Restriction map for pF1KA1887
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      ApaLI       
AscI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BbeI        BciVI       BclI        BglII       
BlnI        BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco52I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NdeI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI         2435  GTCTAC               GT^[AC][GT]AC
AcsI         2028  AAATTC               [GA]^AATT[CT]
AfaI          339  GTAC                 GT^AC
AflII        2815  CTTAAG               C^TTAAG
AflIII       2683  ACACGT               A^C[GA][CT]GT
AocI         1337  CCTCAGG              CC^T[ACGT]AGG
Aor51HI        77  AGCGCT               AGC^GCT
ApoI         2028  AAATTC               [GA]^AATT[CT]
AseI         2602  ATTAAT               AT^TAAT
AsnI         2602  ATTAAT               AT^TAAT
BbrPI        2684  CACGTG               CAC^GTG
BcgI         2445  CGAGAGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2815  CTTAAG               C^TTAAG
BlpI         2770  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2770  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2684  CACGTG               [CT]AC^GT[GA]
Bsu36I       1337  CCTCAGG              CC^T[ACGT]AGG
CelII        2770  GCTGAGC              GC^T[ACGT]AGC
DraI         2025  TTTAAA               TTT^AAA
Eco47III       77  AGCGCT               AGC^GCT
Eco81I       1337  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2358  CCTCTCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII          77  AGCGCT               [GA]GCGC^[CT]
HgaI         1089  GCGTC                GACGC(5/10)
HindIII      1284  AAGCTT               A^AGCTT
MaeII        2685  ACGT                 A^CGT
NaeI         2645  GCCGGC               GCC^GGC
NcoI         2511  CCATGG               C^CATGG
NgoMIV       2645  GCCGGC               G^CCGGC
NheI          958  GCTAGC               G^CTAGC
PmaCI        2684  CACGTG               CAC^GTG
PmlI         2684  CACGTG               CAC^GTG
PshBI        2602  ATTAAT               AT^TAAT
RsaI          339  GTAC                 GT^AC
SacI         2345  GAGCTC               GAGCT^C
SexAI        1746  ACCTGGT              A^CC[AT]GGT
SgrAI         307  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         2815  CTTAAG               C^T[CT][GA]AG
XcmI         1671  CCACAGTTTATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AspHI         100  GTGCTC               G[AT]GC[AT]^C
             2345  GAGCTC              
AspI          571  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1745  GACCTGGTC           
BalI           50  TGGCCA               TGG^CCA
             2310  TGGCCA              
BamHI        2055  GGATCC               G^GATCC
             2781  GGATCC              
BglI         1773  GCCTGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2475  GCCTGCCAGGC         
BsiHKAI       100  GTGCTC               G[AT]GC[AT]^C
             2345  GAGCTC              
BsrFI         308  ACCGGC               [GA]^CCGG[CT]
             2645  GCCGGC              
BstEII        243  GGTGACC              G^GT[ACGT]ACC
             2236  GGTGACC             
BstPI         243  GGTGACC              G^GT[ACGT]ACC
             2236  GGTGACC             
BstXI        1272  CCACTCTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2225  CCAGCCTGCTGG        
CfoI           78  GCGC                 GCG^C
             2035  GCGC                
Cfr10I        308  ACCGGC               [GA]^CCGG[CT]
             2645  GCCGGC              
DrdI         1154  GACGGAGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2524  GACCCCCCAGTC        
EcoO65I       243  GGTGACC              G^GT[ACGT]ACC
             2236  GGTGACC             
HhaI           78  GCGC                 GCG^C
             2035  GCGC                
HinP1I         78  GCGC                 G^CGC
             2035  GCGC                
MluNI          50  TGGCCA               TGG^CCA
             2310  TGGCCA              
MscI           50  TGGCCA               TGG^CCA
             2310  TGGCCA              
MslI          784  CACTGTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1273  CACTCTGATG          
PflFI         571  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1745  GACCTGGTC           
StuI          518  AGGCCT               AGG^CCT
             2482  AGGCCT              
Tsp509I      1221  AATT                 ^AATT
             2029  AATT                
Tth111I       571  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1745  GACCTGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       ApaI        
AvaI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XhoII       
XmaCI       XmaI        
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