Restriction map for pF1KB5095
 (Linear) (Six-base) MAPSORT of: KIBB5095.seq  Check: 1586  from: 1  to: 2922



KIBB5095.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0    1520    2922 
   Size:       1520    1402
 
Acc65I G'GTAC_C

Cuts at:      0     130    2922 
   Size:        130    2792
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2003    2384    2922 
   Size:       2003     381     538

  Fragments arranged by size: 

                2003     538     381
 
AflIII A'CryG_T

Cuts at:      0     308     565    2922 
   Size:        308     257    2357

  Fragments arranged by size: 

                2357     308     257
 
AleI CACnn'nnGTG

Cuts at:      0     902    1589    1786    1903    2922 
   Size:        902     687     197     117    1019

  Fragments arranged by size: 

                1019     902     687     197     117
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     250     710    1984    2050    2922 
   Size:        250     460    1274      66     872

  Fragments arranged by size: 

                1274     872     460     250      66
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1924    2565    2695    2922 
   Size:       1924     641     130     227

  Fragments arranged by size: 

                1924     641     227     130
 
ApaLI G'TGCA_C

Cuts at:      0    1219    2922 
   Size:       1219    1703
 
ApoI r'AATT_y

Cuts at:      0     648    1370    1378    2149    2922 
   Size:        648     722       8     771     773

  Fragments arranged by size: 

                 773     771     722     648       8
 
AseI AT'TA_AT

Cuts at:      0    1843    2922 
   Size:       1843    1079
 
AvaI C'yCGr_G

Cuts at:      0     281    2583    2707    2922 
   Size:        281    2302     124     215

  Fragments arranged by size: 

                2302     281     215     124
 
BamHI G'GATC_C

Cuts at:      0    1815    1914    2120    2922 
   Size:       1815      99     206     802

  Fragments arranged by size: 

                1815     802     206      99
 
BanI G'GyrC_C

Cuts at:      0     130    1456    2922 
   Size:        130    1326    1466

  Fragments arranged by size: 

                1466    1326     130
 
BanII G_rGCy'C

Cuts at:      0     787    2922 
   Size:        787    2135
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1673    2009    2922 
   Size:       1673     336     913

  Fragments arranged by size: 

                1673     913     336
 
BbvCI CC'TCA_GC

Cuts at:      0     788    2922 
   Size:        788    2134
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     288     733    2922 
   Size:        288     445    2189

  Fragments arranged by size: 

                2189     445     288
 
BclI T'GATC_A

Cuts at:      0    2567    2922 
   Size:       2567     355
 
BfrBI ATG'CAT

Cuts at:      0     687    1074    2922 
   Size:        687     387    1848

  Fragments arranged by size: 

                1848     687     387
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2795    2922 
   Size:       2795     127
 
BlpI GC'TnA_GC

Cuts at:      0    2824    2922 
   Size:       2824      98
 
Bme1580I G_kGCm'C

Cuts at:      0    1223    2922 
   Size:       1223    1699
 
BmgBI CAC'GTC

Cuts at:      0     568     911    1788    2922 
   Size:        568     343     877    1134

  Fragments arranged by size: 

                1134     877     568     343
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2740    2922 
   Size:       2740     182
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     434    2048    2598    2922 
   Size:        434    1614     550     324

  Fragments arranged by size: 

                1614     550     434     324
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     986    1535    1693    1946    2922 
   Size:        986     549     158     253     976

  Fragments arranged by size: 

                 986     976     549     253     158
 
Bpu10I CC'TnA_GC

Cuts at:      0     788    2922 
   Size:        788    2134
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1422    1770    2922 
   Size:       1422     348    1152

  Fragments arranged by size: 

                1422    1152     348
 
BsaWI w'CCGG_w

Cuts at:      0    2625    2922 
   Size:       2625     297
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     626     656    2579    2609    2922 
   Size:        626      30    1923      30     313

  Fragments arranged by size: 

                1923     626     313      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     476    2520    2893    2907    2922 
   Size:        476    2044     373      14      15

  Fragments arranged by size: 

                2044     476     373      15      14
 
BseYI C'CCAG_C

Cuts at:      0     627    2922 
   Size:        627    2295
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1349    1990    2922 
   Size:       1349     641     932

  Fragments arranged by size: 

                1349     932     641
 
BsiHKAI G_wGCw'C

Cuts at:      0    1223    2922 
   Size:       1223    1699
 
BsmI GAATG_Cn'

Cuts at:      0    2169    2922 
   Size:       2169     753
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2704    2922 
   Size:       2704     218
 
Bsp1286I G_dGCh'C

Cuts at:      0     787    1223    2922 
   Size:        787     436    1699

  Fragments arranged by size: 

                1699     787     436
 
BspHI T'CATG_A

Cuts at:      0     977    2346    2922 
   Size:        977    1369     576

  Fragments arranged by size: 

                1369     977     576
 
BsrDI GCAATG_nn'

Cuts at:      0    2645    2922 
   Size:       2645     277
 
BsrFI r'CCGG_y

Cuts at:      0    2819    2922 
   Size:       2819     103
 
BsrGI T'GTAC_A

Cuts at:      0     376    2265    2378    2922 
   Size:        376    1889     113     544

  Fragments arranged by size: 

                1889     544     376     113
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2547    2922 
   Size:       2547     375
 
BstEII G'GTnAC_C

Cuts at:      0     841    1591    2922 
   Size:        841     750    1331

  Fragments arranged by size: 

                1331     841     750
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1543    1717    1905    2922 
   Size:       1543     174     188    1017

  Fragments arranged by size: 

                1543    1017     188     174
 
BstYI r'GATC_y

Cuts at:      0    1299    1686    1815    1914    2120    2922 
   Size:       1299     387     129      99     206     802

  Fragments arranged by size: 

                1299     802     387     206     129      99
 
BstZ17I GTA'TAC

Cuts at:      0    1521    2922 
   Size:       1521    1401
 
BtsI GCAGTG_nn'

Cuts at:      0    1878    2234    2844    2922 
   Size:       1878     356     610      78

  Fragments arranged by size: 

                1878     610     356      78
 
ClaI AT'CG_AT

Cuts at:      0    2442    2922 
   Size:       2442     480
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     882     917    1730    1765    2922 
   Size:        882      35     813      35    1157

  Fragments arranged by size: 

                1157     882     813      35      35
 
DraI TTT'AAA

Cuts at:      0      93    2133    2922 
   Size:         93    2040     789

  Fragments arranged by size: 

                2040     789      93
 
DraIII CAC_nnn'GTG

Cuts at:      0    1450    1984    2003    2408    2922 
   Size:       1450     534      19     405     514

  Fragments arranged by size: 

                1450     534     514     405      19
 
EaeI y'GGCC_r

Cuts at:      0       6    2642    2922 
   Size:          6    2636     280

  Fragments arranged by size: 

                2636     280       6
 
EarI CTCTTCn'nnn_

Cuts at:      0    1490    2006    2718    2870    2922 
   Size:       1490     516     712     152      52

  Fragments arranged by size: 

                1490     712     516     152      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     986    1535    1693    1946    2003    2384    2922 
   Size:        986     549     158     253      57     381     538

  Fragments arranged by size: 

                 986     549     538     381     253     158      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1451    2036    2267    2922 
   Size:       1451     585     231     655

  Fragments arranged by size: 

                1451     655     585     231
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1834    2678    2922 
   Size:       1834     844     244

  Fragments arranged by size: 

                1834     844     244
 
EcoRI G'AATT_C

Cuts at:      0     648    1370    2922 
   Size:        648     722    1552

  Fragments arranged by size: 

                1552     722     648
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2436    2922 
   Size:       2436     486
 
FspI TGC'GCA

Cuts at:      0    2326    2922 
   Size:       2326     596
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1597    1629    2922 
   Size:       1597      32    1293

  Fragments arranged by size: 

                1597    1293      32
 
HincII GTy'rAC

Cuts at:      0     615    1894    2922 
   Size:        615    1279    1028

  Fragments arranged by size: 

                1279    1028     615
 
KpnI G_GTAC'C

Cuts at:      0     134    2922 
   Size:        134    2788
 
MfeI C'AATT_G

Cuts at:      0    1658    2922 
   Size:       1658    1264
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     426     438    2098    2922 
   Size:        426      12    1660     824

  Fragments arranged by size: 

                1660     824     426      12
 
MscI TGG'CCA

Cuts at:      0    2644    2922 
   Size:       2644     278
 
MslI CAynn'nnrTG

Cuts at:      0     232     902    1589    1786    1903    2922 
   Size:        232     670     687     197     117    1019

  Fragments arranged by size: 

                1019     687     670     232     197     117
 
MspA1I CmG'CkG

Cuts at:      0      50     792    2922 
   Size:         50     742    2130

  Fragments arranged by size: 

                2130     742      50
 
NsiI A_TGCA'T

Cuts at:      0     689    1076    2922 
   Size:        689     387    1846

  Fragments arranged by size: 

                1846     689     387
 
NspI r_CATG'y

Cuts at:      0     189     231     312     597     691     967    1863    2922 
   Size:        189      42      81     285      94     276     896    1059

  Fragments arranged by size: 

                1059     896     285     276     189      94      81      42
            
 
PciI A'CATG_T

Cuts at:      0     308    2922 
   Size:        308    2614
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1924    2922 
   Size:       1924     998
 
PfoI T'CCnGG_A

Cuts at:      0    2471    2922 
   Size:       2471     451
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2690    2722    2922 
   Size:       2690      32     200

  Fragments arranged by size: 

                2690     200      32
 
PsiI TTA'TAA

Cuts at:      0     671    2922 
   Size:        671    2251
 
PspXI vC'TCGA_Gb

Cuts at:      0     281    2922 
   Size:        281    2641
 
PstI C_TGCA'G

Cuts at:      0    1704    2922 
   Size:       1704    1218
 
PvuII CAG'CTG

Cuts at:      0      50     792    2922 
   Size:         50     742    2130

  Fragments arranged by size: 

                2130     742      50
 
ScaI AGT'ACT

Cuts at:      0    1706    2729    2922 
   Size:       1706    1023     193

  Fragments arranged by size: 

                1706    1023     193
 
SexAI A'CCwGG_T

Cuts at:      0    1262    1446    1571    2922 
   Size:       1262     184     125    1351

  Fragments arranged by size: 

                1351    1262     184     125
 
SfcI C'TryA_G

Cuts at:      0     490     699    1120    1235    1700    2799    2922 
   Size:        490     209     421     115     465    1099     123

  Fragments arranged by size: 

                1099     490     465     421     209     123     115
 
SmlI C'TyrA_G

Cuts at:      0     281    1401    1749    2707    2922 
   Size:        281    1120     348     958     215

  Fragments arranged by size: 

                1120     958     348     281     215
 
SpeI A'CTAG_T

Cuts at:      0    1389    2922 
   Size:       1389    1533
 
SphI G_CATG'C

Cuts at:      0     189     967    1863    2922 
   Size:        189     778     896    1059

  Fragments arranged by size: 

                1059     896     778     189
 
SspI AAT'ATT

Cuts at:      0     393    2922 
   Size:        393    2529
 
StyI C'CwwG_G

Cuts at:      0    2769    2922 
   Size:       2769     153
 
TatI w'GTAC_w

Cuts at:      0     376     486    1704    2265    2378    2727    2922 
   Size:        376     110    1218     561     113     349     195

  Fragments arranged by size: 

                1218     561     376     349     195     113     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     626     658    2922 
   Size:        626      32    2264

  Fragments arranged by size: 

                2264     626      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1597    2922 
   Size:       1597    1325
 
XbaI T'CTAG_A

Cuts at:      0    1554    2922 
   Size:       1554    1368
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1603    2922 
   Size:       1603    1319
 
XhoI C'TCGA_G

Cuts at:      0     281    2707    2922 
   Size:        281    2426     215

  Fragments arranged by size: 

                2426     281     215
 
XmnI GAAnn'nnTTC

Cuts at:      0    1885    2922 
   Size:       1885    1037
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrDI    BsrFI
    BsrGI   BstAPI   BstEII    BstXI    BstYI  BstZ17I     BtsI     ClaI
    CspCI     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    Hin4I   HincII     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NsiI     NspI     PciI    PflMI     PfoI
     PpiI     PsiI    PspXI     PstI    PvuII     ScaI    SexAI     SfcI
     SmlI     SpeI     SphI     SspI     StyI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI     AscI    AsiSI    AvrII     BaeI     BbeI     BcgI    BglII
     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI    BspEI
    BspMI    BsrBI   BssHII    BssSI    BstBI   Bsu36I     BtgI    BtgZI
     DrdI     EagI     EciI  EcoICRI    EcoRV     FseI    FspAI    HaeII
  HindIII     HpaI     KasI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI
    PpuMI    PshAI   PspOMI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SrfI     StuI     SwaI    TaqII     XmaI     ZraI
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