Restriction map for pF1KA0726
 (Linear) (Six-base) MAPSORT of: KIAA0726.seq  Check: 6918  from: 1  to: 2868



KIAA0726.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     175    1047    2026    2868 
   Size:        175     872     979     842

  Fragments arranged by size: 

                 979     872     842     175
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1836    1891    1901    2868 
   Size:       1836      55      10     967

  Fragments arranged by size: 

                1836     967      55      10
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1548    2868 
   Size:       1548    1320
 
AlwNI CAG_nnn'CTG

Cuts at:      0     775    1722    1929    2457    2868 
   Size:        775     947     207     528     411

  Fragments arranged by size: 

                 947     775     528     411     207
 
ApaI G_GGCC'C

Cuts at:      0     352    1097    2868 
   Size:        352     745    1771

  Fragments arranged by size: 

                1771     745     352
 
ApaLI G'TGCA_C

Cuts at:      0    1000    2104    2868 
   Size:       1000    1104     764

  Fragments arranged by size: 

                1104    1000     764
 
ApoI r'AATT_y

Cuts at:      0    2126    2377    2868 
   Size:       2126     251     491

  Fragments arranged by size: 

                2126     491     251
 
AvaI C'yCGr_G

Cuts at:      0    1360    2155    2868 
   Size:       1360     795     713

  Fragments arranged by size: 

                1360     795     713
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     430     463    2868 
   Size:        430      33    2405

  Fragments arranged by size: 

                2405     430      33
 
BamHI G'GATC_C

Cuts at:      0    2151    2868 
   Size:       2151     717
 
BanI G'GyrC_C

Cuts at:      0     222    1042    1059    1340    2868 
   Size:        222     820      17     281    1528

  Fragments arranged by size: 

                1528     820     281     222      17
 
BanII G_rGCy'C

Cuts at:      0     352    1090    1097    2213    2868 
   Size:        352     738       7    1116     655

  Fragments arranged by size: 

                1116     738     655     352       7
 
BbvCI CC'TCA_GC

Cuts at:      0     695    2868 
   Size:        695    2173
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     977    1011    1383    1417    2868 
   Size:        977      34     372      34    1451

  Fragments arranged by size: 

                1451     977     372      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     811    2868 
   Size:        811    2057
 
BfrBI ATG'CAT

Cuts at:      0    1642    2868 
   Size:       1642    1226
 
BglII A'GATC_T

Cuts at:      0     191     536    2868 
   Size:        191     345    2332

  Fragments arranged by size: 

                2332     345     191
 
BlpI GC'TnA_GC

Cuts at:      0    2440    2868 
   Size:       2440     428
 
Bme1580I G_kGCm'C

Cuts at:      0     227     352    1004    1064    1097    1290    2071    2108 
   Size:        227     125     652      60      33     193     781      37

Cuts at:   2108    2868 
   Size:        760

  Fragments arranged by size: 

                 781     760     652     227     193     125      60      37
                  33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     421     519     947    1070    1288    2868 
   Size:        421      98     428     123     218    1580

  Fragments arranged by size: 

                1580     428     421     218     123      98
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2303    2332    2868 
   Size:       2303      29     536

  Fragments arranged by size: 

                2303     536      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     829    1336    1630    1753    2227    2352    2633    2643 
   Size:        829     507     294     123     474     125     281      10

Cuts at:   2643    2868 
   Size:        225

  Fragments arranged by size: 

                 829     507     474     294     281     225     125     123
                  10
 
Bpu10I CC'TnA_GC

Cuts at:      0     695    1599    2868 
   Size:        695     904    1269

  Fragments arranged by size: 

                1269     904     695
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1300    2868 
   Size:       1300    1568
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     805    1535    1744    2842    2868 
   Size:        805     730     209    1098      26

  Fragments arranged by size: 

                1098     805     730     209      26
 
BsaAI yAC'GTr

Cuts at:      0    1704    1896    2436    2868 
   Size:       1704     192     540     432

  Fragments arranged by size: 

                1704     540     432     192
 
BsaHI Gr'CG_yC

Cuts at:      0    1814    1916    1988    2868 
   Size:       1814     102      72     880

  Fragments arranged by size: 

                1814     880     102      72
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1381    1411    2506    2536    2868 
   Size:       1381      30    1095      30     332

  Fragments arranged by size: 

                1381    1095     332      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1501    2868 
   Size:       1501    1367
 
BseYI C'CCAG_C

Cuts at:      0     730     775    1083    1959    2242    2428    2447    2533 
   Size:        730      45     308     876     283     186      19      86

Cuts at:   2533    2821    2868 
   Size:        288      47

  Fragments arranged by size: 

                 876     730     308     288     283     186      86      47
                  45      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1075    2386    2443    2868 
   Size:       1075    1311      57     425

  Fragments arranged by size: 

                1311    1075     425      57
 
BsiEI CG_ry'CG

Cuts at:      0     483    2868 
   Size:        483    2385
 
BsiHKAI G_wGCw'C

Cuts at:      0    1004    2108    2213    2441    2582    2868 
   Size:       1004    1104     105     228     141     286

  Fragments arranged by size: 

                1104    1004     286     228     141     105
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1875    2634    2829    2868 
   Size:       1875     759     195      39

  Fragments arranged by size: 

                1875     759     195      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     352    1004    1064    1090    1097    1290    2071 
   Size:        227     125     652      60      26       7     193     781

Cuts at:   2071    2108    2213    2441    2582    2868 
   Size:         37     105     228     141     286

  Fragments arranged by size: 

                 781     652     286     228     227     193     141     125
                 105      60      37      26       7
 
BspHI T'CATG_A

Cuts at:      0    1463    2868 
   Size:       1463    1405
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     175    1047    1311    2026    2868 
   Size:        175     872     264     715     842

  Fragments arranged by size: 

                 872     842     715     264     175
 
BsrBI CCG'CTC

Cuts at:      0     906    2160    2868 
   Size:        906    1254     708

  Fragments arranged by size: 

                1254     906     708
 
BsrDI GCAATG_nn'

Cuts at:      0    2379    2868 
   Size:       2379     489
 
BsrFI r'CCGG_y

Cuts at:      0    2638    2868 
   Size:       2638     230
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     691     772    2457    2868 
   Size:        691      81    1685     411

  Fragments arranged by size: 

                1685     691     411      81
 
BstEII G'GTnAC_C

Cuts at:      0    2467    2868 
   Size:       2467     401
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     217     870    1083    1426    2868 
   Size:        217     653     213     343    1442

  Fragments arranged by size: 

                1442     653     343     217     213
 
BstYI r'GATC_y

Cuts at:      0     191     536     948    1925    2151    2273    2868 
   Size:        191     345     412     977     226     122     595

  Fragments arranged by size: 

                 977     595     412     345     226     191     122
 
Bsu36I CC'TnA_GG

Cuts at:      0    2278    2868 
   Size:       2278     590
 
BtgI C'CryG_G

Cuts at:      0      79     210    1242    1569    2463    2723    2868 
   Size:         79     131    1032     327     894     260     145

  Fragments arranged by size: 

                1032     894     327     260     145     131      79
 
BtsI GCAGTG_nn'

Cuts at:      0    1835    1940    2802    2868 
   Size:       1835     105     862      66

  Fragments arranged by size: 

                1835     862     105      66
 
DraIII CAC_nnn'GTG

Cuts at:      0     772    2037    2868 
   Size:        772    1265     831

  Fragments arranged by size: 

                1265     831     772
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     127    2868 
   Size:        127    2741
 
EaeI y'GGCC_r

Cuts at:      0     872    2358    2810    2868 
   Size:        872    1486     452      58

  Fragments arranged by size: 

                1486     872     452      58
 
EarI CTCTTCn'nnn_

Cuts at:      0    1169    1853    2264    2689    2868 
   Size:       1169     684     411     425     179

  Fragments arranged by size: 

                1169     684     425     411     179
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     793    2868 
   Size:        793    2075
 
EcoICRI GAG'CTC

Cuts at:      0    2211    2868 
   Size:       2211     657
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     829    1336    1630    1753    1836    1891    1901    2227 
   Size:        829     507     294     123      83      55      10     326

Cuts at:   2227    2352    2633    2643    2868 
   Size:        125     281      10     225

  Fragments arranged by size: 

                 829     507     326     294     281     225     125     123
                  83      55      10      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     247    2281    2868 
   Size:        247    2034     587

  Fragments arranged by size: 

                2034     587     247
 
EcoO109I rG'GnC_Cy

Cuts at:      0     348     349    1094    1472    1820    2654    2673    2868 
   Size:        348       1     745     378     348     834      19     195

  Fragments arranged by size: 

                 834     745     378     348     348     195      19       1
            
 
EcoRI G'AATT_C

Cuts at:      0    2377    2868 
   Size:       2377     491
 
FspI TGC'GCA

Cuts at:      0    2605    2868 
   Size:       2605     263
 
FspAI rTGC'GCAy

Cuts at:      0    2605    2868 
   Size:       2605     263
 
HaeII r_GCGC'y

Cuts at:      0     913    2540    2868 
   Size:        913    1627     328

  Fragments arranged by size: 

                1627     913     328
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     883     915    1204    1236    1381    1413    1534    1566 
   Size:        883      32     289      32     145      32     121      32

Cuts at:   1566    2170    2202    2828    2860    2868 
   Size:        604      32     626      32       8

  Fragments arranged by size: 

                 883     626     604     289     145     121      32      32
                  32      32      32      32       8
 
HincII GTy'rAC

Cuts at:      0     405    2868 
   Size:        405    2463
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2072    2868 
   Size:       2072     796
 
MscI TGG'CCA

Cuts at:      0     874    2868 
   Size:        874    1994
 
MslI CAynn'nnrTG

Cuts at:      0     109    1424    2086    2260    2554    2557    2713    2868 
   Size:        109    1315     662     174     294       3     156     155

  Fragments arranged by size: 

                1315     662     294     174     156     155     109       3
            
 
MspA1I CmG'CkG

Cuts at:      0     174     734    1963    2199    2868 
   Size:        174     560    1229     236     669

  Fragments arranged by size: 

                1229     669     560     236     174
 
NaeI GCC'GGC

Cuts at:      0    2640    2868 
   Size:       2640     228
 
NcoI C'CATG_G

Cuts at:      0      79     210    1569    2723    2868 
   Size:         79     131    1359    1154     145

  Fragments arranged by size: 

                1359    1154     145     131      79
 
NgoMIV G'CCGG_C

Cuts at:      0    2638    2868 
   Size:       2638     230
 
NsiI A_TGCA'T

Cuts at:      0    1644    2868 
   Size:       1644    1224
 
NspI r_CATG'y

Cuts at:      0     397    1646    1777    2091    2868 
   Size:        397    1249     131     314     777

  Fragments arranged by size: 

                1249     777     397     314     131
 
PasI CC'CwG_GG

Cuts at:      0    1065    2868 
   Size:       1065    1803
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     775    2868 
   Size:        775    2093
 
PfoI T'CCnGG_A

Cuts at:      0    2174    2868 
   Size:       2174     694
 
PmlI CAC'GTG

Cuts at:      0    1704    2436    2868 
   Size:       1704     732     432

  Fragments arranged by size: 

                1704     732     432
 
PpuMI rG'GwC_Cy

Cuts at:      0    2673    2868 
   Size:       2673     195
 
PspOMI G'GGCC_C

Cuts at:      0     348    1093    2868 
   Size:        348     745    1775

  Fragments arranged by size: 

                1775     745     348
 
PspXI vC'TCGA_Gb

Cuts at:      0    1360    2868 
   Size:       1360    1508
 
PstI C_TGCA'G

Cuts at:      0     620     860    1772    2195    2868 
   Size:        620     240     912     423     673

  Fragments arranged by size: 

                 912     673     620     423     240
 
PvuI CG_AT'CG

Cuts at:      0     483    2868 
   Size:        483    2385
 
PvuII CAG'CTG

Cuts at:      0     734    1963    2868 
   Size:        734    1229     905

  Fragments arranged by size: 

                1229     905     734
 
SacI G_AGCT'C

Cuts at:      0    2213    2868 
   Size:       2213     655
 
SexAI A'CCwGG_T

Cuts at:      0     768    2033    2868 
   Size:        768    1265     835

  Fragments arranged by size: 

                1265     835     768
 
SfcI C'TryA_G

Cuts at:      0     616     856    1005    1248    1662    1768    2191    2868 
   Size:        616     240     149     243     414     106     423     677

  Fragments arranged by size: 

                 677     616     423     414     243     240     149     106
            
 
SmlI C'TyrA_G

Cuts at:      0    1279    1360    2868 
   Size:       1279      81    1508

  Fragments arranged by size: 

                1508    1279      81
 
StuI AGG'CCT

Cuts at:      0     331    2868 
   Size:        331    2537
 
StyI C'CwwG_G

Cuts at:      0      79     155     210     278     875    1536    1569    2669 
   Size:         79      76      55      68     597     661      33    1100

Cuts at:   2669    2723    2868 
   Size:         54     145

  Fragments arranged by size: 

                1100     661     597     145      79      76      68      55
                  54      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     576    1057    1060    1454    1549    2868 
   Size:        576     481       3     394      95    1319

  Fragments arranged by size: 

                1319     576     481     394      95       3
 
TatI w'GTAC_w

Cuts at:      0     527     620    1522    2868 
   Size:        527      93     902    1346

  Fragments arranged by size: 

                1346     902     527      93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     909     941     983    1015    2506    2538    2868 
   Size:        909      32      42      32    1491      32     330

  Fragments arranged by size: 

                1491     909     330      42      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285     942    1567    1881    2684    2868 
   Size:        285     657     625     314     803     184

  Fragments arranged by size: 

                 803     657     625     314     285     184
 
XhoI C'TCGA_G

Cuts at:      0    1360    2868 
   Size:       1360    1508
 

 Enzymes that do cut:

     AarI     AcuI     AhdI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BaeI    BamHI     BanI    BanII    BbvCI     BcgI    BciVI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FspI    FspAI    HaeII    Hin4I   HincII     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI     PvuI
    PvuII     SacI    SexAI     SfcI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BbeI
     BbsI     BclI     BglI    BmgBI     BmtI    BsaBI    BsaWI    BsiWI
     BsmI    BspEI    BsrGI   BssHII    BssSI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DraI     EagI    EcoRV     FalI     FseI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI
     NotI     NruI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
     PsrI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XbaI     XmaI     XmnI     ZraI
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