]C
             2275  GACGCC              
BsmAI         186  GAGAC                GTCTC(1/5)
             2018  GAGAC               
BsoBI        2095  CTCGGG               C^[CT]CG[GA]G
             2174  CCCGAG              
EaeI          375  TGGCCA               [CT]^GGCC[GA]
             1766  CGGCCA              
FauI         1201  CCCGC                CCCGC(4/6)
             2251  CCCGC               
Hin1I        1173  GACGTC               G[GA]^CG[CT]C
             2275  GACGCC              
MaeII        1174  ACGT                 A^CGT
             2021  ACGT                
NciI         1654  CCGGG                CC^[GC]GG
             2146  CCCGG               
PpuMI         326  AGGTCCC              [GA]G^G[AT]CC[CT]
             1656  GGGTCCC             
SmlI          586  CTTGAG               C^T[CT][GA]AG
             1316  CTCAAG              
Tsp509I       246  AATT                 ^AATT
              555  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        ApaI        
AspHI       AvaII       BanII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       XhoII       
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