Restriction map for pF1KB5095
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AspEI       AvrII       BbeI        BbrPI       
BcgI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BspEI       BspMI       BsrBI       BssHII      
BssSI       BstBI       Bsu36I      CpoI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRV       FauI        FseI        
HaeII       Hin1I       HindIII     HpaI        KasI        
KspI        MamI        MluI        MroI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
StuI        SwaI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        130  GGTACC               G^GTACC
AccI         1519  GTATAC               GT^[AC][GT]AC
AccII          10  CGCG                 CG^CG
AciI            9  CCGC                 CCGC(-3/-1)
Alw44I       1219  GTGCAC               G^TGCAC
ApaLI        1219  GTGCAC               G^TGCAC
AseI         1842  ATTAAT               AT^TAAT
AsnI         1842  ATTAAT               AT^TAAT
Asp700I      1881  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       130  GGTACC               G^GTACC
AspHI        1219  GTGCAC               G[AT]GC[AT]^C
AspI         1594  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2324  TGCGCA               TGC^GCA
BalI         2642  TGGCCA               TGG^CCA
BanII         783  GAGCCC               G[GA]GC[CT]^C
BbvCI         787  CCTCAGC              CCTCAGC(-5/-2)
BclI         2567  TGATCA               T^GATCA
BglI         2789  GCCGTAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2823  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2823  GCTTAGC              GC^T[ACGT]AGC
BsaWI        2625  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      1219  GTGCAC               G[AT]GC[AT]^C
BsmBI        2710  GAGACG               CGTCTC(1/5)
BsmI         2169  GCATTC               GAATGC(1/-1)
BspDI        2441  ATCGAT               AT^CGAT
BspLU11I      308  ACATGT               A^CATGT
BsrDI        2646  CATTGC               GCAATG(2/0)
BsrFI        2819  ACCGGC               [GA]^CCGG[CT]
Bst1107I     1519  GTATAC               GTA^TAC
BstAPI       2541  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          10  CGCG                 CG^CG
BstZ17I      1519  GTATAC               GTA^TAC
CelII        2823  GCTTAGC              GC^T[ACGT]AGC
Cfr10I       2819  ACCGGC               [GA]^CCGG[CT]
ClaI         2441  ATCGAT               AT^CGAT
EcoT14I      2769  CCTTGG               C^C[AT][AT]GG
FbaI         2567  TGATCA               T^GATCA
FspI         2324  TGCGCA               TGC^GCA
HgaI           11  GCGTC                GACGC(5/10)
KpnI          130  GGTACC               GGTAC^C
MfeI         1658  CAATTG               C^AATTG
MluNI        2642  TGGCCA               TGG^CCA
MscI         2642  TGGCCA               TGG^CCA
MunI         1658  CAATTG               C^AATTG
MvnI           10  CGCG                 CG^CG
PflFI        1594  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1918  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1842  ATTAAT               AT^TAAT
PstI         1700  CTGCAG               CTGCA^G
SpeI         1389  ACTAGT               A^CTAGT
SspI          391  AATATT               AAT^ATT
StyI         2769  CCTTGG               C^C[AT][AT]GG
Tth111I      1594  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1918  CCAGGCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1554  TCTAGA               T^CTAGA
XcmI         1596  CCATGTCTGGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1881  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AflIII        308  ACATGT               A^C[GA][CT]GT
              565  ACACGT              
BanI          130  GGTACC               G^G[CT][GA]CC
             1456  GGCACC              
BbsI         1666  GAAGAC               GAAGAC(2/6)
             2016  GTCTTC              
BciVI         294  GGATAC               GTATCC(6/5)
              739  GGATAC              
BcnI          428  CCCGG                CC^[GC]GG
             2106  CCGGG               
BmyI          783  GAGCCC               G[AGT]GC[ACT]^C
             1219  GTGCAC              
BpuAI        1666  GAAGAC               GAAGAC(2/6)
             2016  GTCTTC              
BsgI         1328  GTGCAG               GTGCAG(16/14)
             1969  GTGCAG              
Bsp1286I      783  GAGCCC               G[AGT]GC[ACT]^C
             1219  GTGCAC              
BspHI         977  TCATGA               T^CATGA
             2346  TCATGA              
BstEII        841  GGTGACC              G^GT[ACGT]ACC
             1591  GGTGACC             
BstPI         841  GGTGACC              G^GT[ACGT]ACC
             1591  GGTGACC             
CfoI         2325  GCGC                 GCG^C
             2902  GCGC                
DraI           91  TTTAAA               TTT^AAA
             2131  TTTAAA              
DraII        1833  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             2677  AGGGCCT             
EaeI            6  TGGCCG               [CT]^GGCC[GA]
             2642  TGGCCA              
EcoO109I     1833  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             2677  AGGGCCT             
EcoO65I       841  GGTGACC              G^GT[ACGT]ACC
             1591  GGTGACC             
EcoRI         648  GAATTC               G^AATTC
             1370  GAATTC              
EcoT22I       685  ATGCAT               ATGCA^T
             1072  ATGCAT              
HhaI         2325  GCGC                 GCG^C
             2902  GCGC                
HinP1I       2325  GCGC                 G^CGC
             2902  GCGC                
HincII        613  GTTGAC               GT[CT]^[GA]AC
             1892  GTCAAC              
HindII        613  GTTGAC               GT[CT]^[GA]AC
             1892  GTCAAC              
MspA1I         48  CAGCTG               C[AC]G^C[GT]G
              790  CAGCTG              
NciI          428  CCCGG                CC^[GC]GG
             2106  CCGGG               
NsiI          685  ATGCAT               ATGCA^T
             1072  ATGCAT              
PaeR7I        281  CTCGAG               C^TCGAG
             2707  CTCGAG              
PvuII          48  CAGCTG               CAG^CTG
              790  CAGCTG              
RcaI          977  TCATGA               T^CATGA
             2346  TCATGA              
ScaI         1704  AGTACT               AGT^ACT
             2727  AGTACT              
XhoI          281  CTCGAG               C^TCGAG
             2707  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        AvaI        AvaII       BamHI       BbvI        
BfaI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1407I    
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraIII      EarI        EcoNI       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmlI        SphI        
SspBI       TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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