Restriction map for pF1KA1411
 (Linear) (Six-base) MAPSORT of: KIAA1411.seq  Check: 5658  from: 1  to: 4545



KIAA1411.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AccI GT'mk_AC

Cuts at:      0    1405    4545 
   Size:       1405    3140
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26    1274    1276    1352    3214    3604    3658    3752 
   Size:         26    1248       2      76    1862     390      54      94

Cuts at:   3752    4545 
   Size:        793

  Fragments arranged by size: 

                1862    1248     793     390      94      76      54      26
                   2
 
AflIII A'CryG_T

Cuts at:      0    3160    4168    4545 
   Size:       3160    1008     377

  Fragments arranged by size: 

                3160    1008     377
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3902    4335    4545 
   Size:       3902     433     210

  Fragments arranged by size: 

                3902     433     210
 
AlwNI CAG_nnn'CTG

Cuts at:      0     963    1252    4545 
   Size:        963     289    3293

  Fragments arranged by size: 

                3293     963     289
 
ApaLI G'TGCA_C

Cuts at:      0    1125    3760    4545 
   Size:       1125    2635     785

  Fragments arranged by size: 

                2635    1125     785
 
ApoI r'AATT_y

Cuts at:      0      25     104     116     218     321    1280    1830    2446 
   Size:         25      79      12     102     103     959     550     616

Cuts at:   2446    2559    2739    2901    3517    4510    4545 
   Size:        113     180     162     616     993      35

  Fragments arranged by size: 

                 993     959     616     616     550     180     162     113
                 103     102      79      35      25      12
 
AseI AT'TA_AT

Cuts at:      0    1525    4545 
   Size:       1525    3020
 
BamHI G'GATC_C

Cuts at:      0    1650    4261    4545 
   Size:       1650    2611     284

  Fragments arranged by size: 

                2611    1650     284
 
BanI G'GyrC_C

Cuts at:      0    1387    4545 
   Size:       1387    3158
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     565    1262    4545 
   Size:        565     697    3283

  Fragments arranged by size: 

                3283     697     565
 
BbvCI CC'TCA_GC

Cuts at:      0    2414    4545 
   Size:       2414    2131
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2616    2650    4545 
   Size:       2616      34    1895

  Fragments arranged by size: 

                2616    1895      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2826    4545 
   Size:       2826    1719
 
BclI T'GATC_A

Cuts at:      0    1932    4545 
   Size:       1932    2613
 
BfrBI ATG'CAT

Cuts at:      0     508    4432    4545 
   Size:        508    3924     113

  Fragments arranged by size: 

                3924     508     113
 
BglII A'GATC_T

Cuts at:      0    1200    3786    4346    4545 
   Size:       1200    2586     560     199

  Fragments arranged by size: 

                2586    1200     560     199
 
Bme1580I G_kGCm'C

Cuts at:      0    1129    3764    4545 
   Size:       1129    2635     781

  Fragments arranged by size: 

                2635    1129     781
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1976    4545 
   Size:       1976    2569
 
BmtI G_CTAG'C

Cuts at:      0     751    1721    4545 
   Size:        751     970    2824

  Fragments arranged by size: 

                2824     970     751
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841    1377    1745    4545 
   Size:        841     536     368    2800

  Fragments arranged by size: 

                2800     841     536     368
 
Bpu10I CC'TnA_GC

Cuts at:      0    2414    4545 
   Size:       2414    2131
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    4545 
   Size:        331    4214
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4123    4545 
   Size:       4123     422
 
BsaBI GATnn'nnATC

Cuts at:      0    1199    4545 
   Size:       1199    3346
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    3271    4545 
   Size:       3271    1274
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403    3455    3803    4454    4545 
   Size:        403    3052     348     651      91

  Fragments arranged by size: 

                3052     651     403     348      91
 
BsiHKAI G_wGCw'C

Cuts at:      0    1016    1129    3764    4104    4545 
   Size:       1016     113    2635     340     441

  Fragments arranged by size: 

                2635    1016     441     340     113
 
BsmI GAATG_Cn'

Cuts at:      0     387    1048    2590    4545 
   Size:        387     661    1542    1955

  Fragments arranged by size: 

                1955    1542     661     387
 
Bsp1286I G_dGCh'C

Cuts at:      0    1016    1129    3764    4104    4545 
   Size:       1016     113    2635     340     441

  Fragments arranged by size: 

                2635    1016     441     340     113
 
BspHI T'CATG_A

Cuts at:      0     186    4545 
   Size:        186    4359
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1389    2217    4545 
   Size:       1389     828    2328

  Fragments arranged by size: 

                2328    1389     828
 
BsrDI GCAATG_nn'

Cuts at:      0      23     683    1082    3186    4432    4545 
   Size:         23     660     399    2104    1246     113

  Fragments arranged by size: 

                2104    1246     660     399     113      23
 
BsrGI T'GTAC_A

Cuts at:      0     224    3665    4545 
   Size:        224    3441     880

  Fragments arranged by size: 

                3441     880     224
 
BssSI C'ACGA_G

Cuts at:      0    3476    4545 
   Size:       3476    1069
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1314    4545 
   Size:       1314    3231
 
BstYI r'GATC_y

Cuts at:      0     339    1200    1650    1992    3786    4261    4346    4545 
   Size:        339     861     450     342    1794     475      85     199

  Fragments arranged by size: 

                1794     861     475     450     342     339     199      85
            
 
BstZ17I GTA'TAC

Cuts at:      0    1406    4545 
   Size:       1406    3139
 
BtsI GCAGTG_nn'

Cuts at:      0    4104    4545 
   Size:       4104     441
 
DraI TTT'AAA

Cuts at:      0     248    4026    4325    4545 
   Size:        248    3778     299     220

  Fragments arranged by size: 

                3778     299     248     220
 
DraIII CAC_nnn'GTG

Cuts at:      0     733    4545 
   Size:        733    3812
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1703    4545 
   Size:       1703    2842
 
EarI CTCTTCn'nnn_

Cuts at:      0     230     785    1166    2777    3257    3713    4545 
   Size:        230     555     381    1611     480     456     832

  Fragments arranged by size: 

                1611     832     555     480     456     381     230
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     841    1274    1276    1352    1377    1745    3214 
   Size:         26     815     433       2      76      25     368    1469

Cuts at:   3214    3604    3658    3752    4545 
   Size:        390      54      94     793

  Fragments arranged by size: 

                1469     815     793     433     390     368      94      76
                  54      26      25       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2029    2386    4545 
   Size:       2029     357    2159

  Fragments arranged by size: 

                2159    2029     357
 
EcoRI G'AATT_C

Cuts at:      0      25     116    1280    4545 
   Size:         25      91    1164    3265

  Fragments arranged by size: 

                3265    1164      91      25
 
EcoRV GAT'ATC

Cuts at:      0    1857    4545 
   Size:       1857    2688
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     325     438     862    2687    3092    3394    3566    3634 
   Size:        325     113     424    1825     405     302     172      68

Cuts at:   3634    4246    4545 
   Size:        612     299

  Fragments arranged by size: 

                1825     612     424     405     325     302     299     172
                 113      68
 
FspI TGC'GCA

Cuts at:      0     923    4545 
   Size:        923    3622
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     600     632    2530    2562    3888    3920    3927    3959 
   Size:        600      32    1898      32    1326      32       7      32

Cuts at:   3959    4321    4353    4545 
   Size:        362      32     192

  Fragments arranged by size: 

                1898    1326     600     362     192      32      32      32
                  32      32       7
 
HindIII A'AGCT_T

Cuts at:      0     540    1486    2144    3963    4210    4545 
   Size:        540     946     658    1819     247     335

  Fragments arranged by size: 

                1819     946     658     540     335     247
 
MfeI C'AATT_G

Cuts at:      0    1967    4545 
   Size:       1967    2578
 
MslI CAynn'nnrTG

Cuts at:      0     449     455    2594    3019    4306    4545 
   Size:        449       6    2139     425    1287     239

  Fragments arranged by size: 

                2139    1287     449     425     239       6
 
MspA1I CmG'CkG

Cuts at:      0    4164    4447    4545 
   Size:       4164     283      98

  Fragments arranged by size: 

                4164     283      98
 
NdeI CA'TA_TG

Cuts at:      0    2283    4545 
   Size:       2283    2262
 
NheI G'CTAG_C

Cuts at:      0     747    1717    4545 
   Size:        747     970    2828

  Fragments arranged by size: 

                2828     970     747
 
NsiI A_TGCA'T

Cuts at:      0     510    4434    4545 
   Size:        510    3924     111

  Fragments arranged by size: 

                3924     510     111
 
NspI r_CATG'y

Cuts at:      0     151    3164    3432    4172    4545 
   Size:        151    3013     268     740     373

  Fragments arranged by size: 

                3013     740     373     268     151
 
PciI A'CATG_T

Cuts at:      0    3160    4168    4545 
   Size:       3160    1008     377

  Fragments arranged by size: 

                3160    1008     377
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1400    4545 
   Size:       1400    3145
 
PpuMI rG'GwC_Cy

Cuts at:      0    2029    2386    4545 
   Size:       2029     357    2159

  Fragments arranged by size: 

                2159    2029     357
 
PsiI TTA'TAA

Cuts at:      0     907     973    2438    4545 
   Size:        907      66    1465    2107

  Fragments arranged by size: 

                2107    1465     907      66
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3626    3658    4545 
   Size:       3626      32     887

  Fragments arranged by size: 

                3626     887      32
 
PstI C_TGCA'G

Cuts at:      0    1156    4545 
   Size:       1156    3389
 
PvuII CAG'CTG

Cuts at:      0    4164    4447    4545 
   Size:       4164     283      98

  Fragments arranged by size: 

                4164     283      98
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3257    4545 
   Size:       3257    1288
 
ScaI AGT'ACT

Cuts at:      0    3090    3549    4545 
   Size:       3090     459     996

  Fragments arranged by size: 

                3090     996     459
 
SfcI C'TryA_G

Cuts at:      0    1117    1152    1343    2120    2720    2879    3038    3174 
   Size:       1117      35     191     777     600     159     159     136

Cuts at:   3174    4267    4545 
   Size:       1093     278

  Fragments arranged by size: 

                1117    1093     777     600     278     191     159     159
                 136      35
 
SmlI C'TyrA_G

Cuts at:      0     310    4545 
   Size:        310    4235
 
SphI G_CATG'C

Cuts at:      0     151    4545 
   Size:        151    4394
 
SspI AAT'ATT

Cuts at:      0     998    3502    4030    4499    4537    4545 
   Size:        998    2504     528     469      38       8

  Fragments arranged by size: 

                2504     998     528     469      38       8
 
StuI AGG'CCT

Cuts at:      0     440    4545 
   Size:        440    4105
 
StyI C'CwwG_G

Cuts at:      0     335    2032    4018    4077    4545 
   Size:        335    1697    1986      59     468

  Fragments arranged by size: 

                1986    1697     468     335      59
 
TatI w'GTAC_w

Cuts at:      0     224    1582    2467    2476    3088    3442    3547    3665 
   Size:        224    1358     885       9     612     354     105     118

Cuts at:   3665    3741    4545 
   Size:         76     804

  Fragments arranged by size: 

                1358     885     804     612     354     224     118     105
                  76       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2824    2856    4545 
   Size:       2824      32    1689

  Fragments arranged by size: 

                2824    1689      32
 
XbaI T'CTAG_A

Cuts at:      0    2293    4545 
   Size:       2293    2252
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2826    4545 
   Size:       2826    1719
 
XmnI GAAnn'nnTTC

Cuts at:      0     794    1775    4545 
   Size:        794     981    2770

  Fragments arranged by size: 

                2770     981     794
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI    AlwNI    ApaLI     ApoI     AseI
    BamHI     BanI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI    BssSI    BstXI    BstYI  BstZ17I     BtsI     DraI
   DraIII     DrdI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    Hin4I  HindIII     MfeI     MslI   MspA1I     NdeI     NheI
     NsiI     NspI     PciI    PflMI    PpuMI     PsiI     PsrI     PstI
    PvuII     SapI     ScaI     SfcI     SmlI     SphI     SspI     StuI
     StyI     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI
    BanII     BbeI     BglI     BlpI    BmgBI     BplI    BsaAI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI
    BsrFI   BssHII   BstAPI    BstBI   BstEII   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     EaeI     EagI     EciI  EcoICRI    EcoNI     FseI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     PacI
     PasI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SwaI    TaqII  Tth111I     XhoI     XmaI     ZraI
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