Restriction map for pF1KB5628
##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AccI        AclI        AflII       AocI        AscI        
AviII       BbvCI       BcgI        BciVI       BclI        
BfrI        BsaBI       BsiWI       Bsp1407I    BspDI       
BsrGI       BssHII      Bst1107I    BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        Eco81I      EcoRV       
FbaI        FseI        FspI        HindIII     HpaI        
MamI        MfeI        MluI        MunI        NcoI        
NdeI        NruI        PacI        PmeI        PshAI       
Psp1406I    PvuI        RsrII       SalI        ScaI        
SfiI        SgrAI       SnaBI       SpeI        SspBI       
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AatII        2377  GACGTC               GACGT^C
Acc65I       4114  GGTACC               G^GTACC
AgeI         3128  ACCGGT               A^CCGGT
Aor51HI      1604  AGCGCT               AGC^GCT
Asp700I      2202  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      4114  GGTACC               G^GTACC
AvrII        1503  CCTAGG               C^CTAGG
BbsI         3634  GAAGAC               GAAGAC(2/6)
BlnI         1503  CCTAGG               C^CTAGG
BpuAI        3634  GAAGAC               GAAGAC(2/6)
BsaI          182  GAGACC               GGTCTC(1/5)
BsmBI        3915  CGTCTC               CGTCTC(1/5)
BspLU11I     2402  ACATGT               A^CATGT
DraI          675  TTTAAA               TTT^AAA
DrdI          635  GACTAGAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1604  AGCGCT               AGC^GCT
EcoT22I      2490  ATGCAT               ATGCA^T
KpnI         4114  GGTACC               GGTAC^C
NheI         2367  GCTAGC               G^CTAGC
NotI         2998  GCGGCCGC             GC^GGCCGC
NsiI         2490  ATGCAT               ATGCA^T
PaeR7I       1338  CTCGAG               C^TCGAG
PinAI        3128  ACCGGT               A^CCGGT
SbfI         2846  CCTGCAGG             CCTGCA^GG
Sse8387I     2846  CCTGCAGG             CCTGCA^GG
SspI         2246  AATATT               AAT^ATT
XhoI         1338  CTCGAG               C^TCGAG
XmnI         2202  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccIII       1431  TCCGGA               T^CCGGA
             2965  TCCGGA              
AflIII       1013  ACGTGT               A^C[GA][CT]GT
             2402  ACATGT              
AhdI         2956  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3805  GACAGCCCGTC         
Alw44I       1086  GTGCAC               G^TGCAC
             2516  GTGCAC              
ApaLI        1086  GTGCAC               G^TGCAC
             2516  GTGCAC              
AseI          246  ATTAAT               AT^TAAT
             3544  ATTAAT              
AsnI          246  ATTAAT               AT^TAAT
             3544  ATTAAT              
AspEI        2956  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3805  GACAGCCCGTC         
BalI         3191  TGGCCA               TGG^CCA
             3710  TGGCCA              
BamHI        3312  GGATCC               G^GATCC
             3480  GGATCC              
BglI         1352  GCCCCGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2128  GCCAGCTGGGC         
BglII        2426  AGATCT               A^GATCT
             3944  AGATCT              
BseAI        1431  TCCGGA               T^CCGGA
             2965  TCCGGA              
BspEI        1431  TCCGGA               T^CCGGA
             2965  TCCGGA              
BspMI        3077  ACCTGC               ACCTGC(4/8)
             2535  GCAGGT              
BsrBI        1463  CCGCTC               CCGCTC(-3/-3)
             2977  GAGCGG              
BssSI         726  CACGAG               CACGAG(-5/-1)
             2694  CTCGTG              
BstBI         304  TTCGAA               TT^CGAA
             1856  TTCGAA              
BstEII        813  GGTGACC              G^GT[ACGT]ACC
              942  GGTTACC             
BstPI         813  GGTGACC              G^GT[ACGT]ACC
              942  GGTTACC             
DraIII        119  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              274  CACATGGTG           
Eam1105I     2956  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3805  GACAGCCCGTC         
EcoO65I       813  GGTGACC              G^GT[ACGT]ACC
              942  GGTTACC             
EcoRI        1120  GAATTC               G^AATTC
             2206  GAATTC              
HincII       2022  GTTGAC               GT[CT]^[GA]AC
             2344  GTCAAC              
HindII       2022  GTTGAC               GT[CT]^[GA]AC
             2344  GTCAAC              
KspI          765  CCGCGG               CCGC^GG
             3002  CCGCGG              
MluNI        3191  TGGCCA               TGG^CCA
             3710  TGGCCA              
MroI         1431  TCCGGA               T^CCGGA
             2965  TCCGGA              
MscI         3191  TGGCCA               TGG^CCA
             3710  TGGCCA              
NaeI           88  GCCGGC               GCC^GGC
             2102  GCCGGC              
NgoMIV         88  GCCGGC               G^CCGGC
             2102  GCCGGC              
NspV          304  TTCGAA               TT^CGAA
             1856  TTCGAA              
PshBI         246  ATTAAT               AT^TAAT
             3544  ATTAAT              
SacII         765  CCGCGG               CCGC^GG
             3002  CCGCGG              
SapI         1299  GAAGAGC              GCTCTTC(1/4)
             2644  GAAGAGC             
SexAI        3638  ACCTGGT              A^CC[AT]GGT
             4110  ACCAGGT             
SfuI          304  TTCGAA               TT^CGAA
             1856  TTCGAA              
XcmI         1106  CCAATGGCTGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3194  CCAGATACGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AspI        AvaI        AvaII       BanI        
BanII       BbeI        BbrPI       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaHI       BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspHI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        SphI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XhoII       XmaCI       XmaI        
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