Restriction map for pF1KB3487
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
AvrII       BamHI       BbvCI       BfrI        BglII       
BlnI        BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DraIII      EagI        EclXI       Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        1180  GACGTC               GACGT^C
AccI         1090  GTCTAC               GT^[AC][GT]AC
AcsI          181  GAATTT               [GA]^AATT[CT]
AhdI         1006  GACCTGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1263  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        34  AGCGCT               AGC^GCT
ApoI          181  GAATTT               [GA]^AATT[CT]
Asp700I      1647  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1006  GACCTGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1858  GAGCAC               G[AT]GC[AT]^C
BanII         384  GGGCTC               G[GA]GC[CT]^C
BbeI           41  GGCGCC               GGCGC^C
BbrPI        1270  CACGTG               CAC^GTG
BcgI          552  GCAGGCCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         162  GTATCC               GTATCC(6/5)
BclI          836  TGATCA               T^GATCA
BglI         1136  GCCTGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           30  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       30  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1270  CACGTG               [CT]AC^GT[GA]
BsiHKAI      1858  GAGCAC               G[AT]GC[AT]^C
BssHII       1769  GCGCGC               G^CGCGC
Bsu36I       1263  CCTGAGG              CC^T[ACGT]AGG
CelII          30  GCTGAGC              GC^T[ACGT]AGC
DrdI         1375  GACCTGGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1006  GACCTGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       34  AGCGCT               AGC^GCT
Eco81I       1263  CCTGAGG              CC^T[ACGT]AGG
EcoNI         237  CCTTTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          836  TGATCA               T^GATCA
HgaI         1012  GCGTC                GACGC(5/10)
HphI          839  TCACC                GGTGA(8/7)
KasI           41  GGCGCC               G^GCGCC
NarI           41  GGCGCC               GG^CGCC
NruI          998  TCGCGA               TCG^CGA
PmaCI        1270  CACGTG               CAC^GTG
PmlI         1270  CACGTG               CAC^GTG
SfoI           41  GGCGCC               GGC^GCC
SmlI          532  CTCAAG               C^T[CT][GA]AG
XbaI         1015  TCTAGA               T^CTAGA
XmnI         1647  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwNI        1258  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1705  CAGAACCTG           
AvaI           89  CCCGAG               C^[CT]CG[GA]G
             1354  CTCGGG              
BanI           41  GGCGCC               G^G[CT][GA]CC
             1342  GGTGCC              
BbsI         1647  GAAGAC               GAAGAC(2/6)
             1783  GAAGAC              
BpuAI        1647  GAAGAC               GAAGAC(2/6)
             1783  GAAGAC              
BseRI         343  GAGGAG               GAGGAG(10/8)
             1855  GAGGAG              
BsgI          795  CTGCAC               GTGCAG(16/14)
             1102  CTGCAC              
BsiEI        1033  CGGTCG               CG[GA][CT]^CG
             1656  CGACCG              
BsmI          947  GCATTC               GAATGC(1/-1)
             1235  GCATTC              
BsoBI          89  CCCGAG               C^[CT]CG[GA]G
             1354  CTCGGG              
BspHI         177  TCATGA               T^CATGA
             1478  TCATGA              
BspMI        1094  ACCTGC               ACCTGC(4/8)
             1257  GCAGGT              
BstYI         666  GGATCT               [GA]^GATC[CT]
             1049  AGATCC              
DsaI          446  CCGTGG               C^C[GA][CT]GG
              693  CCGTGG              
EarI         1061  GAAGAG               CTCTTC(1/4)
             1414  GAAGAG              
EcoT14I        56  CCAAGG               C^C[AT][AT]GG
              662  CCTTGG              
Ksp632I      1061  GAAGAG               CTCTTC(1/4)
             1414  GAAGAG              
MflI          666  GGATCT               [GA]^GATC[CT]
             1049  AGATCC              
MslI          828  CACGGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1270  CACGTGAATG          
PflMI          56  CCAAGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1456  CCAAACGCTGG         
RcaI          177  TCATGA               T^CATGA
             1478  TCATGA              
StuI          849  AGGCCT               AGG^CCT
             1426  AGGCCT              
StyI           56  CCAAGG               C^C[AT][AT]GG
              662  CCTTGG              
Van91I         56  CCAAGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1456  CCAAACGCTGG         
XcmI          638  CCAGCTATTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              807  CCACACCAGCACTGG     
XhoII         666  GGATCT               [GA]^GATC[CT]
             1049  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaII       BalI        BbvI        BcnI        
BfaI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        SphI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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