Restriction map for pF1KE0298
 (Linear) (Six-base) MAPSORT of: KIEE0298.seq  Check: 4941  from: 1  to: 8154



KIEE0298.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    5631    7852    7860    8154 
   Size:       5631    2221       8     294

  Fragments arranged by size: 

                5631    2221     294       8
 
AccI GT'mk_AC

Cuts at:      0    3325    4600    5196    6875    8154 
   Size:       3325    1275     596    1679    1279

  Fragments arranged by size: 

                3325    1679    1279    1275     596
 
Acc65I G'GTAC_C

Cuts at:      0    7218    8154 
   Size:       7218     936
 
AclI AA'CG_TT

Cuts at:      0    4505    8154 
   Size:       4505    3649
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     380     395    1771    2699    3112    3157    3185 
   Size:        196     184      15    1376     928     413      45      28

Cuts at:   3185    3601    4688    5204    5615    5870    6649    7240    7414 
   Size:        416    1087     516     411     255     779     591     174

Cuts at:   7414    8014    8027    8088    8154 
   Size:        600      13      61      66

  Fragments arranged by size: 

                1376    1087     928     779     600     591     516     416
                 413     411     255     196     184     174      66      61
                  45      28      15      13
 
AflII C'TTAA_G

Cuts at:      0    1716    5841    6066    8154 
   Size:       1716    4125     225    2088

  Fragments arranged by size: 

                4125    2088    1716     225
 
AflIII A'CryG_T

Cuts at:      0    1820    3651    8154 
   Size:       1820    1831    4503

  Fragments arranged by size: 

                4503    1831    1820
 
AleI CACnn'nnGTG

Cuts at:      0    7246    7339    8154 
   Size:       7246      93     815

  Fragments arranged by size: 

                7246     815      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1421    6857    7405    8154 
   Size:       1421    5436     548     749

  Fragments arranged by size: 

                5436    1421     749     548
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1713    1745    2394    2426    7553    7585    8154 
   Size:       1713      32     649      32    5127      32     569

  Fragments arranged by size: 

                5127    1713     649     569      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1408    2551    2754    3492    5790    7572    8154 
   Size:       1408    1143     203     738    2298    1782     582

  Fragments arranged by size: 

                2298    1782    1408    1143     738     582     203
 
ApaI G_GGCC'C

Cuts at:      0    3298    8154 
   Size:       3298    4856
 
ApaLI G'TGCA_C

Cuts at:      0    1546    1766    8098    8154 
   Size:       1546     220    6332      56

  Fragments arranged by size: 

                6332    1546     220      56
 
ApoI r'AATT_y

Cuts at:      0      17     463    1860    2916    2935    4626    5049    5107 
   Size:         17     446    1397    1056      19    1691     423      58

Cuts at:   5107    5145    5533    5569    6109    6159    6932    8154 
   Size:         38     388      36     540      50     773    1222

  Fragments arranged by size: 

                1691    1397    1222    1056     773     540     446     423
                 388      58      50      38      36      19      17
 
AseI AT'TA_AT

Cuts at:      0    4112    4813    8154 
   Size:       4112     701    3341

  Fragments arranged by size: 

                4112    3341     701
 
AvaI C'yCGr_G

Cuts at:      0    3560    4232    8154 
   Size:       3560     672    3922

  Fragments arranged by size: 

                3922    3560     672
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    7425    7458    8154 
   Size:       7425      33     696

  Fragments arranged by size: 

                7425     696      33
 
BamHI G'GATC_C

Cuts at:      0    1743    3312    7208    8154 
   Size:       1743    1569    3896     946

  Fragments arranged by size: 

                3896    1743    1569     946
 
BanI G'GyrC_C

Cuts at:      0    1495    1525    4887    7218    8154 
   Size:       1495      30    3362    2331     936

  Fragments arranged by size: 

                3362    2331    1495     936      30
 
BanII G_rGCy'C

Cuts at:      0     643    3298    4859    5420    6982    7704    8154 
   Size:        643    2655    1561     561    1562     722     450

  Fragments arranged by size: 

                2655    1562    1561     722     643     561     450
 
BbeI G_GCGC'C

Cuts at:      0    1499    1529    8154 
   Size:       1499      30    6625

  Fragments arranged by size: 

                6625    1499      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     761    1475    2714    4637    6578    6814    8154 
   Size:        761     714    1239    1923    1941     236    1340

  Fragments arranged by size: 

                1941    1923    1340    1239     761     714     236
 
BbvCI CC'TCA_GC

Cuts at:      0    4400    8154 
   Size:       4400    3754
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     138     172    4143    4177    8154 
   Size:        138      34    3971      34    3977

  Fragments arranged by size: 

                3977    3971     138      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    6465    8154 
   Size:       6465    1689
 
BclI T'GATC_A

Cuts at:      0    3756    8154 
   Size:       3756    4398
 
BfrBI ATG'CAT

Cuts at:      0     536    8154 
   Size:        536    7618
 
BglI GCCn_nnn'nGGC

Cuts at:      0    7612    8154 
   Size:       7612     542
 
BglII A'GATC_T

Cuts at:      0    1024    1648    4986    7634    8154 
   Size:       1024     624    3338    2648     520

  Fragments arranged by size: 

                3338    2648    1024     624     520
 
BlpI GC'TnA_GC

Cuts at:      0    7013    8154 
   Size:       7013    1141
 
Bme1580I G_kGCm'C

Cuts at:      0    1550    1770    2663    3008    3298    7253    8102    8154 
   Size:       1550     220     893     345     290    3955     849      52

  Fragments arranged by size: 

                3955    1550     893     849     345     290     220      52
            
 
BmgBI CAC'GTC

Cuts at:      0    5328    7979    8112    8154 
   Size:       5328    2651     133      42

  Fragments arranged by size: 

                5328    2651     133      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1393    3197    3469    4256    4364    8154 
   Size:       1393    1804     272     787     108    3790

  Fragments arranged by size: 

                3790    1804    1393     787     272     108
 
BmtI G_CTAG'C

Cuts at:      0    6635    8154 
   Size:       6635    1519
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     224    8154 
   Size:        224    7930
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1307    1824    3612    3891    4476    5675    7094    7385 
   Size:       1307     517    1788     279     585    1199    1419     291

Cuts at:   7385    7615    7688    8030    8154 
   Size:        230      73     342     124

  Fragments arranged by size: 

                1788    1419    1307    1199     585     517     342     291
                 279     230     124      73
 
Bpu10I CC'TnA_GC

Cuts at:      0    2990    3299    3424    3452    4400    4858    6778    8154 
   Size:       2990     309     125      28     948     458    1920    1376

  Fragments arranged by size: 

                2990    1920    1376     948     458     309     125      28
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     477    2251    3654    7058    8154 
   Size:        477    1774    1403    3404    1096

  Fragments arranged by size: 

                3404    1774    1403    1096     477
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3521    3575    3728    4143    5325    7633    8154 
   Size:       3521      54     153     415    1182    2308     521

  Fragments arranged by size: 

                3521    2308    1182     521     415     153      54
 
BsaAI yAC'GTr

Cuts at:      0    1914    7929    8154 
   Size:       1914    6015     225

  Fragments arranged by size: 

                6015    1914     225
 
BsaBI GATnn'nnATC

Cuts at:      0     438    3532    4322    8154 
   Size:        438    3094     790    3832

  Fragments arranged by size: 

                3832    3094     790     438
 
BsaHI Gr'CG_yC

Cuts at:      0    1496    1526    3073    6443    8154 
   Size:       1496      30    1547    3370    1711

  Fragments arranged by size: 

                3370    1711    1547    1496      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2394    2424    3403    3433    3903    3933    5945    5975 
   Size:       2394      30     979      30     470      30    2012      30

Cuts at:   5975    6801    6831    7779    7809    8028    8058    8154 
   Size:        826      30     948      30     219      30      96

  Fragments arranged by size: 

                2394    2012     979     948     826     470     219      96
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1028    1501    2248    2558    2561    2820    3623    7787 
   Size:       1028     473     747     310       3     259     803    4164

Cuts at:   7787    8154 
   Size:        367

  Fragments arranged by size: 

                4164    1028     803     747     473     367     310     259
                   3
 
BseYI C'CCAG_C

Cuts at:      0    1931    2089    3046    3788    5247    5783    7163    8154 
   Size:       1931     158     957     742    1459     536    1380     991

  Fragments arranged by size: 

                1931    1459    1380     991     957     742     536     158
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671    4900    6336    7291    8154 
   Size:        671    4229    1436     955     863

  Fragments arranged by size: 

                4229    1436     955     863     671
 
BsiHKAI G_wGCw'C

Cuts at:      0     625    1550    1770    2996    4579    4859    6784    7019 
   Size:        625     925     220    1226    1583     280    1925     235

Cuts at:   7019    7704    8102    8154 
   Size:        685     398      52

  Fragments arranged by size: 

                1925    1583    1226     925     685     625     398     280
                 235     220      52
 
BsmI GAATG_Cn'

Cuts at:      0     538     583     960    3189    4383    4734    5088    7158 
   Size:        538      45     377    2229    1194     351     354    2070

Cuts at:   7158    7557    8154 
   Size:        399     597

  Fragments arranged by size: 

                2229    2070    1194     597     538     399     377     354
                 351      45
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    6450    8154 
   Size:       6450    1704
 
Bsp1286I G_dGCh'C

Cuts at:      0     625     643    1550    1770    2663    2996    3008    3298 
   Size:        625      18     907     220     893     333      12     290

Cuts at:   3298    4579    4859    5420    6784    6982    7019    7253    7704 
   Size:       1281     280     561    1364     198      37     234     451

Cuts at:   7704    8102    8154 
   Size:        398      52

  Fragments arranged by size: 

                1364    1281     907     893     625     561     451     398
                 333     290     280     234     220     198      52      37
                  18      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2559    5631    7230    7557    7852    7860    8154 
   Size:       2559    3072    1599     327     295       8     294

  Fragments arranged by size: 

                3072    2559    1599     327     295     294       8
 
BsrBI CCG'CTC

Cuts at:      0    1277    2238    8154 
   Size:       1277     961    5916

  Fragments arranged by size: 

                5916    1277     961
 
BsrDI GCAATG_nn'

Cuts at:      0     611     833    2182    5264    7648    8154 
   Size:        611     222    1349    3082    2384     506

  Fragments arranged by size: 

                3082    2384    1349     611     506     222
 
BsrGI T'GTAC_A

Cuts at:      0    4335    5662    8154 
   Size:       4335    1327    2492

  Fragments arranged by size: 

                4335    2492    1327
 
BssSI C'ACGA_G

Cuts at:      0    1429    8154 
   Size:       1429    6725
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1545    2551    4740    4842    4887    7733    8154 
   Size:       1545    1006    2189     102      45    2846     421

  Fragments arranged by size: 

                2846    2189    1545    1006     421     102      45
 
BstBI TT'CG_AA

Cuts at:      0    5613    8154 
   Size:       5613    2541
 
BstEII G'GTnAC_C

Cuts at:      0    2084    7805    7864    8154 
   Size:       2084    5721      59     290

  Fragments arranged by size: 

                5721    2084     290      59
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2785    8154 
   Size:       2785    5369
 
BstYI r'GATC_y

Cuts at:      0     439    1024    1648    1743    3312    4467    4761    4986 
   Size:        439     585     624      95    1569    1155     294     225

Cuts at:   4986    7208    7634    8154 
   Size:       2222     426     520

  Fragments arranged by size: 

                2222    1569    1155     624     585     520     439     426
                 294     225      95
 
Bsu36I CC'TnA_GG

Cuts at:      0    7610    7799    8025    8154 
   Size:       7610     189     226     129

  Fragments arranged by size: 

                7610     226     189     129
 
BtgI C'CryG_G

Cuts at:      0    1529    1739    2305    3308    5270    8080    8154 
   Size:       1529     210     566    1003    1962    2810      74

  Fragments arranged by size: 

                2810    1962    1529    1003     566     210      74
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    8135    8154 
   Size:       8135      19
 
BtsI GCAGTG_nn'

Cuts at:      0     578     992    4388    6348    7389    8154 
   Size:        578     414    3396    1960    1041     765

  Fragments arranged by size: 

                3396    1960    1041     765     578     414
 
ClaI AT'CG_AT

Cuts at:      0    4007    8154 
   Size:       4007    4147
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    5918    5931    5953    5966    6087    6122    8154 
   Size:       5918      13      22      13     121      35    2032

  Fragments arranged by size: 

                5918    2032     121      35      22      13      13
 
DraI TTT'AAA

Cuts at:      0    1265    2364    2387    4304    8154 
   Size:       1265    1099      23    1917    3850

  Fragments arranged by size: 

                3850    1917    1265    1099      23
 
DraIII CAC_nnn'GTG

Cuts at:      0     649    8154 
   Size:        649    7505
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    6037    6691    8154 
   Size:       6037     654    1463

  Fragments arranged by size: 

                6037    1463     654
 
EaeI y'GGCC_r

Cuts at:      0    2216    7407    8154 
   Size:       2216    5191     747

  Fragments arranged by size: 

                5191    2216     747
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     233     546    2718    4370    4725    6218    6246 
   Size:        215      18     313    2172    1652     355    1493      28

Cuts at:   6246    8154 
   Size:       1908

  Fragments arranged by size: 

                2172    1908    1652    1493     355     313     215      28
                  18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    6328    8154 
   Size:       6328    1826
 
EcoICRI GAG'CTC

Cuts at:      0    4857    7702    8154 
   Size:       4857    2845     452

  Fragments arranged by size: 

                4857    2845     452
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     380     395    1307    1771    1824    2699    3112 
   Size:        196     184      15     912     464      53     875     413

Cuts at:   3112    3157    3185    3601    3612    3891    4476    4688    5204 
   Size:         45      28     416      11     279     585     212     516

Cuts at:   5204    5615    5675    5870    6649    7094    7240    7385    7414 
   Size:        411      60     195     779     445     146     145      29

Cuts at:   7414    7615    7688    8014    8027    8030    8088    8154 
   Size:        201      73     326      13       3      58      66

  Fragments arranged by size: 

                 912     875     779     585     516     464     445     416
                 413     411     326     279     212     201     196     195
                 184     146     145      73      66      60      58      53
                  45      29      28      15      13      11       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1782    2644    3050    3294    3295    4396    4862    6669 
   Size:       1782     862     406     244       1    1101     466    1807

Cuts at:   6669    6887    8154 
   Size:        218    1267

  Fragments arranged by size: 

                1807    1782    1267    1101     862     466     406     244
                 218       1
 
EcoRI G'AATT_C

Cuts at:      0    1860    6109    8154 
   Size:       1860    4249    2045

  Fragments arranged by size: 

                4249    2045    1860
 
EcoRV GAT'ATC

Cuts at:      0    2243    4533    8154 
   Size:       2243    2290    3621

  Fragments arranged by size: 

                3621    2290    2243
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     417    2488    3572    5971    6048    8154 
   Size:        417    2071    1084    2399      77    2106

  Fragments arranged by size: 

                2399    2106    2071    1084     417      77
 
HaeII r_GCGC'y

Cuts at:      0     452    1499    1529    2618    5915    6757    8154 
   Size:        452    1047      30    1089    3297     842    1397

  Fragments arranged by size: 

                3297    1397    1089    1047     842     452      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2227    2259    2658    2690    3297    3329    4810    4842 
   Size:       2227      32     399      32     607      32    1481      32

Cuts at:   4842    4971    5003    5500    5532    6801    6833    7779    7811 
   Size:        129      32     497      32    1269      32     946      32

Cuts at:   7811    8028    8060    8154 
   Size:        217      32      94

  Fragments arranged by size: 

                2227    1481    1269     946     607     497     399     217
                 129      94      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2150    3326    5197    5740    8154 
   Size:       2150    1176    1871     543    2414

  Fragments arranged by size: 

                2414    2150    1871    1176     543
 
HindIII A'AGCT_T

Cuts at:      0    1593    2248    2267    8154 
   Size:       1593     655      19    5887

  Fragments arranged by size: 

                5887    1593     655      19
 
KasI G'GCGC_C

Cuts at:      0    1495    1525    8154 
   Size:       1495      30    6629

  Fragments arranged by size: 

                6629    1495      30
 
KpnI G_GTAC'C

Cuts at:      0    7222    8154 
   Size:       7222     932
 
MfeI C'AATT_G

Cuts at:      0    4681    8154 
   Size:       4681    3473
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     905    1826    4749    5417    7539    8154 
   Size:        905     921    2923     668    2122     615

  Fragments arranged by size: 

                2923    2122     921     905     668     615
 
MscI TGG'CCA

Cuts at:      0    2218    8154 
   Size:       2218    5936
 
MslI CAynn'nnrTG

Cuts at:      0     857    2800    5081    5269    6332    6766    7246    7339 
   Size:        857    1943    2281     188    1063     434     480      93

Cuts at:   7339    8154 
   Size:        815

  Fragments arranged by size: 

                2281    1943    1063     857     815     480     434     188
                  93
 
MspA1I CmG'CkG

Cuts at:      0    2307    4045    5506    5790    7012    7036    7821    8082 
   Size:       2307    1738    1461     284    1222      24     785     261

Cuts at:   8082    8154 
   Size:         72

  Fragments arranged by size: 

                2307    1738    1461    1222     785     284     261      72
                  24
 
NarI GG'CG_CC

Cuts at:      0    1496    1526    8154 
   Size:       1496      30    6628

  Fragments arranged by size: 

                6628    1496      30
 
NcoI C'CATG_G

Cuts at:      0    1529    1739    3308    5270    8154 
   Size:       1529     210    1569    1962    2884

  Fragments arranged by size: 

                2884    1962    1569    1529     210
 
NdeI CA'TA_TG

Cuts at:      0     794    8154 
   Size:        794    7360
 
NheI G'CTAG_C

Cuts at:      0    6631    8154 
   Size:       6631    1523
 
NsiI A_TGCA'T

Cuts at:      0     538    8154 
   Size:        538    7616
 
NspI r_CATG'y

Cuts at:      0    1244    1824    3655    4255    6306    7736    8154 
   Size:       1244     580    1831     600    2051    1430     418

  Fragments arranged by size: 

                2051    1831    1430    1244     600     580     418
 
PasI CC'CwG_GG

Cuts at:      0    1827    6749    8154 
   Size:       1827    4922    1405

  Fragments arranged by size: 

                4922    1827    1405
 
PciI A'CATG_T

Cuts at:      0    1820    3651    8154 
   Size:       1820    1831    4503

  Fragments arranged by size: 

                4503    1831    1820
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1053    1408    5790    8154 
   Size:       1053     355    4382    2364

  Fragments arranged by size: 

                4382    2364    1053     355
 
PfoI T'CCnGG_A

Cuts at:      0    3485    3907    4068    8154 
   Size:       3485     422     161    4086

  Fragments arranged by size: 

                4086    3485     422     161
 
PmlI CAC'GTG

Cuts at:      0    7929    8154 
   Size:       7929     225
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2394    2426    8154 
   Size:       2394      32    5728

  Fragments arranged by size: 

                5728    2394      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1782    2644    3050    6669    6887    8154 
   Size:       1782     862     406    3619     218    1267

  Fragments arranged by size: 

                3619    1782    1267     862     406     218
 
PsiI TTA'TAA

Cuts at:      0     977    7187    8154 
   Size:        977    6210     967

  Fragments arranged by size: 

                6210     977     967
 
PspOMI G'GGCC_C

Cuts at:      0    3294    8154 
   Size:       3294    4860
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    7299    7331    8154 
   Size:       7299      32     823

  Fragments arranged by size: 

                7299     823      32
 
PstI C_TGCA'G

Cuts at:      0     413    1541    1656    2133    3020    3380    4475    4922 
   Size:        413    1128     115     477     887     360    1095     447

Cuts at:   4922    7011    7568    7863    8154 
   Size:       2089     557     295     291

  Fragments arranged by size: 

                2089    1128    1095     887     557     477     447     413
                 360     295     291     115
 
PvuII CAG'CTG

Cuts at:      0    4045    5506    5790    7012    8154 
   Size:       4045    1461     284    1222    1142

  Fragments arranged by size: 

                4045    1461    1222    1142     284
 
SacI G_AGCT'C

Cuts at:      0    4859    7704    8154 
   Size:       4859    2845     450

  Fragments arranged by size: 

                4859    2845     450
 
SacII CC_GC'GG

Cuts at:      0    2308    8083    8154 
   Size:       2308    5775      71

  Fragments arranged by size: 

                5775    2308      71
 
SalI G'TCGA_C

Cuts at:      0    3324    5195    8154 
   Size:       3324    1871    2959

  Fragments arranged by size: 

                3324    2959    1871
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4370    4725    8154 
   Size:       4370     355    3429

  Fragments arranged by size: 

                4370    3429     355
 
SbfI CC_TGCA'GG

Cuts at:      0    7863    8154 
   Size:       7863     291
 
ScaI AGT'ACT

Cuts at:      0    6681    8154 
   Size:       6681    1473
 
SexAI A'CCwGG_T

Cuts at:      0    2193    7775    8154 
   Size:       2193    5582     379

  Fragments arranged by size: 

                5582    2193     379
 
SfcI C'TryA_G

Cuts at:      0     409     998    1537    1639    1652    2129    3016    3036 
   Size:        409     589     539     102      13     477     887      20

Cuts at:   3036    3376    4471    4918    5411    6872    7007    7099    7564 
   Size:        340    1095     447     493    1461     135      92     465

Cuts at:   7564    7760    7811    7859    8154 
   Size:        196      51      48     295

  Fragments arranged by size: 

                1461    1095     887     589     539     493     477     465
                 447     409     340     295     196     135     102      92
                  51      48      20      13
 
SfoI GGC'GCC

Cuts at:      0    1497    1527    8154 
   Size:       1497      30    6627

  Fragments arranged by size: 

                6627    1497      30
 
SmlI C'TyrA_G

Cuts at:      0     492    1716    2230    3669    4232    5841    6066    7073 
   Size:        492    1224     514    1439     563    1609     225    1007

Cuts at:   7073    8154 
   Size:       1081

  Fragments arranged by size: 

                1609    1439    1224    1081    1007     563     514     492
                 225
 
SpeI A'CTAG_T

Cuts at:      0    4408    4974    8154 
   Size:       4408     566    3180

  Fragments arranged by size: 

                4408    3180     566
 
SphI G_CATG'C

Cuts at:      0    7736    8154 
   Size:       7736     418
 
SspI AAT'ATT

Cuts at:      0    6197    7491    8154 
   Size:       6197    1294     663

  Fragments arranged by size: 

                6197    1294     663
 
StuI AGG'CCT

Cuts at:      0    1004    3170    7427    7499    8154 
   Size:       1004    2166    4257      72     655

  Fragments arranged by size: 

                4257    2166    1004     655      72
 
StyI C'CwwG_G

Cuts at:      0    1529    1739    2769    3308    4866    5270    6410    8154 
   Size:       1529     210    1030     539    1558     404    1140    1744

  Fragments arranged by size: 

                1744    1558    1529    1140    1030     539     404     210
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3849    4355    5797    7804    8154 
   Size:       3849     506    1442    2007     350

  Fragments arranged by size: 

                3849    2007    1442     506     350
 
TatI w'GTAC_w

Cuts at:      0    4335    4411    5407    5662    6679    8154 
   Size:       4335      76     996     255    1017    1475

  Fragments arranged by size: 

                4335    1475    1017     996     255      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1345    1377    3403    3435    4862    4894    5945    5977 
   Size:       1345      32    2026      32    1427      32    1051      32

Cuts at:   5977    6391    6423    8154 
   Size:        414      32    1731

  Fragments arranged by size: 

                2026    1731    1427    1345    1051     414      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    7791    8154 
   Size:       7791     363
 
XbaI T'CTAG_A

Cuts at:      0    6652    8154 
   Size:       6652    1502
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    7512    8154 
   Size:       7512     642
 
XhoI C'TCGA_G

Cuts at:      0    4232    8154 
   Size:       4232    3922
 
XmnI GAAnn'nnTTC

Cuts at:      0    2409    2517    3125    3170    6932    8154 
   Size:       2409     108     608      45    3762    1222

  Fragments arranged by size: 

                3762    2409    1222     608     108      45
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI     PsiI   PspOMI
     PsrI     PstI    PvuII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SmlI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AgeI     AhdI     AscI    AsiSI    AvrII    BsaWI
    BsiEI    BsiWI    BspEI    BspHI    BsrFI   BssHII  BstZ17I     EagI
    EcoNI     FseI     FspI    FspAI     HpaI     MluI     NaeI   NgoMIV
     NotI     NruI     PacI     PmeI    PshAI    PspXI     PvuI    RsrII
    SanDI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SwaI
     XmaI     ZraI
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