Restriction map for pF1KB3518
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AocI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BlnI        BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstAPI      BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SapI        ScaI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tsp509I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        1174  GACGTC               GACGT^C
AfaI          540  GTAC                 GT^AC
AhdI          577  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       764  AGCGCT               AGC^GCT
AspEI         577  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         2264  TGGCCA               TGG^CCA
BglI          655  GCCCCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1230  AGATCT               A^GATCT
BlpI         1626  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1626  GCTGAGC              GC^T[ACGT]AGC
BsaI           99  GGTCTC               GGTCTC(1/5)
BsmBI        2131  CGTCTC               CGTCTC(1/5)
BsrDI        2238  CATTGC               GCAATG(2/0)
BsrFI        2031  GCCGGT               [GA]^CCGG[CT]
BstBI         235  TTCGAA               TT^CGAA
BstEII        483  GGTCACC              G^GT[ACGT]ACC
BstPI         483  GGTCACC              G^GT[ACGT]ACC
CelII        1626  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       2031  GCCGGT               [GA]^CCGG[CT]
DraIII       1834  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           17  CGGCCG               C^GGCCG
Eam1105I      577  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          547  GAAGAG               CTCTTC(1/4)
EclXI          17  CGGCCG               C^GGCCG
Eco47III      764  AGCGCT               AGC^GCT
Eco52I         17  CGGCCG               C^GGCCG
EcoO65I       483  GGTCACC              G^GT[ACGT]ACC
Ksp632I       547  GAAGAG               CTCTTC(1/4)
KspI           23  CCGCGG               CCGC^GG
MluNI        2264  TGGCCA               TGG^CCA
MscI         2264  TGGCCA               TGG^CCA
MseI         1292  TTAA                 T^TAA
MslI         1800  CACCTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         2042  CCATGG               C^CATGG
NotI           16  GCGGCCGC             GC^GGCCGC
NspV          235  TTCGAA               TT^CGAA
PvuII        1260  CAGCTG               CAG^CTG
RsaI          540  GTAC                 GT^AC
SacI          622  GAGCTC               GAGCT^C
SacII          23  CCGCGG               CCGC^GG
SbfI          676  CCTGCAGG             CCTGCA^GG
SexAI         338  ACCTGGT              A^CC[AT]GGT
SfuI          235  TTCGAA               TT^CGAA
SmlI         1405  CTCAAG               C^T[CT][GA]AG
Sse8387I      676  CCTGCAGG             CCTGCA^GG
Tru9I        1292  TTAA                 T^TAA
XcmI          464  CCAATGGCCCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI         1174  GACGTC               G[GA]^CG[CT]C
             1654  GACGCC              
Alw44I       1008  GTGCAC               G^TGCAC
             2035  GTGCAC              
ApaLI        1008  GTGCAC               G^TGCAC
             2035  GTGCAC              
AspI         1861  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2059  GACGCAGTC           
BamHI         200  GGATCC               G^GATCC
             1596  GGATCC              
BanI          897  GGTGCC               G^G[CT][GA]CC
             1717  GGCACC              
BbvCI        1257  CCTCAGC              CCTCAGC(-5/-2)
             1587  CCTCAGC             
BsaHI        1174  GACGTC               G[GA]^CG[CT]C
             1654  GACGCC              
BsiEI          17  CGGCCG               CG[GA][CT]^CG
               26  CGGTCG              
BssHII        229  GCGCGC               G^CGCGC
              267  GCGCGC              
BssSI         220  CACGAG               CACGAG(-5/-1)
             2191  CACGAG              
BstXI         887  CCAAGGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1799  CCACCTCAGTGG        
Hin1I        1174  GACGTC               G[GA]^CG[CT]C
             1654  GACGCC              
NspI          578  ACATGC               [GA]CATG^[CT]
             1473  ACATGC              
PflFI        1861  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2059  GACGCAGTC           
PpuMI        1733  GGGACCC              [GA]G^G[AT]CC[CT]
             1793  GGGTCCC             
Tth111I      1861  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2059  GACGCAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XhoII       
XmaCI       XmaI        
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