Restriction map for pF1KB5446
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BalI        
BbeI        BbrPI       BbsI        BciVI       BclI        
BfrI        BglI        BlnI        BpuAI       BsaAI       
BsaHI       BsgI        BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       FbaI        FseI        
FspI        Hin1I       HincII      HindII      HpaI        
KasI        KspI        MfeI        MluI        MluNI       
MscI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1048  GGTACC               G^GTACC
AccIII       1373  TCCGGA               T^CCGGA
AcsI         1115  AAATTC               [GA]^AATT[CT]
ApoI         1115  AAATTC               [GA]^AATT[CT]
Asp700I      1681  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1048  GGTACC               G^GTACC
AvaII        1212  GGACC                G^G[AT]CC
BamHI        1838  GGATCC               G^GATCC
BglII        1359  AGATCT               A^GATCT
BlpI          133  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      133  GCTCAGC              GC^T[ACGT]AGC
BsaBI         897  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          388  GAGACC               GGTCTC(1/5)
BsaWI        1373  TCCGGA               [AT]^CCGG[AT]
BseAI        1373  TCCGGA               T^CCGGA
BseRI         385  GAGGAG               GAGGAG(10/8)
BspEI        1373  TCCGGA               T^CCGGA
BspHI        1325  TCATGA               T^CATGA
BspMI        1045  GCAGGT               ACCTGC(4/8)
BsrDI        1065  CATTGC               GCAATG(2/0)
BsrFI         192  ACCGGC               [GA]^CCGG[CT]
BstEII       1941  GGTCACC              G^GT[ACGT]ACC
BstPI        1941  GGTCACC              G^GT[ACGT]ACC
CelII         133  GCTCAGC              GC^T[ACGT]AGC
CfoI           76  GCGC                 GCG^C
Cfr10I        192  ACCGGC               [GA]^CCGG[CT]
DraIII       1812  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          286  GAAGAG               CTCTTC(1/4)
EcoNI        1764  CCTGAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1941  GGTCACC              G^GT[ACGT]ACC
HaeII          75  GGCGCT               [GA]GCGC^[CT]
HhaI           76  GCGC                 GCG^C
HinP1I         76  GCGC                 G^CGC
HindIII       739  AAGCTT               A^AGCTT
KpnI         1048  GGTACC               GGTAC^C
Ksp632I       286  GAAGAG               CTCTTC(1/4)
MaeII         962  ACGT                 A^CGT
MamI          897  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1373  TCCGGA               T^CCGGA
MseI          305  TTAA                 T^TAA
NspI          503  GCATGC               [GA]CATG^[CT]
PaeR7I       1746  CTCGAG               C^TCGAG
PflMI        1302  CCAAATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1325  TCATGA               T^CATGA
SapI          286  GAAGAGC              GCTCTTC(1/4)
SexAI        1813  ACCTGGT              A^CC[AT]GGT
SphI          503  GCATGC               GCATG^C
StuI         1916  AGGCCT               AGG^CCT
Tru9I         305  TTAA                 T^TAA
Tsp509I      1116  AATT                 ^AATT
Van91I       1302  CCAAATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1746  CTCGAG               C^TCGAG
XmnI         1681  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII          21  CGCG                 CG^CG
               65  CGCG                
ApaI          536  GGGCCC               GGGCC^C
              880  GGGCCC              
AvaI           49  CTCGGG               C^[CT]CG[GA]G
             1746  CTCGAG              
BbvCI          42  GCTGAGG              CCTCAGC(-5/-2)
             1524  GCTGAGG             
BcgI         1719  CGACCTCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
                3  GCAGGCGGCTCG        
BfaI          763  CTAG                 C^TAG
             1504  CTAG                
BsmFI        1387  GGGAC                GGGAC(10/14)
              327  GTCCC               
BsmI         1294  GAATGC               GAATGC(1/-1)
             1488  GAATGC              
BsoBI          49  CTCGGG               C^[CT]CG[GA]G
             1746  CTCGAG              
BsrBI         131  CCGCTC               CCGCTC(-3/-3)
             1927  GAGCGG              
BstUI          21  CGCG                 CG^CG
               65  CGCG                
EcoT22I       505  ATGCAT               ATGCA^T
             1296  ATGCAT              
HgaI          172  GACGC                GACGC(5/10)
              367  GACGC               
MaeI          763  CTAG                 C^TAG
             1504  CTAG                
MslI         1632  CACTGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1731  CATGGTGGTG          
MvnI           21  CGCG                 CG^CG
               65  CGCG                
NsiI          505  ATGCAT               ATGCA^T
             1296  ATGCAT              
PvuII         136  CAGCTG               CAG^CTG
             1522  CAGCTG              
SacI         1447  GAGCTC               GAGCT^C
             1885  GAGCTC              
XcmI         1184  CCATGGCCTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1913  CCAAGGCCTTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     XhoII       
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