Restriction map for pF1KA1777
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# NO CUT ENZYMES (  104 enzymes) #
##################################

AccIII      AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     AscI        AseI        AsnI        
AspEI       AvrII       BbeI        BbrPI       BbvCI       
BciVI       BfrI        BlnI        BlpI        Bpu1102I    
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       FseI        HgaI        HincII      HindII      
HindIII     HpaI        KasI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TthHB8I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        1222  GACGTC               GACGT^C
Acc65I        412  GGTACC               G^GTACC
AccI          730  GTCTAC               GT^[AC][GT]AC
AclI          806  AACGTT               AA^CGTT
AcyI         1222  GACGTC               G[GA]^CG[CT]C
Alw44I        978  GTGCAC               G^TGCAC
ApaI          367  GGGCCC               GGGCC^C
ApaLI         978  GTGCAC               G^TGCAC
Asp700I       103  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       412  GGTACC               G^GTACC
AspI         1392  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         440  TGCGCA               TGC^GCA
BalI         1629  TGGCCA               TGG^CCA
BamHI         968  GGATCC               G^GATCC
BcgI          646  GCACGGCTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          632  TGATCA               T^GATCA
BglI         1150  GCCGTCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1323  AGATCT               A^GATCT
BsaAI         733  TACGTG               [CT]AC^GT[GA]
BsaBI        2719  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1222  GACGTC               G[GA]^CG[CT]C
BsmBI         276  CGTCTC               CGTCTC(1/5)
BsrFI        2330  ACCGGC               [GA]^CCGG[CT]
Cfr10I       2330  ACCGGC               [GA]^CCGG[CT]
DraI         1887  TTTAAA               TTT^AAA
DraIII       1685  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I      1546  ATGCAT               ATGCA^T
FbaI          632  TGATCA               T^GATCA
FspI          440  TGCGCA               TGC^GCA
HaeII        1643  GGCGCT               [GA]GCGC^[CT]
Hin1I        1222  GACGTC               G[GA]^CG[CT]C
KpnI          412  GGTACC               GGTAC^C
MamI         2719  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1629  TGGCCA               TGG^CCA
MscI         1629  TGGCCA               TGG^CCA
NsiI         1546  ATGCAT               ATGCA^T
NspI         2078  GCATGT               [GA]CATG^[CT]
PflFI        1392  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         387  CCAGTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      806  AACGTT               AA^CGTT
PvuII        2386  CAGCTG               CAG^CTG
SapI          574  GAAGAGC              GCTCTTC(1/4)
SmlI         2106  CTTGAG               C^T[CT][GA]AG
StuI          431  AGGCCT               AGG^CCT
Tth111I      1392  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        387  CCAGTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1203  CCAGAGTGACTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          103  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccII         319  CGCG                 CG^CG
             2314  CGCG                
BbsI          750  GTCTTC               GAAGAC(2/6)
             2231  GTCTTC              
BpuAI         750  GTCTTC               GAAGAC(2/6)
             2231  GTCTTC              
BsmAI         286  GAGAC                GTCTC(1/5)
              277  GTCTC               
BsmI         1110  GAATGC               GAATGC(1/-1)
             2405  GCATTC              
BspHI        1083  TCATGA               T^CATGA
             1388  TCATGA              
BsrDI        1122  CATTGC               GCAATG(2/0)
              776  GCAATG              
BstAPI       2334  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2396  GCAAAATCTGC         
BstUI         319  CGCG                 CG^CG
             2314  CGCG                
EcoRI        1305  GAATTC               G^AATTC
             1707  GAATTC              
FauI         1845  CCCGC                CCCGC(4/6)
             2312  CCCGC               
MvnI          319  CGCG                 CG^CG
             2314  CGCG                
NcoI          363  CCATGG               C^CATGG
             1518  CCATGG              
PpuMI        1367  AGGACCC              [GA]G^G[AT]CC[CT]
             2005  GGGACCT             
PstI          942  CTGCAG               CTGCA^G
             1363  CTGCAG              
RcaI         1083  TCATGA               T^CATGA
             1388  TCATGA              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
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