Restriction map for pF1KB3472
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbvCI       BcgI        BciVI       
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BsaHI       BsaI        BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoT14I     
EcoT22I     FseI        FspI        Hin1I       HpaI        
KasI        KpnI        KspI        MluI        MroI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         1870  GTAGAC               GT^[AC][GT]AC
AflII         997  CTTAAG               C^TTAAG
Aor51HI      1877  AGCGCT               AGC^GCT
Asp700I       705  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1926  GTGCTC               G[AT]GC[AT]^C
AvaI         2259  CCCGAG               C^[CT]CG[GA]G
BalI         2120  TGGCCA               TGG^CCA
BanI         1858  GGTGCC               G^G[CT][GA]CC
BanII         298  GAGCCC               G[GA]GC[CT]^C
BbrPI        1493  CACGTG               CAC^GTG
BfrI          997  CTTAAG               C^TTAAG
BglII         984  AGATCT               A^GATCT
BsaAI        1493  CACGTG               [CT]AC^GT[GA]
BsaBI         924  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         474  ACCGGA               [AT]^CCGG[AT]
BsgI         1490  CTGCAC               GTGCAG(16/14)
BsiHKAI      1926  GTGCTC               G[AT]GC[AT]^C
BsoBI        2259  CCCGAG               C^[CT]CG[GA]G
BspDI        1568  ATCGAT               AT^CGAT
Cac8I        2105  GCTGGC               GC[ACGT]^[ACGT]GC
ClaI         1568  ATCGAT               AT^CGAT
DsaI         2149  CCACGG               C^C[GA][CT]GG
EaeI         2120  TGGCCA               [CT]^GGCC[GA]
Eco47III     1877  AGCGCT               AGC^GCT
EcoRV         928  GATATC               GAT^ATC
HincII        199  GTTGAC               GT[CT]^[GA]AC
HindII        199  GTTGAC               GT[CT]^[GA]AC
HindIII      1718  AAGCTT               A^AGCTT
MamI          924  GATTGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1931  CAATTG               C^AATTG
MluNI        2120  TGGCCA               TGG^CCA
MscI         2120  TGGCCA               TGG^CCA
MslI           42  CACCGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1931  CAATTG               C^AATTG
NdeI         1178  CATATG               CA^TATG
NspI         1002  GCATGT               [GA]CATG^[CT]
PflMI        1026  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1493  CACGTG               CAC^GTG
PmlI         1493  CACGTG               CAC^GTG
SexAI         945  ACCAGGT              A^CC[AT]GGT
SmlI          997  CTTAAG               C^T[CT][GA]AG
SspI         1945  AATATT               AAT^ATT
Van91I       1026  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          705  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI          207  GAATTC               [GA]^AATT[CT]
             1368  GAATTC              
AlwI         1571  GATCC                GGATC(4/5)
             2300  GATCC               
AocI          781  CCTCAGG              CC^T[ACGT]AGG
             1510  CCTGAGG             
ApoI          207  GAATTC               [GA]^AATT[CT]
             1368  GAATTC              
BbsI          705  GAAGAC               GAAGAC(2/6)
             1096  GAAGAC              
BclI         1050  TGATCA               T^GATCA
             1886  TGATCA              
BcnI            4  CCGGG                CC^[GC]GG
             1574  CCGGG               
BfaI          428  CTAG                 C^TAG
              982  CTAG                
BpuAI         705  GAAGAC               GAAGAC(2/6)
             1096  GAAGAC              
BsmAI          61  GAGAC                GTCTC(1/5)
             1406  GAGAC               
BsrDI        1112  CATTGC               GCAATG(2/0)
               46  GCAATG              
BstYI         984  AGATCT               [GA]^GATC[CT]
             2299  AGATCC              
Bsu36I        781  CCTCAGG              CC^T[ACGT]AGG
             1510  CCTGAGG             
CfoI          557  GCGC                 GCG^C
             1878  GCGC                
Eco81I        781  CCTCAGG              CC^T[ACGT]AGG
             1510  CCTGAGG             
EcoRI         207  GAATTC               G^AATTC
             1368  GAATTC              
FbaI         1050  TGATCA               T^GATCA
             1886  TGATCA              
HaeII         556  GGCGCT               [GA]GCGC^[CT]
             1877  AGCGCT              
HhaI          557  GCGC                 GCG^C
             1878  GCGC                
HinP1I        557  GCGC                 G^CGC
             1878  GCGC                
HphI          989  TCACC                GGTGA(8/7)
             1433  TCACC               
MaeI          428  CTAG                 C^TAG
              982  CTAG                
MaeII        1494  ACGT                 A^CGT
             1864  ACGT                
MflI          984  AGATCT               [GA]^GATC[CT]
             2299  AGATCC              
NciI            4  CCGGG                CC^[GC]GG
             1574  CCGGG               
PvuII        1790  CAGCTG               CAG^CTG
             1978  CAGCTG              
TaqI         1569  TCGA                 T^CGA
             2193  TCGA                
TthHB8I      1569  TCGA                 T^CGA
             2193  TCGA                
XcmI          963  CCACATACAGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1736  CCATCAGGCTTTTGG     
XhoII         984  AGATCT               [GA]^GATC[CT]
             2299  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BbvI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EarI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HinfI       HpaII       
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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