Restriction map for pF1KB5628
 (Linear) (Six-base) MAPSORT of: KIBB5628.seq  Check: 2106  from: 1  to: 4128



KIBB5628.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AatII G_ACGT'C

Cuts at:      0    2381    4128 
   Size:       2381    1747
 
Acc65I G'GTAC_C

Cuts at:      0    4114    4128 
   Size:       4114      14
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     379     885    2059    2488    4128 
   Size:        308      71     506    1174     429    1640

  Fragments arranged by size: 

                1640    1174     506     429     308      71
 
AfeI AGC'GCT

Cuts at:      0    1606    4128 
   Size:       1606    2522
 
AflIII A'CryG_T

Cuts at:      0    1013    2402    4128 
   Size:       1013    1389    1726

  Fragments arranged by size: 

                1726    1389    1013
 
AgeI A'CCGG_T

Cuts at:      0    3128    4128 
   Size:       3128    1000
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2961    3810    4128 
   Size:       2961     849     318

  Fragments arranged by size: 

                2961     849     318
 
AleI CACnn'nnGTG

Cuts at:      0     836    2585    3172    4128 
   Size:        836    1749     587     956

  Fragments arranged by size: 

                1749     956     836     587
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4081    4128 
   Size:       4081      47
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3674    3706    4128 
   Size:       3674      32     422

  Fragments arranged by size: 

                3674     422      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1484    2421    3706    4128 
   Size:       1484     937    1285     422

  Fragments arranged by size: 

                1484    1285     937     422
 
ApaI G_GGCC'C

Cuts at:      0    1206    1424    2298    3893    4128 
   Size:       1206     218     874    1595     235

  Fragments arranged by size: 

                1595    1206     874     235     218
 
ApaLI G'TGCA_C

Cuts at:      0    1086    2516    4128 
   Size:       1086    1430    1612

  Fragments arranged by size: 

                1612    1430    1086
 
ApoI r'AATT_y

Cuts at:      0     434    1120    1888    2206    3381    4128 
   Size:        434     686     768     318    1175     747

  Fragments arranged by size: 

                1175     768     747     686     434     318
 
AseI AT'TA_AT

Cuts at:      0     247    3545    4128 
   Size:        247    3298     583

  Fragments arranged by size: 

                3298     583     247
 
AvaI C'yCGr_G

Cuts at:      0      77     459    1338    2297    2863    3822    3868    4128 
   Size:         77     382     879     959     566     959      46     260

  Fragments arranged by size: 

                 959     959     879     566     382     260      77      46
            
 
AvrII C'CTAG_G

Cuts at:      0    1503    4128 
   Size:       1503    2625
 
BamHI G'GATC_C

Cuts at:      0    3312    3480    4128 
   Size:       3312     168     648

  Fragments arranged by size: 

                3312     648     168
 
BanI G'GyrC_C

Cuts at:      0     117     779     991    1390    1527    1573    2029    2105 
   Size:        117     662     212     399     137      46     456      76

Cuts at:   2105    2859    3262    3934    4114    4128 
   Size:        754     403     672     180      14

  Fragments arranged by size: 

                 754     672     662     456     403     399     212     180
                 137     117      76      46      14
 
BanII G_rGCy'C

Cuts at:      0     154     488     733    1206    1339    1424    1450    1552 
   Size:        154     334     245     473     133      85      26     102

Cuts at:   1552    1885    2298    2809    3251    3829    3893    4128 
   Size:        333     413     511     442     578      64     235

  Fragments arranged by size: 

                 578     511     473     442     413     334     333     245
                 235     154     133     102      85      64      26
 
BbeI G_GCGC'C

Cuts at:      0     783     995    2033    2109    3266    4128 
   Size:        783     212    1038      76    1157     862

  Fragments arranged by size: 

                1157    1038     862     783     212      76
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3641    4128 
   Size:       3641     487
 
BfrBI ATG'CAT

Cuts at:      0    2492    4128 
   Size:       2492    1636
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1358    2134    4128 
   Size:       1358     776    1994

  Fragments arranged by size: 

                1994    1358     776
 
BglII A'GATC_T

Cuts at:      0    2426    3944    4128 
   Size:       2426    1518     184

  Fragments arranged by size: 

                2426    1518     184
 
BlpI GC'TnA_GC

Cuts at:      0     130     847    3828    4128 
   Size:        130     717    2981     300

  Fragments arranged by size: 

                2981     717     300     130
 
Bme1580I G_kGCm'C

Cuts at:      0     916    1090    1206    1395    1424    1532    1576    2298 
   Size:        916     174     116     189      29     108      44     722

Cuts at:   2298    2520    3893    3937    4128 
   Size:        222    1373      44     191

  Fragments arranged by size: 

                1373     916     722     222     191     189     174     116
                 108      44      44      29
 
BmgBI CAC'GTC

Cuts at:      0    1931    4128 
   Size:       1931    2197
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1427    3900    4128 
   Size:       1427    2473     228

  Fragments arranged by size: 

                2473    1427     228
 
BmtI G_CTAG'C

Cuts at:      0    2371    4128 
   Size:       2371    1757
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3777    3977    4128 
   Size:       3777     200     151

  Fragments arranged by size: 

                3777     200     151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     760     884    2568    3375    3683    3819    4104 
   Size:        200     560     124    1684     807     308     136     285

Cuts at:   4104    4128 
   Size:         24

  Fragments arranged by size: 

                1684     807     560     308     285     200     136     124
                  24
 
Bpu10I CC'TnA_GC

Cuts at:      0    2065    3625    4128 
   Size:       2065    1560     503

  Fragments arranged by size: 

                2065    1560     503
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1563    2014    3127    4128 
   Size:       1563     451    1113    1001

  Fragments arranged by size: 

                1563    1113    1001     451
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     176    4128 
   Size:        176    3952
 
BsaAI yAC'GTr

Cuts at:      0    1014    1623    4008    4128 
   Size:       1014     609    2385     120

  Fragments arranged by size: 

                2385    1014     609     120
 
BsaHI Gr'CG_yC

Cuts at:      0     293     780     992    1129    1226    2030    2106    2378 
   Size:        293     487     212     137      97     804      76     272

Cuts at:   2378    2762    3263    4128 
   Size:        384     501     865

  Fragments arranged by size: 

                 865     804     501     487     384     293     272     212
                 137      97      76
 
BsaWI w'CCGG_w

Cuts at:      0    1431    2965    3128    4128 
   Size:       1431    1534     163    1000

  Fragments arranged by size: 

                1534    1431    1000     163
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     691     721    1624    1654    3674    3704    4128 
   Size:        691      30     903      30    2020      30     424

  Fragments arranged by size: 

                2020     903     691     424      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     997    1000    1309    1560    2133    3319    3577    4128 
   Size:        997       3     309     251     573    1186     258     551

  Fragments arranged by size: 

                1186     997     573     551     309     258     251       3
            
 
BseYI C'CCAG_C

Cuts at:      0      10    1394    1533    2086    2132    2363    2542    2649 
   Size:         10    1384     139     553      46     231     179     107

Cuts at:   2649    3764    3883    4128 
   Size:       1115     119     245

  Fragments arranged by size: 

                1384    1115     553     245     231     179     139     119
                 107      46      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862    2113    2249    3028    3882    4128 
   Size:        862    1251     136     779     854     246

  Fragments arranged by size: 

                1251     862     854     779     246     136
 
BsiEI CG_ry'CG

Cuts at:      0     300     524     887     924    1094    2631    3002    3750 
   Size:        300     224     363      37     170    1537     371     748

Cuts at:   3750    4128 
   Size:        378

  Fragments arranged by size: 

                1537     748     378     371     363     300     224     170
                  37
 
BsiHKAI G_wGCw'C

Cuts at:      0     131     262     733     853    1090    1339    1410    1552 
   Size:        131     131     471     120     237     249      71     142

Cuts at:   1552    1628    2520    2716    2744    4007    4128 
   Size:         76     892     196      28    1263     121

  Fragments arranged by size: 

                1263     892     471     249     237     196     142     131
                 131     121     120      76      71      28
 
BsmI GAATG_Cn'

Cuts at:      0    1791    1829    2494    2894    4128 
   Size:       1791      38     665     400    1234

  Fragments arranged by size: 

                1791    1234     665     400      38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3921    4128 
   Size:       3921     207
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     154     262     488     733     853     916    1090 
   Size:        131      23     108     226     245     120      63     174

Cuts at:   1090    1206    1339    1395    1410    1424    1450    1532    1552 
   Size:        116     133      56      15      14      26      82      20

Cuts at:   1552    1576    1628    1885    2298    2520    2716    2744    2809 
   Size:         24      52     257     413     222     196      28      65

Cuts at:   2809    3251    3829    3893    3937    4007    4128 
   Size:        442     578      64      44      70     121

  Fragments arranged by size: 

                 578     442     413     257     245     226     222     196
                 174     133     131     121     120     116     108      82
                  70      65      64      63      56      52      44      28
                  26      24      23      20      15      14
 
BspEI T'CCGG_A

Cuts at:      0    1431    2965    4128 
   Size:       1431    1534    1163

  Fragments arranged by size: 

                1534    1431    1163
 
BspHI T'CATG_A

Cuts at:      0     140     228     609     645    2438    4128 
   Size:        140      88     381      36    1793    1690

  Fragments arranged by size: 

                1793    1690     381     140      88      36
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2526    3086    4128 
   Size:       2526     560    1042

  Fragments arranged by size: 

                2526    1042     560
 
BsrBI CCG'CTC

Cuts at:      0    1465    2979    4128 
   Size:       1465    1514    1149

  Fragments arranged by size: 

                1514    1465    1149
 
BsrDI GCAATG_nn'

Cuts at:      0      31    1061    2079    4128 
   Size:         31    1030    1018    2049

  Fragments arranged by size: 

                2049    1030    1018      31
 
BsrFI r'CCGG_y

Cuts at:      0      88     923    2102    3128    4128 
   Size:         88     835    1179    1026    1000

  Fragments arranged by size: 

                1179    1026    1000     835      88
 
BssSI C'ACGA_G

Cuts at:      0     726    2694    4128 
   Size:        726    1968    1434

  Fragments arranged by size: 

                1968    1434     726
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     664    1703    2421    4128 
   Size:        664    1039     718    1707

  Fragments arranged by size: 

                1707    1039     718     664
 
BstBI TT'CG_AA

Cuts at:      0     305    1857    4128 
   Size:        305    1552    2271

  Fragments arranged by size: 

                2271    1552     305
 
BstEII G'GTnAC_C

Cuts at:      0     813     942    4128 
   Size:        813     129    3186

  Fragments arranged by size: 

                3186     813     129
 
BstYI r'GATC_y

Cuts at:      0     505    1049    1733    1954    2426    3111    3312    3480 
   Size:        505     544     684     221     472     685     201     168

Cuts at:   3480    3944    4128 
   Size:        464     184

  Fragments arranged by size: 

                 685     684     544     505     472     464     221     201
                 184     168
 
BtgI C'CryG_G

Cuts at:      0     765    1577    3002    3185    4128 
   Size:        765     812    1425     183     943

  Fragments arranged by size: 

                1425     943     812     765     183
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     700    4128 
   Size:        700    3428
 
BtsI GCAGTG_nn'

Cuts at:      0    1645    1711    2886    3784    4128 
   Size:       1645      66    1175     898     344

  Fragments arranged by size: 

                1645    1175     898     344      66
 
DraI TTT'AAA

Cuts at:      0     677    4128 
   Size:        677    3451
 
DraIII CAC_nnn'GTG

Cuts at:      0     124     279    4128 
   Size:        124     155    3849

  Fragments arranged by size: 

                3849     155     124
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     641    4128 
   Size:        641    3487
 
EaeI y'GGCC_r

Cuts at:      0     297     521     872     884    1091    2628    2999    3191 
   Size:        297     224     351      12     207    1537     371     192

Cuts at:   3191    3710    3747    4010    4128 
   Size:        519      37     263     118

  Fragments arranged by size: 

                1537     519     371     351     297     263     224     207
                 192     118      37      12
 
EagI C'GGCC_G

Cuts at:      0     297     521     884    1091    2628    2999    3747    4128 
   Size:        297     224     363     207    1537     371     748     381

  Fragments arranged by size: 

                1537     748     381     371     363     297     224     207
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1294    1789    2639    3016    3946    4128 
   Size:       1294     495     850     377     930     182

  Fragments arranged by size: 

                1294     930     850     495     377     182
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2515    4128 
   Size:       2515    1613
 
EcoICRI GAG'CTC

Cuts at:      0     731    1337    1550    4128 
   Size:        731     606     213    2578

  Fragments arranged by size: 

                2578     731     606     213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     308     379     760     884     885    2059    2488 
   Size:        200     108      71     381     124       1    1174     429

Cuts at:   2488    2568    3375    3683    3819    4104    4128 
   Size:         80     807     308     136     285      24

  Fragments arranged by size: 

                1174     807     429     381     308     285     200     136
                 124     108      80      71      24       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     976    2158    2839    3320    3925    4128 
   Size:        976    1182     681     481     605     203

  Fragments arranged by size: 

                1182     976     681     605     481     203
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1311    1421    1488    2163    2294    2538    2723    3075 
   Size:       1311     110      67     675     131     244     185     352

Cuts at:   3075    3890    4099    4128 
   Size:        815     209      29

  Fragments arranged by size: 

                1311     815     675     352     244     209     185     131
                 110      67      29
 
EcoRI G'AATT_C

Cuts at:      0    1120    2206    4128 
   Size:       1120    1086    1922

  Fragments arranged by size: 

                1922    1120    1086
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     915    2958    3114    4128 
   Size:        915    2043     156    1014

  Fragments arranged by size: 

                2043    1014     915     156
 
HaeII r_GCGC'y

Cuts at:      0     783     908     995    1608    2033    2109    2154    3266 
   Size:        783     125      87     613     425      76      45    1112

Cuts at:   3266    3737    3761    4128 
   Size:        471      24     367

  Fragments arranged by size: 

                1112     783     613     471     425     367     125      87
                  76      45      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     580     612    1648    1675    1680    1707    1955    1981 
   Size:        580      32    1036      27       5      27     248      26

Cuts at:   1981    1987    2013    2418    2450    2899    2931    2947    2979 
   Size:          6      26     405      32     449      32      16      32

Cuts at:   2979    3796    3828    4128 
   Size:        817      32     300

  Fragments arranged by size: 

                1036     817     580     449     405     300     248      32
                  32      32      32      32      27      27      26      26
                  16       6       5
 
HincII GTy'rAC

Cuts at:      0    2024    2346    4128 
   Size:       2024     322    1782

  Fragments arranged by size: 

                2024    1782     322
 
KasI G'GCGC_C

Cuts at:      0     779     991    2029    2105    3262    4128 
   Size:        779     212    1038      76    1157     866

  Fragments arranged by size: 

                1157    1038     866     779     212      76
 
KpnI G_GTAC'C

Cuts at:      0    4118    4128 
   Size:       4118      10
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     522     758     770    2819    4128 
   Size:        522     236      12    2049    1309

  Fragments arranged by size: 

                2049    1309     522     236      12
 
MscI TGG'CCA

Cuts at:      0    3193    3712    4128 
   Size:       3193     519     416

  Fragments arranged by size: 

                3193     519     416
 
MslI CAynn'nnrTG

Cuts at:      0     233     836    1850    2585    2774    3172    4128 
   Size:        233     603    1014     735     189     398     956

  Fragments arranged by size: 

                1014     956     735     603     398     233     189
 
MspA1I CmG'CkG

Cuts at:      0     134     767     846    1271    1782    2132    2421    2478 
   Size:        134     633      79     425     511     350     289      57

Cuts at:   2478    3004    3291    3376    3703    3887    4128 
   Size:        526     287      85     327     184     241

  Fragments arranged by size: 

                 633     526     511     425     350     327     289     287
                 241     184     134      85      79      57
 
NaeI GCC'GGC

Cuts at:      0      90    2104    4128 
   Size:         90    2014    2024

  Fragments arranged by size: 

                2024    2014      90
 
NarI GG'CG_CC

Cuts at:      0     780     992    2030    2106    3263    4128 
   Size:        780     212    1038      76    1157     865

  Fragments arranged by size: 

                1157    1038     865     780     212      76
 
NgoMIV G'CCGG_C

Cuts at:      0      88    2102    4128 
   Size:         88    2014    2026

  Fragments arranged by size: 

                2026    2014      88
 
NheI G'CTAG_C

Cuts at:      0    2367    4128 
   Size:       2367    1761
 
NotI GC'GGCC_GC

Cuts at:      0    2999    4128 
   Size:       2999    1129
 
NsiI A_TGCA'T

Cuts at:      0    2494    4128 
   Size:       2494    1634
 
NspI r_CATG'y

Cuts at:      0     579     777    1200    2406    2773    4128 
   Size:        579     198     423    1206     367    1355

  Fragments arranged by size: 

                1355    1206     579     423     367     198
 
PasI CC'CwG_GG

Cuts at:      0    1400    4128 
   Size:       1400    2728
 
PciI A'CATG_T

Cuts at:      0    2402    4128 
   Size:       2402    1726
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      37     810    3993    4128 
   Size:         37     773    3183     135

  Fragments arranged by size: 

                3183     773     135      37
 
PfoI T'CCnGG_A

Cuts at:      0    1628    1807    2522    2903    3791    4128 
   Size:       1628     179     715     381     888     337

  Fragments arranged by size: 

                1628     888     715     381     337     179
 
PmlI CAC'GTG

Cuts at:      0    1014    1623    4008    4128 
   Size:       1014     609    2385     120

  Fragments arranged by size: 

                2385    1014     609     120
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3674    3706    4128 
   Size:       3674      32     422

  Fragments arranged by size: 

                3674     422      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1311    1488    2163    2538    2723    3075    4099    4128 
   Size:       1311     177     675     375     185     352    1024      29

  Fragments arranged by size: 

                1311    1024     675     375     352     185     177      29
            
 
PspOMI G'GGCC_C

Cuts at:      0    1202    1420    2294    3889    4128 
   Size:       1202     218     874    1595     239

  Fragments arranged by size: 

                1595    1202     874     239     218
 
PspXI vC'TCGA_Gb

Cuts at:      0    1338    4128 
   Size:       1338    2790
 
PstI C_TGCA'G

Cuts at:      0    1151    2162    2326    2426    2851    4128 
   Size:       1151    1011     164     100     425    1277

  Fragments arranged by size: 

                1277    1151    1011     425     164     100
 
PvuII CAG'CTG

Cuts at:      0     134     846    2132    2421    3376    3703    4128 
   Size:        134     712    1286     289     955     327     425

  Fragments arranged by size: 

                1286     955     712     425     327     289     134
 
SacI G_AGCT'C

Cuts at:      0     733    1339    1552    4128 
   Size:        733     606     213    2576

  Fragments arranged by size: 

                2576     733     606     213
 
SacII CC_GC'GG

Cuts at:      0     768    3005    4128 
   Size:        768    2237    1123

  Fragments arranged by size: 

                2237    1123     768
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1294    2639    4128 
   Size:       1294    1345    1489

  Fragments arranged by size: 

                1489    1345    1294
 
SbfI CC_TGCA'GG

Cuts at:      0    2851    4128 
   Size:       2851    1277
 
SexAI A'CCwGG_T

Cuts at:      0    3638    4110    4128 
   Size:       3638     472      18

  Fragments arranged by size: 

                3638     472      18
 
SfcI C'TryA_G

Cuts at:      0    1147    1749    2158    2289    2322    2422    2847    4128 
   Size:       1147     602     409     131      33     100     425    1281

  Fragments arranged by size: 

                1281    1147     602     425     409     131     100      33
            
 
SfoI GGC'GCC

Cuts at:      0     781     993    2031    2107    3264    4128 
   Size:        781     212    1038      76    1157     864

  Fragments arranged by size: 

                1157    1038     864     781     212      76
 
SmaI CCC'GGG

Cuts at:      0      79    2299    3824    3870    4128 
   Size:         79    2220    1525      46     258

  Fragments arranged by size: 

                2220    1525     258      79      46
 
SmlI C'TyrA_G

Cuts at:      0    1338    1542    1993    3142    4128 
   Size:       1338     204     451    1149     986

  Fragments arranged by size: 

                1338    1149     986     451     204
 
SphI G_CATG'C

Cuts at:      0     777    1200    2773    4128 
   Size:        777     423    1573    1355

  Fragments arranged by size: 

                1573    1355     777     423
 
SrfI GCCC'GGGC

Cuts at:      0    3824    4128 
   Size:       3824     304
 
SspI AAT'ATT

Cuts at:      0    2248    4128 
   Size:       2248    1880
 
StuI AGG'CCT

Cuts at:      0      19    1816    2239    2783    2845    4128 
   Size:         19    1797     423     544      62    1283

  Fragments arranged by size: 

                1797    1283     544     423      62      19
 
StyI C'CwwG_G

Cuts at:      0      52    1056    1503    3258    3713    4128 
   Size:         52    1004     447    1755     455     415

  Fragments arranged by size: 

                1755    1004     455     447     415      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1168    1318    1369    1570    2411    4128 
   Size:       1168     150      51     201     841    1717

  Fragments arranged by size: 

                1717    1168     841     201     150      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1624    1656    4128 
   Size:       1624      32    2472

  Fragments arranged by size: 

                2472    1624      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2047    2167    2377    4128 
   Size:       2047     120     210    1751

  Fragments arranged by size: 

                2047    1751     210     120
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1113    3201    4128 
   Size:       1113    2088     927

  Fragments arranged by size: 

                2088    1113     927
 
XhoI C'TCGA_G

Cuts at:      0    1338    4128 
   Size:       1338    2790
 
XmaI C'CCGG_G

Cuts at:      0      77    2297    3822    3868    4128 
   Size:         77    2220    1525      46     260

  Fragments arranged by size: 

                2220    1525     260      77      46
 
XmnI GAAnn'nnTTC

Cuts at:      0    2206    4128 
   Size:       2206    1922
 
ZraI GAC'GTC

Cuts at:      0    2379    4128 
   Size:       2379    1749
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI
    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI    SacII     SapI
     SbfI    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     SrfI
     SspI     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI    AflII     AscI    AsiSI     BaeI    BbvCI
     BcgI    BciVI     BclI    BsaBI    BsiWI    BsrGI   BssHII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     MfeI     MluI     NcoI     NdeI     NruI     PacI
     PmeI    PshAI     PsiI     PsrI     PvuI    RsrII     SalI    SanDI
     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SwaI     TatI
     XbaI
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