Restriction map for pF1KA1849
 (Linear) (Six-base) MAPSORT of: KIAA1849.seq  Check: 9049  from: 1  to: 3219



KIAA1849.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1863    2399    3219 
   Size:       1863     536     820

  Fragments arranged by size: 

                1863     820     536
 
AccI GT'mk_AC

Cuts at:      0    1706    3219 
   Size:       1706    1513
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     214    2259    3219 
   Size:         70     144    2045     960

  Fragments arranged by size: 

                2045     960     144      70
 
AfeI AGC'GCT

Cuts at:      0    2780    3219 
   Size:       2780     439
 
AleI CACnn'nnGTG

Cuts at:      0     785    3219 
   Size:        785    2434
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1609    1641    3219 
   Size:       1609      32    1578

  Fragments arranged by size: 

                1609    1578      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     215    1083    1206    1371    2025    2391    3015    3219 
   Size:        215     868     123     165     654     366     624     204

  Fragments arranged by size: 

                 868     654     624     366     215     204     165     123
            
 
ApaI G_GGCC'C

Cuts at:      0     697    1158    1963    2121    2230    2256    3219 
   Size:        697     461     805     158     109      26     963

  Fragments arranged by size: 

                 963     805     697     461     158     109      26
 
ApaLI G'TGCA_C

Cuts at:      0    1876    3219 
   Size:       1876    1343
 
AscI GG'CGCG_CC

Cuts at:      0    1102    3219 
   Size:       1102    2117
 
AvaI C'yCGr_G

Cuts at:      0     446     697     955    1091    1150    1157    1810    1882 
   Size:        446     251     258     136      59       7     653      72

Cuts at:   1882    2266    2488    2532    2573    2995    3021    3219 
   Size:        384     222      44      41     422      26     198

  Fragments arranged by size: 

                 653     446     422     384     258     251     222     198
                 136      72      59      44      41      26       7
 
BanI G'GyrC_C

Cuts at:      0     139     253    1196    2519    2989    3047    3219 
   Size:        139     114     943    1323     470      58     172

  Fragments arranged by size: 

                1323     943     470     172     139     114      58
 
BanII G_rGCy'C

Cuts at:      0     164     616     664     697     707     950    1040    1158 
   Size:        164     452      48      33      10     243      90     118

Cuts at:   1158    1226    1457    1541    1963    2121    2176    2230    2256 
   Size:         68     231      84     422     158      55      54      26

Cuts at:   2256    2616    2662    3002    3219 
   Size:        360      46     340     217

  Fragments arranged by size: 

                 452     422     360     340     243     231     217     164
                 158     118      90      84      68      55      54      48
                  46      33      26      10
 
BbeI G_GCGC'C

Cuts at:      0     143    1200    2993    3051    3219 
   Size:        143    1057    1793      58     168

  Fragments arranged by size: 

                1793    1057     168     143      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2907    3219 
   Size:       2907     312
 
BbvCI CC'TCA_GC

Cuts at:      0     475    3219 
   Size:        475    2744
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     550     584    3219 
   Size:        550      34    2635

  Fragments arranged by size: 

                2635     550      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3079    3219 
   Size:       3079     140
 
BglI GCCn_nnn'nGGC

Cuts at:      0     356    2152    2657    3098    3219 
   Size:        356    1796     505     441     121

  Fragments arranged by size: 

                1796     505     441     356     121
 
BlpI GC'TnA_GC

Cuts at:      0      94    3219 
   Size:         94    3125
 
Bme1580I G_kGCm'C

Cuts at:      0     697     995    1158    1357    1759    1880    1963    2121 
   Size:        697     298     163     199     402     121      83     158

Cuts at:   2121    2230    2256    2488    2522    2625    3219 
   Size:        109      26     232      34     103     594

  Fragments arranged by size: 

                 697     594     402     298     232     199     163     158
                 121     109     103      83      34      26
 
BmgBI CAC'GTC

Cuts at:      0     342     492    3177    3219 
   Size:        342     150    2685      42

  Fragments arranged by size: 

                2685     342     150      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     658    3219 
   Size:        658    2561
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     635    1476    1908    2630    3219 
   Size:        635     841     432     722     589

  Fragments arranged by size: 

                 841     722     635     589     432
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     243     310     819     964    1234    1252    1432 
   Size:        114     129      67     509     145     270      18     180

Cuts at:   1432    1690    1771    2047    2081    2314    2545    2852    2874 
   Size:        258      81     276      34     233     231     307      22

Cuts at:   2874    3094    3219 
   Size:        220     125

  Fragments arranged by size: 

                 509     307     276     270     258     233     231     220
                 180     145     129     125     114      81      67      34
                  22      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     475     658    1969    2729    3219 
   Size:        475     183    1311     760     490

  Fragments arranged by size: 

                1311     760     490     475     183
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1360    2625    3219 
   Size:       1360    1265     594

  Fragments arranged by size: 

                1360    1265     594
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     643    3219 
   Size:        643    2576
 
BsaAI yAC'GTr

Cuts at:      0     330    2505    3219 
   Size:        330    2175     714

  Fragments arranged by size: 

                2175     714     330
 
BsaHI Gr'CG_yC

Cuts at:      0     140    1197    2990    3048    3219 
   Size:        140    1057    1793      58     171

  Fragments arranged by size: 

                1793    1057     171     140      58
 
BsaWI w'CCGG_w

Cuts at:      0    2829    3219 
   Size:       2829     390
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     103     133    1012    1042    1699    1729    3219 
   Size:        103      30     879      30     657      30    1490

  Fragments arranged by size: 

                1490     879     657     103      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     511     608     715    1603    1666    1687    1828    1939 
   Size:        511      97     107     888      63      21     141     111

Cuts at:   1939    2872    3219 
   Size:        933     347

  Fragments arranged by size: 

                 933     888     511     347     141     111     107      97
                  63      21
 
BseYI C'CCAG_C

Cuts at:      0     170     364     844    1076    2120    2175    2329    2474 
   Size:        170     194     480     232    1044      55     154     145

Cuts at:   2474    2565    2710    2949    3057    3219 
   Size:         91     145     239     108     162

  Fragments arranged by size: 

                1044     480     239     232     194     170     162     154
                 145     145     108      91      55
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     870     994    1617    2073    2170    2843    3219 
   Size:        201     669     124     623     456      97     673     376

  Fragments arranged by size: 

                 673     669     623     456     376     201     124      97
            
 
BsiEI CG_ry'CG

Cuts at:      0    2652    3041    3219 
   Size:       2652     389     178

  Fragments arranged by size: 

                2652     389     178
 
BsiHKAI G_wGCw'C

Cuts at:      0     164     616     798    1541    1880    2069    2239    3219 
   Size:        164     452     182     743     339     189     170     980

  Fragments arranged by size: 

                 980     743     452     339     189     182     170     164
            
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     616     664     697     707     798     950     995 
   Size:        164     452      48      33      10      91     152      45

Cuts at:    995    1040    1158    1226    1357    1457    1541    1759    1880 
   Size:         45     118      68     131     100      84     218     121

Cuts at:   1880    1963    2069    2121    2176    2230    2239    2256    2488 
   Size:         83     106      52      55      54       9      17     232

Cuts at:   2488    2522    2616    2625    2662    3002    3219 
   Size:         34      94       9      37     340     217

  Fragments arranged by size: 

                 452     340     232     218     217     164     152     131
                 121     118     106     100      94      91      84      83
                  68      55      54      52      48      45      45      37
                  34      33      17      10       9       9
 
BspEI T'CCGG_A

Cuts at:      0    2829    3219 
   Size:       2829     390
 
BspHI T'CATG_A

Cuts at:      0    1256    3219 
   Size:       1256    1963
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1031    1055    1863    2108    2399    3219 
   Size:       1031      24     808     245     291     820

  Fragments arranged by size: 

                1031     820     808     291     245      24
 
BsrBI CCG'CTC

Cuts at:      0     294     906    3219 
   Size:        294     612    2313

  Fragments arranged by size: 

                2313     612     294
 
BsrFI r'CCGG_y

Cuts at:      0     250     319     355     807     896    1130    2044    2189 
   Size:        250      69      36     452      89     234     914     145

Cuts at:   2189    3219 
   Size:       1030

  Fragments arranged by size: 

                1030     914     452     250     234     145      89      69
                  36
 
BsrGI T'GTAC_A

Cuts at:      0    1727    3219 
   Size:       1727    1492
 
BssHII G'CGCG_C

Cuts at:      0    1102    3219 
   Size:       1102    2117
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1371    2193    2851    3219 
   Size:       1371     822     658     368

  Fragments arranged by size: 

                1371     822     658     368
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     516    1464    2367    3219 
   Size:        516     948     903     852

  Fragments arranged by size: 

                 948     903     852     516
 
BstYI r'GATC_y

Cuts at:      0    3194    3219 
   Size:       3194      25
 
BtgI C'CryG_G

Cuts at:      0     926    1001    1014    1478    1658    2222    2652    3219 
   Size:        926      75      13     464     180     564     430     567

  Fragments arranged by size: 

                 926     567     564     464     430     180      75      13
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     161     374    3219 
   Size:        161     213    2845

  Fragments arranged by size: 

                2845     213     161
 
BtsI GCAGTG_nn'

Cuts at:      0     882    1705    1930    2469    3219 
   Size:        882     823     225     539     750

  Fragments arranged by size: 

                 882     823     750     539     225
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     611     646    3219 
   Size:        611      35    2573

  Fragments arranged by size: 

                2573     611      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     817    2505    3219 
   Size:        817    1688     714

  Fragments arranged by size: 

                1688     817     714
 
EaeI y'GGCC_r

Cuts at:      0     245     321     918    1334    1466    1643    2105    2219 
   Size:        245      76     597     416     132     177     462     114

Cuts at:   2219    2649    2749    3038    3219 
   Size:        430     100     289     181

  Fragments arranged by size: 

                 597     462     430     416     289     245     181     177
                 132     114     100      76
 
EagI C'GGCC_G

Cuts at:      0    2649    3038    3219 
   Size:       2649     389     181

  Fragments arranged by size: 

                2649     389     181
 
EarI CTCTTCn'nnn_

Cuts at:      0     475    1918    2603    3219 
   Size:        475    1443     685     616

  Fragments arranged by size: 

                1443     685     616     475
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     883    2392    3219 
   Size:        883    1509     827

  Fragments arranged by size: 

                1509     883     827
 
EcoICRI GAG'CTC

Cuts at:      0     162     614    1539    3219 
   Size:        162     452     925    1680

  Fragments arranged by size: 

                1680     925     452     162
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     114     214     243     310     819     964    1234 
   Size:         70      44     100      29      67     509     145     270

Cuts at:   1234    1252    1432    1690    1771    2047    2081    2259    2314 
   Size:         18     180     258      81     276      34     178      55

Cuts at:   2314    2545    2852    2874    3094    3219 
   Size:        231     307      22     220     125

  Fragments arranged by size: 

                 509     307     276     270     258     231     220     180
                 178     145     125     100      81      70      67      55
                  44      34      29      22      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     760    2854    3219 
   Size:        760    2094     365

  Fragments arranged by size: 

                2094     760     365
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111     693     694    1020    1062    1154    1959    1960 
   Size:        111     582       1     326      42      92     805       1

Cuts at:   1960    2117    2227    2252    2468    2529    2556    2628    2739 
   Size:        157     110      25     216      61      27      72     111

Cuts at:   2739    2938    3219 
   Size:        199     281

  Fragments arranged by size: 

                 805     582     326     281     216     199     157     111
                 111     110      92      72      61      42      27      25
                   1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     438    3219 
   Size:        438    2781
 
FseI GG_CCGG'CC

Cuts at:      0     323    3219 
   Size:        323    2896
 
HaeII r_GCGC'y

Cuts at:      0     143     266     289    1200    1779    2045    2782    2993 
   Size:        143     123      23     911     579     266     737     211

Cuts at:   2993    3051    3219 
   Size:         58     168

  Fragments arranged by size: 

                 911     737     579     266     211     168     143     123
                  58      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135    1012    1044    1774    1806    3219 
   Size:        103      32     877      32     730      32    1413

  Fragments arranged by size: 

                1413     877     730     103      32      32      32
 
HincII GTy'rAC

Cuts at:      0     975    3219 
   Size:        975    2244
 
KasI G'GCGC_C

Cuts at:      0     139    1196    2989    3047    3219 
   Size:        139    1057    1793      58     172

  Fragments arranged by size: 

                1793    1057     172     139      58
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    3197    3219 
   Size:        467    2730      22

  Fragments arranged by size: 

                2730     467      22
 
MscI TGG'CCA

Cuts at:      0     247    1468    2107    2751    3219 
   Size:        247    1221     639     644     468

  Fragments arranged by size: 

                1221     644     639     468     247
 
MslI CAynn'nnrTG

Cuts at:      0     785    1663    2131    3219 
   Size:        785     878     468    1088

  Fragments arranged by size: 

                1088     878     785     468
 
MspA1I CmG'CkG

Cuts at:      0      66     174     372     564     682     826     917     928 
   Size:         66     108     198     192     118     144      91      11

Cuts at:    928    1206    1472    1596    1869    2184    2211    2810    3037 
   Size:        278     266     124     273     315      27     599     227

Cuts at:   3037    3219 
   Size:        182

  Fragments arranged by size: 

                 599     315     278     273     266     227     198     192
                 182     144     124     118     108      91      66      27
                  11
 
NaeI GCC'GGC

Cuts at:      0     321     357     898    1132    2046    3219 
   Size:        321      36     541     234     914    1173

  Fragments arranged by size: 

                1173     914     541     321     234      36
 
NarI GG'CG_CC

Cuts at:      0     140    1197    2990    3048    3219 
   Size:        140    1057    1793      58     171

  Fragments arranged by size: 

                1793    1057     171     140      58
 
NcoI C'CATG_G

Cuts at:      0    1478    1658    2222    3219 
   Size:       1478     180     564     997

  Fragments arranged by size: 

                1478     997     564     180
 
NgoMIV G'CCGG_C

Cuts at:      0     319     355     896    1130    2044    3219 
   Size:        319      36     541     234     914    1175

  Fragments arranged by size: 

                1175     914     541     319     234      36
 
NruI TCG'CGA

Cuts at:      0    3166    3219 
   Size:       3166      53
 
NspI r_CATG'y

Cuts at:      0      81    1578    3219 
   Size:         81    1497    1641

  Fragments arranged by size: 

                1641    1497      81
 
PasI CC'CwG_GG

Cuts at:      0     597     690    1032    2114    2552    2618    3219 
   Size:        597      93     342    1082     438      66     601

  Fragments arranged by size: 

                1082     601     597     438     342      93      66
 
PfoI T'CCnGG_A

Cuts at:      0      85     428     460     908     941    1211    1448    1748 
   Size:         85     343      32     448      33     270     237     300

Cuts at:   1748    1924    2024    3071    3219 
   Size:        176     100    1047     148

  Fragments arranged by size: 

                1047     448     343     300     270     237     176     148
                 100      85      33      32
 
PmlI CAC'GTG

Cuts at:      0    2505    3219 
   Size:       2505     714
 
PpuMI rG'GwC_Cy

Cuts at:      0     111    1020    1062    2556    2739    3219 
   Size:        111     909      42    1494     183     480

  Fragments arranged by size: 

                1494     909     480     183     111      42
 
PspOMI G'GGCC_C

Cuts at:      0     693    1154    1959    2117    2226    2252    3219 
   Size:        693     461     805     158     109      26     967

  Fragments arranged by size: 

                 967     805     693     461     158     109      26
 
PstI C_TGCA'G

Cuts at:      0     563     764    1250    1874    2018    2847    3219 
   Size:        563     201     486     624     144     829     372

  Fragments arranged by size: 

                 829     624     563     486     372     201     144
 
PvuII CAG'CTG

Cuts at:      0      66     174    1206    1472    1596    1869    2184    2211 
   Size:         66     108    1032     266     124     273     315      27

Cuts at:   2211    3219 
   Size:       1008

  Fragments arranged by size: 

                1032    1008     315     273     266     124     108      66
                  27
 
SacI G_AGCT'C

Cuts at:      0     164     616    1541    3219 
   Size:        164     452     925    1678

  Fragments arranged by size: 

                1678     925     452     164
 
SacII CC_GC'GG

Cuts at:      0     929    3219 
   Size:        929    2290
 
SanDI GG'GwC_CC

Cuts at:      0    2556    2739    3219 
   Size:       2556     183     480

  Fragments arranged by size: 

                2556     480     183
 
SfcI C'TryA_G

Cuts at:      0     559     760     768    1246    1870    2014    2843    3219 
   Size:        559     201       8     478     624     144     829     376

  Fragments arranged by size: 

                 829     624     559     478     376     201     144       8
            
 
SfoI GGC'GCC

Cuts at:      0     141    1198    2991    3049    3219 
   Size:        141    1057    1793      58     170

  Fragments arranged by size: 

                1793    1057     170     141      58
 
SmaI CCC'GGG

Cuts at:      0     957    1093    1159    2575    2997    3023    3219 
   Size:        957     136      66    1416     422      26     196

  Fragments arranged by size: 

                1416     957     422     196     136      66      26
 
SmlI C'TyrA_G

Cuts at:      0    1375    2604    3219 
   Size:       1375    1229     615

  Fragments arranged by size: 

                1375    1229     615
 
SphI G_CATG'C

Cuts at:      0      81    3219 
   Size:         81    3138
 
SrfI GCCC'GGGC

Cuts at:      0    1093    3219 
   Size:       1093    2126
 
StuI AGG'CCT

Cuts at:      0    2002    2272    2457    3219 
   Size:       2002     270     185     762

  Fragments arranged by size: 

                2002     762     270     185
 
StyI C'CwwG_G

Cuts at:      0     509    1097    1478    1658    2222    3102    3219 
   Size:        509     588     381     180     564     880     117

  Fragments arranged by size: 

                 880     588     564     509     381     180     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184    1354    2127    2331    3219 
   Size:        184    1170     773     204     888

  Fragments arranged by size: 

                1170     888     773     204     184
 
TatI w'GTAC_w

Cuts at:      0    1544    1727    3219 
   Size:       1544     183    1492

  Fragments arranged by size: 

                1544    1492     183
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2306    2338    3219 
   Size:       2306      32     881

  Fragments arranged by size: 

                2306     881      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     211    3219 
   Size:        211    3008
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     781    1476    1485    1665    3219 
   Size:        781     695       9     180    1554

  Fragments arranged by size: 

                1554     781     695     180       9
 
XmaI C'CCGG_G

Cuts at:      0     955    1091    1157    2573    2995    3021    3219 
   Size:        955     136      66    1416     422      26     198

  Fragments arranged by size: 

                1416     955     422     198     136      66      26
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AleI     AloI    AlwNI     ApaI
    ApaLI     AscI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstXI    BstYI
     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FseI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NruI     NspI     PasI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SfcI
     SfoI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AlfI
     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI     BclI    BfrBI
    BglII     BmtI    BsaBI    BsiWI     BsmI    BsmBI    BsrDI    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     ClaI     DraI     DrdI    EcoRI
    EcoRV     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     NsiI     PacI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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