Restriction map for pF1KB3518
 (Linear) (Six-base) MAPSORT of: KIBB3518.seq  Check: 1742  from: 1  to: 2280



KIBB3518.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2077    2280 
   Size:       2077     203
 
AatII G_ACGT'C

Cuts at:      0    1178    2280 
   Size:       1178    1102
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     394     424    1659    2280 
   Size:        283     111      30    1235     621

  Fragments arranged by size: 

                1235     621     283     111      30
 
AfeI AGC'GCT

Cuts at:      0     766    2280 
   Size:        766    1514
 
AhdI GACnn_n'nnGTC

Cuts at:      0     582    2280 
   Size:        582    1698
 
AleI CACnn'nnGTG

Cuts at:      0    1804    2280 
   Size:       1804     476
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1575    2085    2280 
   Size:       1575     510     195

  Fragments arranged by size: 

                1575     510     195
 
AlwNI CAG_nnn'CTG

Cuts at:      0     543     676    1695    2280 
   Size:        543     133    1019     585

  Fragments arranged by size: 

                1019     585     543     133
 
ApaI G_GGCC'C

Cuts at:      0      76     127     167     178     657    1498    2280 
   Size:         76      51      40      11     479     841     782

  Fragments arranged by size: 

                 841     782     479      76      51      40      11
 
ApaLI G'TGCA_C

Cuts at:      0    1008    2035    2280 
   Size:       1008    1027     245

  Fragments arranged by size: 

                1027    1008     245
 
AvaI C'yCGr_G

Cuts at:      0     115     160    1913    2280 
   Size:        115      45    1753     367

  Fragments arranged by size: 

                1753     367     115      45
 
BamHI G'GATC_C

Cuts at:      0     200    1596    2280 
   Size:        200    1396     684

  Fragments arranged by size: 

                1396     684     200
 
BanI G'GyrC_C

Cuts at:      0     897    1717    2280 
   Size:        897     820     563

  Fragments arranged by size: 

                 897     820     563
 
BanII G_rGCy'C

Cuts at:      0      76     127     167     178     185     626     657     666 
   Size:         76      51      40      11       7     441      31       9

Cuts at:    666     686     970     993    1027    1488    1498    2280 
   Size:         20     284      23      34     461      10     782

  Fragments arranged by size: 

                 782     461     441     284      76      51      40      34
                  31      23      20      11      10       9       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     827     917     932    1170    1303    2265    2280 
   Size:        827      90      15     238     133     962      15

  Fragments arranged by size: 

                 962     827     238     133      90      15      15
 
BbvCI CC'TCA_GC

Cuts at:      0    1258    1588    2280 
   Size:       1258     330     692

  Fragments arranged by size: 

                1258     692     330
 
BglI GCCn_nnn'nGGC

Cuts at:      0     661    2280 
   Size:        661    1619
 
BglII A'GATC_T

Cuts at:      0    1230    2280 
   Size:       1230    1050
 
BlpI GC'TnA_GC

Cuts at:      0    1627    2280 
   Size:       1627     653
 
Bme1580I G_kGCm'C

Cuts at:      0      76     127     167     178     657     902    1012    1498 
   Size:         76      51      40      11     479     245     110     486

Cuts at:   1498    1896    2039    2280 
   Size:        398     143     241

  Fragments arranged by size: 

                 486     479     398     245     241     143     110      76
                  51      40      11
 
BmgBI CAC'GTC

Cuts at:      0    1301    1635    2280 
   Size:       1301     334     645

  Fragments arranged by size: 

                1301     645     334
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      95    1492    2280 
   Size:         95    1397     788

  Fragments arranged by size: 

                1397     788      95
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     640     977    1660    1927    2157    2280 
   Size:        511     129     337     683     267     230     123

  Fragments arranged by size: 

                 683     511     337     267     230     129     123
 
Bpu10I CC'TnA_GC

Cuts at:      0      63     187     284     969    1258    1588    2280 
   Size:         63     124      97     685     289     330     692

  Fragments arranged by size: 

                 692     685     330     289     124      97      63
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1390    2280 
   Size:       1390     890
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     105    2280 
   Size:        105    2175
 
BsaHI Gr'CG_yC

Cuts at:      0    1175    1655    2280 
   Size:       1175     480     625

  Fragments arranged by size: 

                1175     625     480
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22      33     736    1158    1888    2209    2280 
   Size:         22      11     703     422     730     321      71

  Fragments arranged by size: 

                 730     703     422     321      71      22      11
 
BseYI C'CCAG_C

Cuts at:      0     254     606     783    1133    1688    1738    2224    2280 
   Size:        254     352     177     350     555      50     486      56

  Fragments arranged by size: 

                 555     486     352     350     254     177      56      50
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     874    1749    2182    2280 
   Size:        190     684     875     433      98

  Fragments arranged by size: 

                 875     684     433     190      98
 
BsiEI CG_ry'CG

Cuts at:      0      20      29    2280 
   Size:         20       9    2251

  Fragments arranged by size: 

                2251      20       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     626    1012    1790    2039    2280 
   Size:        626     386     778     249     241

  Fragments arranged by size: 

                 778     626     386     249     241
 
BsmI GAATG_Cn'

Cuts at:      0    1064    1340    1452    2095    2280 
   Size:       1064     276     112     643     185

  Fragments arranged by size: 

                1064     643     276     185     112
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2137    2280 
   Size:       2137     143
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     127     167     178     185     626     657     666 
   Size:         76      51      40      11       7     441      31       9

Cuts at:    666     686     902     970     993    1012    1027    1488    1498 
   Size:         20     216      68      23      19      15     461      10

Cuts at:   1498    1790    1896    2039    2280 
   Size:        292     106     143     241

  Fragments arranged by size: 

                 461     441     292     241     216     143     106      76
                  68      51      40      31      23      20      19      15
                  11      10       9       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     121    1085    1427    2077    2280 
   Size:        121     964     342     650     203

  Fragments arranged by size: 

                 964     650     342     203     121
 
BsrBI CCG'CTC

Cuts at:      0      16      57     614    1853    2280 
   Size:         16      41     557    1239     427

  Fragments arranged by size: 

                1239     557     427      41      16
 
BsrDI GCAATG_nn'

Cuts at:      0    2237    2280 
   Size:       2237      43
 
BsrFI r'CCGG_y

Cuts at:      0    2031    2280 
   Size:       2031     249
 
BssHII G'CGCG_C

Cuts at:      0     229     267    2280 
   Size:        229      38    2013

  Fragments arranged by size: 

                2013     229      38
 
BssSI C'ACGA_G

Cuts at:      0     220    2191    2280 
   Size:        220    1971      89

  Fragments arranged by size: 

                1971     220      89
 
BstBI TT'CG_AA

Cuts at:      0     236    2280 
   Size:        236    2044
 
BstEII G'GTnAC_C

Cuts at:      0     483    2280 
   Size:        483    1797
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     894    1806    2280 
   Size:        894     912     474

  Fragments arranged by size: 

                 912     894     474
 
BstYI r'GATC_y

Cuts at:      0     200     791    1230    1596    2280 
   Size:        200     591     439     366     684

  Fragments arranged by size: 

                 684     591     439     366     200
 
BtgI C'CryG_G

Cuts at:      0      23     167     473    1647    2042    2078    2280 
   Size:         23     144     306    1174     395      36     202

  Fragments arranged by size: 

                1174     395     306     202     144      36      23
 
BtsI GCAGTG_nn'

Cuts at:      0     641    1896    2280 
   Size:        641    1255     384

  Fragments arranged by size: 

                1255     641     384
 
DraIII CAC_nnn'GTG

Cuts at:      0    1839    2280 
   Size:       1839     441
 
EaeI y'GGCC_r

Cuts at:      0      17    1141    1436    2264    2280 
   Size:         17    1124     295     828      16

  Fragments arranged by size: 

                1124     828     295      17      16
 
EagI C'GGCC_G

Cuts at:      0      17    2280 
   Size:         17    2263
 
EarI CTCTTCn'nnn_

Cuts at:      0     542    2280 
   Size:        542    1738
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      93     164     363    2280 
   Size:         93      71     199    1917

  Fragments arranged by size: 

                1917     199      93      71
 
EcoICRI GAG'CTC

Cuts at:      0     624    2280 
   Size:        624    1656
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     394     424     511     640     977    1659    1660 
   Size:        283     111      30      87     129     337     682       1

Cuts at:   1660    1927    2157    2280 
   Size:        267     230     123

  Fragments arranged by size: 

                 682     337     283     267     230     129     123     111
                  87      30       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     424     547    1423    2266    2280 
   Size:        424     123     876     843      14

  Fragments arranged by size: 

                 876     843     424     123      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73     653     654     673    1494    1665    1734    1794 
   Size:         73     580       1      19     821     171      69      60

Cuts at:   1794    2232    2280 
   Size:        438      48

  Fragments arranged by size: 

                 821     580     438     171      73      69      60      48
                  19       1
 
HaeII r_GCGC'y

Cuts at:      0     768    1456    1695    2255    2280 
   Size:        768     688     239     560      25

  Fragments arranged by size: 

                 768     688     560     239      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     568     600    2280 
   Size:        568      32    1680

  Fragments arranged by size: 

                1680     568      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     558    1552    1954    2280 
   Size:        558     994     402     326

  Fragments arranged by size: 

                 994     558     402     326
 
MscI TGG'CCA

Cuts at:      0    2266    2280 
   Size:       2266      14
 
MslI CAynn'nnrTG

Cuts at:      0    1804    2280 
   Size:       1804     476
 
MspA1I CmG'CkG

Cuts at:      0      25     254    1262    1925    2280 
   Size:         25     229    1008     663     355

  Fragments arranged by size: 

                1008     663     355     229      25
 
NcoI C'CATG_G

Cuts at:      0    2042    2280 
   Size:       2042     238
 
NotI GC'GGCC_GC

Cuts at:      0      17    2280 
   Size:         17    2263
 
NspI r_CATG'y

Cuts at:      0     582    1477    2280 
   Size:        582     895     803

  Fragments arranged by size: 

                 895     803     582
 
PasI CC'CwG_GG

Cuts at:      0     659    2280 
   Size:        659    1621
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1035    1638    1695    1839    2280 
   Size:       1035     603      57     144     441

  Fragments arranged by size: 

                1035     603     441     144      57
 
PfoI T'CCnGG_A

Cuts at:      0    1637    2009    2280 
   Size:       1637     372     271

  Fragments arranged by size: 

                1637     372     271
 
PpuMI rG'GwC_Cy

Cuts at:      0    1734    1794    2280 
   Size:       1734      60     486

  Fragments arranged by size: 

                1734     486      60
 
PspOMI G'GGCC_C

Cuts at:      0      72     123     163     174     653    1494    2280 
   Size:         72      51      40      11     479     841     786

  Fragments arranged by size: 

                 841     786     479      72      51      40      11
 
PstI C_TGCA'G

Cuts at:      0     681     806    1424    2280 
   Size:        681     125     618     856

  Fragments arranged by size: 

                 856     681     618     125
 
PvuII CAG'CTG

Cuts at:      0    1262    2280 
   Size:       1262    1018
 
SacI G_AGCT'C

Cuts at:      0     626    2280 
   Size:        626    1654
 
SacII CC_GC'GG

Cuts at:      0      26    2280 
   Size:         26    2254
 
SanDI GG'GwC_CC

Cuts at:      0    1734    1794    2280 
   Size:       1734      60     486

  Fragments arranged by size: 

                1734     486      60
 
SbfI CC_TGCA'GG

Cuts at:      0     681    2280 
   Size:        681    1599
 
SexAI A'CCwGG_T

Cuts at:      0     338    2280 
   Size:        338    1942
 
SfcI C'TryA_G

Cuts at:      0     677     802    1420    1539    2280 
   Size:        677     125     618     119     741

  Fragments arranged by size: 

                 741     677     618     125     119
 
SmaI CCC'GGG

Cuts at:      0     117     162    1915    2280 
   Size:        117      45    1753     365

  Fragments arranged by size: 

                1753     365     117      45
 
SmlI C'TyrA_G

Cuts at:      0    1405    2280 
   Size:       1405     875
 
StyI C'CwwG_G

Cuts at:      0     887    1059    2042    2267    2280 
   Size:        887     172     983     225      13

  Fragments arranged by size: 

                 983     887     225     172      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     471    2280 
   Size:        471    1809
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1645    1677    2280 
   Size:       1645      32     603

  Fragments arranged by size: 

                1645     603      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1864    2062    2280 
   Size:       1864     198     218

  Fragments arranged by size: 

                1864     218     198
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     471    2280 
   Size:        471    1809
 
XmaI C'CCGG_G

Cuts at:      0     115     160    1913    2280 
   Size:        115      45    1753     367

  Fragments arranged by size: 

                1753     367     115      45
 
ZraI GAC'GTC

Cuts at:      0    1176    2280 
   Size:       1176    1104
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI     BtgI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I     NcoI     NotI
     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII
     SacI    SacII    SanDI     SbfI    SexAI     SfcI     SmaI     SmlI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AloI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI
     BclI    BfrBI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiWI    BspEI    BspHI    BsrGI   BstAPI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SalI     SapI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XhoI     XmnI
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