Restriction map for pF1KB5551
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AvrII       BamHI       BbsI        BbvCI       BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaBI       BseAI       BsiWI       BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     ClaI        CpoI        DraI        
DraIII      Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
SspI        StuI        SwaI        Tru9I       XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        1036  GACGTC               GACGT^C
AccI         1816  GTCTAC               GT^[AC][GT]AC
AcsI          176  AAATTC               [GA]^AATT[CT]
AflIII       2030  ACGTGT               A^C[GA][CT]GT
AhdI         1306  GACTGCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1414  GTGCAC               G^TGCAC
AlwI          492  GGATC                GGATC(4/5)
AocI          349  CCTGAGG              CC^T[ACGT]AGG
ApaI         1602  GGGCCC               GGGCC^C
ApaLI        1414  GTGCAC               G^TGCAC
ApoI          176  AAATTC               [GA]^AATT[CT]
AspEI        1306  GACTGCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1036  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          595  GGCGCC               GGCGC^C
BclI            2  TGATCA               T^GATCA
BlpI         1951  GCTGAGC              GC^T[ACGT]AGC
BpmI          583  CTGGAG               CTGGAG(16/14)
Bpu1102I     1951  GCTGAGC              GC^T[ACGT]AGC
BsaWI         875  ACCGGA               [AT]^CCGG[AT]
BsgI         1924  GTGCAG               GTGCAG(16/14)
BsmBI        1684  GAGACG               CGTCTC(1/5)
BsmI          731  GCATTC               GAATGC(1/-1)
Bsp1407I      782  TGTACA               T^GTACA
BspMI        1829  ACCTGC               ACCTGC(4/8)
BsrGI         782  TGTACA               T^GTACA
BstAPI       1203  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         492  GGATCT               [GA]^GATC[CT]
Bsu36I        349  CCTGAGG              CC^T[ACGT]AGG
CelII        1951  GCTGAGC              GC^T[ACGT]AGC
DraII        1602  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DrdI         1030  GACCTGGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1306  GACTGCCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        349  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1602  GGGCCCC              [GA]G^G[ACGT]CC[CT]
FbaI            2  TGATCA               T^GATCA
HincII       1000  GTCAAC               GT[CT]^[GA]AC
HindII       1000  GTCAAC               GT[CT]^[GA]AC
KasI          595  GGCGCC               G^GCGCC
MaeIII        865  GTCAC                ^GT[ACGT]AC
MflI          492  GGATCT               [GA]^GATC[CT]
NarI          595  GGCGCC               GG^CGCC
NspI          335  GCATGT               [GA]CATG^[CT]
PflFI        1036  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1478  CCACCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        1006  CAGCTG               CAG^CTG
SacI         1954  GAGCTC               GAGCT^C
SapI          807  GAAGAGC              GCTCTTC(1/4)
SfoI          595  GGCGCC               GGC^GCC
SmaI         1796  CCCGGG               CCC^GGG
SspBI         782  TGTACA               T^GTACA
Tsp45I        865  GTCAC                ^GT[GC]AC
TspRI         714  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I      1036  GACGTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1478  CCACCAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          776  CCAAGGTGTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         492  GGATCT               [GA]^GATC[CT]
XmaCI        1796  CCCGGG               C^CCGGG
XmaI         1796  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI        1204  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1825  CAGCACCTG           
Asp700I       846  GAATCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1929  GAACACCTTC          
AviII        1214  TGCGCA               TGC^GCA
             1547  TGCGCA              
BbrPI          22  CACGTG               CAC^GTG
              964  CACGTG              
BcgI          762  CGACATAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1869  CGAAGGCGGTGC        
BsrBI        1710  CCGCTC               CCGCTC(-3/-3)
             1645  GAGCGG              
DsaI          557  CCATGG               C^C[GA][CT]GG
             1964  CCATGG              
EarI          136  CTCTTC               CTCTTC(1/4)
              807  GAAGAG              
EcoNI         300  CCTGTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1059  CCTGGATGAGG         
FseI          680  GGCCGGCC             GGCCGG^CC
             1452  GGCCGGCC            
FspI         1214  TGCGCA               TGC^GCA
             1547  TGCGCA              
HaeII         595  GGCGCC               [GA]GCGC^[CT]
              899  AGCGCC              
HgaI          310  GACGC                GACGC(5/10)
             1686  GACGC               
Ksp632I       136  CTCTTC               CTCTTC(1/4)
              807  GAAGAG              
MspA1I        341  CAGCGG               C[AC]G^C[GT]G
             1006  CAGCTG              
NcoI          557  CCATGG               C^CATGG
             1964  CCATGG              
PmaCI          22  CACGTG               CAC^GTG
              964  CACGTG              
PmlI           22  CACGTG               CAC^GTG
              964  CACGTG              
SbfI         1299  CCTGCAGG             CCTGCA^GG
             1359  CCTGCAGG            
Sse8387I     1299  CCTGCAGG             CCTGCA^GG
             1359  CCTGCAGG            
Tsp509I       177  AATT                 ^AATT
              920  AATT                
XmnI          846  GAATCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1929  GAACACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BmyI        BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       EaeI        
EagI        EclXI       Eco52I      EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MboI        MboII       
MluNI       MnlI        MscI        MslI        MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
TseI        TthHB8I     
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