Restriction map for pF1KA1777
 (Linear) (Six-base) MAPSORT of: KIAA1777.seq  Check: 2157  from: 1  to: 2844



KIAA1777.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     682    2844 
   Size:        682    2162
 
AatII G_ACGT'C

Cuts at:      0    1226    2844 
   Size:       1226    1618
 
AccI GT'mk_AC

Cuts at:      0     731    2844 
   Size:        731    2113
 
Acc65I G'GTAC_C

Cuts at:      0     412    2844 
   Size:        412    2432
 
AclI AA'CG_TT

Cuts at:      0     807    2844 
   Size:        807    2037
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302    1808    2077    2218    2844 
   Size:        302    1506     269     141     626

  Fragments arranged by size: 

                1506     626     302     269     141
 
AleI CACnn'nnGTG

Cuts at:      0     204     724    2844 
   Size:        204     520    2120

  Fragments arranged by size: 

                2120     520     204
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     222    2844 
   Size:        222    2622
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     476     508    1736    1768    2290    2322    2420    2452 
   Size:        476      32    1228      32     522      32      98      32

Cuts at:   2452    2844 
   Size:        392

  Fragments arranged by size: 

                1228     522     476     392      98      32      32      32
                  32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      91     133    2187    2340    2844 
   Size:         91      42    2054     153     504

  Fragments arranged by size: 

                2054     504     153      91      42
 
ApaI G_GGCC'C

Cuts at:      0     371    2844 
   Size:        371    2473
 
ApaLI G'TGCA_C

Cuts at:      0     978    2844 
   Size:        978    1866
 
ApoI r'AATT_y

Cuts at:      0    1009    1305    1707    2540    2643    2844 
   Size:       1009     296     402     833     103     201

  Fragments arranged by size: 

                1009     833     402     296     201     103
 
AvaI C'yCGr_G

Cuts at:      0     370     993    1134    2844 
   Size:        370     623     141    1710

  Fragments arranged by size: 

                1710     623     370     141
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     396     429    2844 
   Size:        396      33    2415

  Fragments arranged by size: 

                2415     396      33
 
BamHI G'GATC_C

Cuts at:      0     968    2844 
   Size:        968    1876
 
BanI G'GyrC_C

Cuts at:      0     383     412    1690    2844 
   Size:        383      29    1278    1154

  Fragments arranged by size: 

                1278    1154     383      29
 
BanII G_rGCy'C

Cuts at:      0     304     371     581    1388    1447    2844 
   Size:        304      67     210     807      59    1397

  Fragments arranged by size: 

                1397     807     304     210      67      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     743    2224    2844 
   Size:        743    1481     620

  Fragments arranged by size: 

                1481     743     620
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     635     669    2844 
   Size:        635      34    2175

  Fragments arranged by size: 

                2175     635      34
 
BclI T'GATC_A

Cuts at:      0     632    2844 
   Size:        632    2212
 
BfrBI ATG'CAT

Cuts at:      0    1548    2844 
   Size:       1548    1296
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1156    2844 
   Size:       1156    1688
 
BglII A'GATC_T

Cuts at:      0    1323    2844 
   Size:       1323    1521
 
Bme1580I G_kGCm'C

Cuts at:      0     371     982    2748    2844 
   Size:        371     611    1766      96

  Fragments arranged by size: 

                1766     611     371      96
 
BmgBI CAC'GTC

Cuts at:      0     276    2844 
   Size:        276    2568
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     294    2844 
   Size:        294    2550
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1738    1790    2042    2289    2380    2844 
   Size:       1738      52     252     247      91     464

  Fragments arranged by size: 

                1738     464     252     247      91      52
 
Bpu10I CC'TnA_GC

Cuts at:      0     303    2844 
   Size:        303    2541
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2127    2844 
   Size:       2127     717
 
BsaAI yAC'GTr

Cuts at:      0     735    2844 
   Size:        735    2109
 
BsaBI GATnn'nnATC

Cuts at:      0    2723    2844 
   Size:       2723     121
 
BsaHI Gr'CG_yC

Cuts at:      0    1223    2844 
   Size:       1223    1621
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      55      85    1191    1221    1736    1766    2420    2450 
   Size:         55      30    1106      30     515      30     654      30

Cuts at:   2450    2844 
   Size:        394

  Fragments arranged by size: 

                1106     654     515     394      55      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     717     824    1717    2844 
   Size:        172     545     107     893    1127

  Fragments arranged by size: 

                1127     893     545     172     107
 
BseYI C'CCAG_C

Cuts at:      0      40     828     914    1747    2384    2798    2844 
   Size:         40     788      86     833     637     414      46

  Fragments arranged by size: 

                 833     788     637     414      86      46      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502     804     870     892    1029    1874    2326    2343 
   Size:        502     302      66      22     137     845     452      17

Cuts at:   2343    2464    2498    2844 
   Size:        121      34     346

  Fragments arranged by size: 

                 845     502     452     346     302     137     121      66
                  34      22      17
 
BsiHKAI G_wGCw'C

Cuts at:      0     275     304     982    1388    1447    1778    1991    2844 
   Size:        275      29     678     406      59     331     213     853

  Fragments arranged by size: 

                 853     678     406     331     275     213      59      29
            
 
BsmI GAATG_Cn'

Cuts at:      0    1116    2405    2844 
   Size:       1116    1289     439

  Fragments arranged by size: 

                1289    1116     439
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     282    2844 
   Size:        282    2562
 
Bsp1286I G_dGCh'C

Cuts at:      0     275     304     371     581     982    1388    1447    1778 
   Size:        275      29      67     210     401     406      59     331

Cuts at:   1778    1991    2748    2844 
   Size:        213     757      96

  Fragments arranged by size: 

                 757     406     401     331     275     213     210      96
                  67      59      29
 
BspHI T'CATG_A

Cuts at:      0    1083    1388    2844 
   Size:       1083     305    1456

  Fragments arranged by size: 

                1456    1083     305
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     682     826     892    2844 
   Size:        682     144      66    1952

  Fragments arranged by size: 

                1952     682     144      66
 
BsrDI GCAATG_nn'

Cuts at:      0     783    1121    2844 
   Size:        783     338    1723

  Fragments arranged by size: 

                1723     783     338
 
BsrFI r'CCGG_y

Cuts at:      0    2330    2844 
   Size:       2330     514
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2340    2402    2844 
   Size:       2340      62     442

  Fragments arranged by size: 

                2340     442      62
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     693     726    1762    2267    2844 
   Size:        693      33    1036     505     577

  Fragments arranged by size: 

                1036     693     577     505      33
 
BstYI r'GATC_y

Cuts at:      0     968    1323    2432    2844 
   Size:        968     355    1109     412

  Fragments arranged by size: 

                1109     968     412     355
 
BtgI C'CryG_G

Cuts at:      0     363    1488    1518    2844 
   Size:        363    1125      30    1326

  Fragments arranged by size: 

                1326    1125     363      30
 
BtsI GCAGTG_nn'

Cuts at:      0     139     519    2285    2844 
   Size:        139     380    1766     559

  Fragments arranged by size: 

                1766     559     380     139
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1812    1847    2844 
   Size:       1812      35     997

  Fragments arranged by size: 

                1812     997      35
 
DraI TTT'AAA

Cuts at:      0    1889    2844 
   Size:       1889     955
 
DraIII CAC_nnn'GTG

Cuts at:      0    1690    2844 
   Size:       1690    1154
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     765    1358    1816    2844 
   Size:        765     593     458    1028

  Fragments arranged by size: 

                1028     765     593     458
 
EaeI y'GGCC_r

Cuts at:      0     716    1157    1629    2844 
   Size:        716     441     472    1215

  Fragments arranged by size: 

                1215     716     472     441
 
EarI CTCTTCn'nnn_

Cuts at:      0     278     569    1085    1239    1888    2747    2844 
   Size:        278     291     516     154     649     859      97

  Fragments arranged by size: 

                 859     649     516     291     278     154      97
 
EcoICRI GAG'CTC

Cuts at:      0     302    1386    1445    2844 
   Size:        302    1084      59    1399

  Fragments arranged by size: 

                1399    1084     302      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302    1738    1790    1808    2042    2077    2218    2289 
   Size:        302    1436      52      18     234      35     141      71

Cuts at:   2289    2380    2844 
   Size:         91     464

  Fragments arranged by size: 

                1436     464     302     234     141      91      71      52
                  35      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     848    1368    2006    2844 
   Size:        848     520     638     838

  Fragments arranged by size: 

                 848     838     638     520
 
EcoRI G'AATT_C

Cuts at:      0    1305    1707    2844 
   Size:       1305     402    1137

  Fragments arranged by size: 

                1305    1137     402
 
FspI TGC'GCA

Cuts at:      0     442    2844 
   Size:        442    2402
 
FspAI rTGC'GCAy

Cuts at:      0     442    2844 
   Size:        442    2402
 
HaeII r_GCGC'y

Cuts at:      0    1647    2844 
   Size:       1647    1197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     129     161     286     318     850     882 
   Size:         55      32      42      32     125      32     532      32

Cuts at:    882    2015    2047    2462    2494    2716    2748    2844 
   Size:       1133      32     415      32     222      32      96

  Fragments arranged by size: 

                1133     532     415     222     125      96      55      42
                  32      32      32      32      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     416    2844 
   Size:        416    2428
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41    1400    2844 
   Size:         41    1359    1444

  Fragments arranged by size: 

                1444    1359      41
 
MscI TGG'CCA

Cuts at:      0    1631    2844 
   Size:       1631    1213
 
MslI CAynn'nnrTG

Cuts at:      0     204     676     724    1429    2174    2714    2720    2844 
   Size:        204     472      48     705     745     540       6     124

  Fragments arranged by size: 

                 745     705     540     472     204     124      48       6
            
 
MspA1I CmG'CkG

Cuts at:      0    1349    2388    2562    2844 
   Size:       1349    1039     174     282

  Fragments arranged by size: 

                1349    1039     282     174
 
NcoI C'CATG_G

Cuts at:      0     363    1518    2844 
   Size:        363    1155    1326

  Fragments arranged by size: 

                1326    1155     363
 
NsiI A_TGCA'T

Cuts at:      0    1550    2844 
   Size:       1550    1294
 
NspI r_CATG'y

Cuts at:      0    2082    2844 
   Size:       2082     762
 
PasI CC'CwG_GG

Cuts at:      0    1461    2844 
   Size:       1461    1383
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     393    2844 
   Size:        393    2451
 
PfoI T'CCnGG_A

Cuts at:      0     754     811     971    1504    1991    2186    2844 
   Size:        754      57     160     533     487     195     658

  Fragments arranged by size: 

                 754     658     533     487     195     160      57
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1736    1768    2420    2452    2844 
   Size:       1736      32     652      32     392

  Fragments arranged by size: 

                1736     652     392      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1368    2006    2844 
   Size:       1368     638     838

  Fragments arranged by size: 

                1368     838     638
 
PspOMI G'GGCC_C

Cuts at:      0     367    2844 
   Size:        367    2477
 
PstI C_TGCA'G

Cuts at:      0     946    1367    2844 
   Size:        946     421    1477

  Fragments arranged by size: 

                1477     946     421
 
PvuII CAG'CTG

Cuts at:      0    2388    2844 
   Size:       2388     456
 
SacI G_AGCT'C

Cuts at:      0     304    1388    1447    2844 
   Size:        304    1084      59    1397

  Fragments arranged by size: 

                1397    1084     304      59
 
SapI GCTCTTCn'nnn_

Cuts at:      0     569    2844 
   Size:        569    2275
 
SfcI C'TryA_G

Cuts at:      0     942    1363    2823    2844 
   Size:        942     421    1460      21

  Fragments arranged by size: 

                1460     942     421      21
 
SmlI C'TyrA_G

Cuts at:      0    2106    2844 
   Size:       2106     738
 
StuI AGG'CCT

Cuts at:      0     433    2844 
   Size:        433    2411
 
StyI C'CwwG_G

Cuts at:      0     363    1518    1737    2844 
   Size:        363    1155     219    1107

  Fragments arranged by size: 

                1155    1107     363     219
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1132    2398    2844 
   Size:       1132    1266     446

  Fragments arranged by size: 

                1266    1132     446
 
TatI w'GTAC_w

Cuts at:      0     513    1130    1540    2844 
   Size:        513     617     410    1304

  Fragments arranged by size: 

                1304     617     513     410
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     367     399     854     886    1191    1223    1605    1637 
   Size:        367      32     455      32     305      32     382      32

Cuts at:   1637    1785    1817    2844 
   Size:        148      32    1027

  Fragments arranged by size: 

                1027     455     382     367     305     148      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1395    2844 
   Size:       1395    1449
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1210    2844 
   Size:       1210    1634
 
XmnI GAAnn'nnTTC

Cuts at:      0     107    2844 
   Size:        107    2737
 
ZraI GAC'GTC

Cuts at:      0    1224    2844 
   Size:       1224    1620
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI     BcgI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI
     BtgI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    FspAI    HaeII    Hin4I
     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI     SapI     SfcI     SmlI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII   AflIII     AgeI     AhdI     AscI     AseI    AsiSI
    AvrII     BbeI    BbvCI    BciVI     BlpI     BmrI     BmtI     BsaI
    BsaWI    BsiEI    BsiWI    BspEI    BsrBI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     EagI     EciI
    EcoNI    EcoRV     FalI     FseI   HincII  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
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