Restriction map for pF1KA1411
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AocI        Aor51HI     
ApaI        AscI        Asp718I     AspI        AvaI        
AvrII       BalI        BanII       BbeI        BbrPI       
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      BstAPI      
BstBI       BstEII      BstPI       BstUI       Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DsaI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     FseI        HaeII       
HapII       HgaI        Hin1I       HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MluI        MluNI       MroI        MscI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         1404  GTATAC               GT^[AC][GT]AC
AseI         1524  ATTAAT               AT^TAAT
AsnI         1524  ATTAAT               AT^TAAT
AviII         921  TGCGCA               TGC^GCA
BanI         1387  GGTGCC               G^G[CT][GA]CC
BbvCI        2413  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2627  CGACTGATTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2832  GGATAC               GTATCC(6/5)
BclI         1932  TGATCA               T^GATCA
BsaBI        1195  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         4117  GGTCTC               GGTCTC(1/5)
BseRI        3256  GAGGAG               GAGGAG(10/8)
BspHI         186  TCATGA               T^CATGA
BssSI        3476  CACGAG               CACGAG(-5/-1)
Bst1107I     1404  GTATAC               GTA^TAC
BstXI        1307  CCAGTAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1404  GTATAC               GTA^TAC
CfoI          922  GCGC                 GCG^C
DraIII        728  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1697  GACAGACAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1855  GATATC               GAT^ATC
FauI         4298  CCCGC                CCCGC(4/6)
FbaI         1932  TGATCA               T^GATCA
FspI          921  TGCGCA               TGC^GCA
HhaI          922  GCGC                 GCG^C
HinP1I        922  GCGC                 G^CGC
MamI         1195  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1967  CAATTG               C^AATTG
MunI         1967  CAATTG               C^AATTG
NdeI         2282  CATATG               CA^TATG
PflMI        1394  CCAGTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1524  ATTAAT               AT^TAAT
PstI         1152  CTGCAG               CTGCA^G
RcaI          186  TCATGA               T^CATGA
SapI         3262  GAAGAGC              GCTCTTC(1/4)
SmlI          310  CTTGAG               C^T[CT][GA]AG
SphI          147  GCATGC               GCATG^C
StuI          438  AGGCCT               AGG^CCT
Van91I       1394  CCAGTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2293  TCTAGA               T^CTAGA
XcmI         2819  CCAAACCTAACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI         1130  CCGC                 CCGC(-3/-1)
             4299  CCGC                
AflIII       3160  ACATGT               A^C[GA][CT]GT
             4168  ACATGT              
AhdI         3897  GACTGATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4330  GACAAACAGTC         
Alw44I       1125  GTGCAC               G^TGCAC
             3760  GTGCAC              
AlwNI         958  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1247  CAGTTACTG           
ApaLI        1125  GTGCAC               G^TGCAC
             3760  GTGCAC              
Asp700I       790  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1771  GAAAATGTTC          
AspEI        3897  GACTGATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4330  GACAAACAGTC         
BamHI        1650  GGATCC               G^GATCC
             4261  GGATCC              
BbsI          558  GAAGAC               GAAGAC(2/6)
             1255  GAAGAC              
BpuAI         558  GAAGAC               GAAGAC(2/6)
             1255  GAAGAC              
BsmFI         954  GGGAC                GGGAC(10/14)
             2884  GGGAC               
Bsp1407I      224  TGTACA               T^GTACA
             3665  TGTACA              
BspLU11I     3160  ACATGT               A^CATGT
             4168  ACATGT              
BspMI        1380  ACCTGC               ACCTGC(4/8)
             2208  ACCTGC              
BsrGI         224  TGTACA               T^GTACA
             3665  TGTACA              
DraII        2028  AGGACCT              [GA]G^G[ACGT]CC[CT]
             2385  AGGTCCT             
Eam1105I     3897  GACTGATCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4330  GACAAACAGTC         
EcoO109I     2028  AGGACCT              [GA]G^G[ACGT]CC[CT]
             2385  AGGTCCT             
EcoT22I       506  ATGCAT               ATGCA^T
             4430  ATGCAT              
HaeIII        439  GGCC                 GG^CC
             4016  GGCC                
MspA1I       4162  CAGCTG               C[AC]G^C[GT]G
             4445  CAGCTG              
NheI          747  GCTAGC               G^CTAGC
             1717  GCTAGC              
NsiI          506  ATGCAT               ATGCA^T
             4430  ATGCAT              
PpuMI        2028  AGGACCT              [GA]G^G[AT]CC[CT]
             2385  AGGTCCT             
PvuII        4162  CAGCTG               CAG^CTG
             4445  CAGCTG              
ScaI         3088  AGTACT               AGT^ACT
             3547  AGTACT              
SspBI         224  TGTACA               T^GTACA
             3665  TGTACA              
Tsp45I       2547  GTCAC                ^GT[GC]AC
             3404  GTGAC               
XmnI          790  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1771  GAAAATGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AspHI       AvaII       BbvI        BfaI        BglII       
BmyI        BpmI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmI        Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HindIII     HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com