Restriction map for pF1KB5551
 (Linear) (Six-base) MAPSORT of: KIBB5551.seq  Check: 8291  from: 1  to: 2040



KIBB5551.orf

 With 182 enzymes: SgfI * 

                        May 19, 2017 16:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1838    2040 
   Size:       1838     202
 
AatII G_ACGT'C

Cuts at:      0    1040    2040 
   Size:       1040    1000
 
AccI GT'mk_AC

Cuts at:      0    1817    2040 
   Size:       1817     223
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     826    2040 
   Size:        123     703    1214

  Fragments arranged by size: 

                1214     703     123
 
AflIII A'CryG_T

Cuts at:      0    2030    2040 
   Size:       2030      10
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1311    2040 
   Size:       1311     729
 
AleI CACnn'nnGTG

Cuts at:      0    1340    1421    1615    2040 
   Size:       1340      81     194     425

  Fragments arranged by size: 

                1340     425     194      81
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     569    1439    2040 
   Size:        569     870     601

  Fragments arranged by size: 

                 870     601     569
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1209    1830    2040 
   Size:       1209     621     210

  Fragments arranged by size: 

                1209     621     210
 
ApaI G_GGCC'C

Cuts at:      0    1606    2040 
   Size:       1606     434
 
ApaLI G'TGCA_C

Cuts at:      0    1414    2040 
   Size:       1414     626
 
ApoI r'AATT_y

Cuts at:      0     176    2040 
   Size:        176    1864
 
AvaI C'yCGr_G

Cuts at:      0     163    1796    1957    2040 
   Size:        163    1633     161      83

  Fragments arranged by size: 

                1633     163     161      83
 
BanI G'GyrC_C

Cuts at:      0     595    1024    1533    1609    1620    1876    2040 
   Size:        595     429     509      76      11     256     164

  Fragments arranged by size: 

                 595     509     429     256     164      76      11
 
BanII G_rGCy'C

Cuts at:      0    1196    1606    1958    2040 
   Size:       1196     410     352      82

  Fragments arranged by size: 

                1196     410     352      82
 
BbeI G_GCGC'C

Cuts at:      0     599    2040 
   Size:        599    1441
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     751     785    1858    1892    2040 
   Size:        751      34    1073      34     148

  Fragments arranged by size: 

                1073     751     148      34      34
 
BclI T'GATC_A

Cuts at:      0       2    2040 
   Size:          2    2038
 
BlpI GC'TnA_GC

Cuts at:      0    1952    2040 
   Size:       1952      88
 
Bme1580I G_kGCm'C

Cuts at:      0    1099    1418    1538    1606    1881    2040 
   Size:       1099     319     120      68     275     159

  Fragments arranged by size: 

                1099     319     275     159     120      68
 
BmgBI CAC'GTC

Cuts at:      0     489    2031    2040 
   Size:        489    1542       9

  Fragments arranged by size: 

                1542     489       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1551    2040 
   Size:       1551     489
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604    2040 
   Size:        604    1436
 
Bpu10I CC'TnA_GC

Cuts at:      0     373    1258    2040 
   Size:        373     885     782

  Fragments arranged by size: 

                 885     782     373
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     121     431    1438    2040 
   Size:        121     310    1007     602

  Fragments arranged by size: 

                1007     602     310     121
 
BsaAI yAC'GTr

Cuts at:      0      24     570     966    2040 
   Size:         24     546     396    1074

  Fragments arranged by size: 

                1074     546     396      24
 
BsaHI Gr'CG_yC

Cuts at:      0     311     596    1037    2040 
   Size:        311     285     441    1003

  Fragments arranged by size: 

                1003     441     311     285
 
BsaWI w'CCGG_w

Cuts at:      0     875    2040 
   Size:        875    1165
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      19      49    2040 
   Size:         19      30    1991

  Fragments arranged by size: 

                1991      30      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     183     298     367     661     736    1081    1218    1221 
   Size:        183     115      69     294      75     345     137       3

Cuts at:   1221    1753    2040 
   Size:        532     287

  Fragments arranged by size: 

                 532     345     294     287     183     137     115      75
                  69       3
 
BseYI C'CCAG_C

Cuts at:      0     599     615     978    1008    1262    1775    2040 
   Size:        599      16     363      30     254     513     265

  Fragments arranged by size: 

                 599     513     363     265     254      30      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1945    2040 
   Size:       1945      95
 
BsiEI CG_ry'CG

Cuts at:      0     686    1458    1524    2040 
   Size:        686     772      66     516

  Fragments arranged by size: 

                 772     686     516      66
 
BsiHKAI G_wGCw'C

Cuts at:      0     548    1418    1853    1958    1973    2040 
   Size:        548     870     435     105      15      67

  Fragments arranged by size: 

                 870     548     435     105      67      15
 
BsmI GAATG_Cn'

Cuts at:      0     731    2040 
   Size:        731    1309
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1678    2040 
   Size:       1678     362
 
Bsp1286I G_dGCh'C

Cuts at:      0     548    1099    1196    1418    1538    1606    1853    1881 
   Size:        548     551      97     222     120      68     247      28

Cuts at:   1881    1958    1973    2040 
   Size:         77      15      67

  Fragments arranged by size: 

                 551     548     247     222     120      97      77      68
                  67      28      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1838    2040 
   Size:       1838     202
 
BsrBI CCG'CTC

Cuts at:      0    1647    1712    2040 
   Size:       1647      65     328

  Fragments arranged by size: 

                1647     328      65
 
BsrFI r'CCGG_y

Cuts at:      0     681     800    1453    2040 
   Size:        681     119     653     587

  Fragments arranged by size: 

                 681     653     587     119
 
BsrGI T'GTAC_A

Cuts at:      0     782    2040 
   Size:        782    1258
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1209    2040 
   Size:       1209     831
 
BstYI r'GATC_y

Cuts at:      0     492    2040 
   Size:        492    1548
 
Bsu36I CC'TnA_GG

Cuts at:      0     350    2040 
   Size:        350    1690
 
BtgI C'CryG_G

Cuts at:      0     557    1964    2040 
   Size:        557    1407      76

  Fragments arranged by size: 

                1407     557      76
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1365    2040 
   Size:       1365     675
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1036    2040 
   Size:       1036    1004
 
EaeI y'GGCC_r

Cuts at:      0      56     365     419     679     683     773    1292    1455 
   Size:         56     309      54     260       4      90     519     163

Cuts at:   1455    1475    1521    1913    2040 
   Size:         20      46     392     127

  Fragments arranged by size: 

                 519     392     309     260     163     127      90      56
                  54      46      20       4
 
EagI C'GGCC_G

Cuts at:      0     683    1455    1521    2040 
   Size:        683     772      66     519

  Fragments arranged by size: 

                 772     683     519      66
 
EarI CTCTTCn'nnn_

Cuts at:      0     142     802    2040 
   Size:        142     660    1238

  Fragments arranged by size: 

                1238     660     142
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1552    2040 
   Size:       1552     488
 
EcoICRI GAG'CTC

Cuts at:      0    1956    2040 
   Size:       1956      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     604     826    2040 
   Size:        123     481     222    1214

  Fragments arranged by size: 

                1214     481     222     123
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     304    1063    2040 
   Size:        304     759     977

  Fragments arranged by size: 

                 977     759     304
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1603    2040 
   Size:       1603     437
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      88     849    2040 
   Size:         88     761    1191

  Fragments arranged by size: 

                1191     761      88
 
FseI GG_CCGG'CC

Cuts at:      0     685    1457    2040 
   Size:        685     772     583

  Fragments arranged by size: 

                 772     685     583
 
FspI TGC'GCA

Cuts at:      0    1216    1549    2040 
   Size:       1216     333     491

  Fragments arranged by size: 

                1216     491     333
 
HaeII r_GCGC'y

Cuts at:      0     599     903    2040 
   Size:        599     304    1137

  Fragments arranged by size: 

                1137     599     304
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      19      51    1054    1086    1297    1329    2040 
   Size:         19      32    1003      32     211      32     711

  Fragments arranged by size: 

                1003     711     211      32      32      32      19
 
HincII GTy'rAC

Cuts at:      0    1002    2040 
   Size:       1002    1038
 
KasI G'GCGC_C

Cuts at:      0     595    2040 
   Size:        595    1445
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    2040 
   Size:       1097     943
 
MscI TGG'CCA

Cuts at:      0     421    1294    1477    1915    2040 
   Size:        421     873     183     438     125

  Fragments arranged by size: 

                 873     438     421     183     125
 
MslI CAynn'nnrTG

Cuts at:      0     250     551    1092    1340    1421    1543    1597    1615 
   Size:        250     301     541     248      81     122      54      18

Cuts at:   1615    2040 
   Size:        425

  Fragments arranged by size: 

                 541     425     301     250     248     122      81      54
                  18
 
MspA1I CmG'CkG

Cuts at:      0     343    1008    2040 
   Size:        343     665    1032

  Fragments arranged by size: 

                1032     665     343
 
NaeI GCC'GGC

Cuts at:      0     683     802    1455    2040 
   Size:        683     119     653     585

  Fragments arranged by size: 

                 683     653     585     119
 
NarI GG'CG_CC

Cuts at:      0     596    2040 
   Size:        596    1444
 
NcoI C'CATG_G

Cuts at:      0     557    1964    2040 
   Size:        557    1407      76

  Fragments arranged by size: 

                1407     557      76
 
NgoMIV G'CCGG_C

Cuts at:      0     681     800    1453    2040 
   Size:        681     119     653     587

  Fragments arranged by size: 

                 681     653     587     119
 
NspI r_CATG'y

Cuts at:      0     339    2040 
   Size:        339    1701
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1484    2040 
   Size:       1484     556
 
PfoI T'CCnGG_A

Cuts at:      0    1640    2040 
   Size:       1640     400
 
PmlI CAC'GTG

Cuts at:      0      24     966    2040 
   Size:         24     942    1074

  Fragments arranged by size: 

                1074     942      24
 
PspOMI G'GGCC_C

Cuts at:      0    1602    2040 
   Size:       1602     438
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     523     555    1921    1953    2040 
   Size:        523      32    1366      32      87

  Fragments arranged by size: 

                1366     523      87      32      32
 
PstI C_TGCA'G

Cuts at:      0     282     479     864    1304    1364    2040 
   Size:        282     197     385     440      60     676

  Fragments arranged by size: 

                 676     440     385     282     197      60
 
PvuII CAG'CTG

Cuts at:      0    1008    2040 
   Size:       1008    1032
 
SacI G_AGCT'C

Cuts at:      0    1958    2040 
   Size:       1958      82
 
SapI GCTCTTCn'nnn_

Cuts at:      0     802    2040 
   Size:        802    1238
 
SbfI CC_TGCA'GG

Cuts at:      0    1304    1364    2040 
   Size:       1304      60     676

  Fragments arranged by size: 

                1304     676      60
 
SfcI C'TryA_G

Cuts at:      0     278     475     860    1300    1360    2040 
   Size:        278     197     385     440      60     680

  Fragments arranged by size: 

                 680     440     385     278     197      60
 
SfoI GGC'GCC

Cuts at:      0     597    2040 
   Size:        597    1443
 
SmaI CCC'GGG

Cuts at:      0    1798    2040 
   Size:       1798     242
 
StyI C'CwwG_G

Cuts at:      0     305     557     776    1394    1448    1496    1964    2009 
   Size:        305     252     219     618      54      48     468      45

Cuts at:   2009    2040 
   Size:         31

  Fragments arranged by size: 

                 618     468     305     252     219      54      48      45
                  31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1012    1100    1254    1789    1859    2040 
   Size:       1012      88     154     535      70     181

  Fragments arranged by size: 

                1012     535     181     154      88      70
 
TatI w'GTAC_w

Cuts at:      0     782    1694    2040 
   Size:        782     912     346

  Fragments arranged by size: 

                 912     782     346
 
Tth111I GACn'n_nGTC

Cuts at:      0    1039    2040 
   Size:       1039    1001
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     783    2040 
   Size:        783    1257
 
XmaI C'CCGG_G

Cuts at:      0    1796    2040 
   Size:       1796     244
 
XmnI GAAnn'nnTTC

Cuts at:      0     850    1933    2040 
   Size:        850    1083     107

  Fragments arranged by size: 

                1083     850     107
 
ZraI GAC'GTC

Cuts at:      0    1038    2040 
   Size:       1038    1002
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI
     BcgI     BclI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
    BsrGI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FseI     FspI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI    PflMI
     PfoI     PmlI   PspOMI     PsrI     PstI    PvuII     SacI     SapI
     SbfI     SfcI     SfoI     SmaI     StyI    TaqII     TatI  Tth111I
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI    BfrBI
     BglI    BglII     BmtI     BplI    BpuEI    BsaBI    BsiWI    BspEI
    BspHI    BsrDI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
     BtsI     ClaI    CspCI     DraI   DraIII    EcoRI    EcoRV    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI     XhoI
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