Restriction map for pF1KA0382
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      ApaI        ApaLI       
Asp718I     AspEI       AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BfrI        
BlnI        BlpI        Bpu1102I    BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspEI       BsrFI       BsrGI       BssSI       
Bst1107I    BstEII      BstPI       BstZ17I     CelII       
Cfr10I      CpoI        DraI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRV       EcoT22I     
FseI        FspI        KasI        KpnI        KspI        
MluI        MroI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       SwaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI           15  GTCTAC               GT^[AC][GT]AC
AccII        1871  CGCG                 CG^CG
AcyI          437  GACGCC               G[GA]^CG[CT]C
Aor51HI      4433  AGCGCT               AGC^GCT
AscI         1869  GGCGCGCC             GG^CGCGCC
AseI         2374  ATTAAT               AT^TAAT
AsnI         2374  ATTAAT               AT^TAAT
BalI         2857  TGGCCA               TGG^CCA
BanI         4029  GGCACC               G^G[CT][GA]CC
BbvCI         739  CCTCAGC              CCTCAGC(-5/-2)
BcnI         1143  CCCGG                CC^[GC]GG
BglI          307  GCCATGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        3614  TACGTG               [CT]AC^GT[GA]
BsaHI         437  GACGCC               G[GA]^CG[CT]C
BspLU11I     1549  ACATGT               A^CATGT
BsrBI        1543  GAGCGG               CCGCTC(-3/-3)
BssHII       1870  GCGCGC               G^CGCGC
BstAPI       3745  GCATTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1871  CGCG                 CG^CG
DraIII       4160  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         2857  TGGCCA               [CT]^GGCC[GA]
Eco47III     4433  AGCGCT               AGC^GCT
EcoNI        3445  CCTGGCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2462  GAATTC               G^AATTC
FauI          312  GCGGG                CCCGC(4/6)
HapII        1144  CCGG                 C^CGG
Hin1I         437  GACGCC               G[GA]^CG[CT]C
HindIII      3284  AAGCTT               A^AGCTT
HpaI         4198  GTTAAC               GTT^AAC
HpaII        1144  CCGG                 C^CGG
MfeI         1841  CAATTG               C^AATTG
MluNI        2857  TGGCCA               TGG^CCA
MscI         2857  TGGCCA               TGG^CCA
MspI         1144  CCGG                 C^CGG
MunI         1841  CAATTG               C^AATTG
MvnI         1871  CGCG                 CG^CG
NciI         1143  CCCGG                CC^[GC]GG
PshAI         500  GACATATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2374  ATTAAT               AT^TAAT
SmlI         1267  CTTGAG               C^T[CT][GA]AG
SpeI          565  ACTAGT               A^CTAGT
StuI         3911  AGGCCT               AGG^CCT
XcmI         4584  CCAAAGGCTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AocI         1369  CCTGAGG              CC^T[ACGT]AGG
             1918  CCTCAGG             
AspI          159  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3880  GACAGTGTC           
BciVI        1975  GGATAC               GTATCC(6/5)
             2449  GTATCC              
BclI         2427  TGATCA               T^GATCA
             2690  TGATCA              
BglII        1338  AGATCT               A^GATCT
             2906  AGATCT              
BsaI         1815  GAGACC               GGTCTC(1/5)
             2569  GAGACC              
BspDI        1292  ATCGAT               AT^CGAT
             2581  ATCGAT              
BsrDI         114  CATTGC               GCAATG(2/0)
             3963  CATTGC              
Bsu36I       1369  CCTGAGG              CC^T[ACGT]AGG
             1918  CCTCAGG             
ClaI         1292  ATCGAT               AT^CGAT
             2581  ATCGAT              
DrdI          265  GACAATCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1817  GACCAAGCCGTC        
Eco81I       1369  CCTGAGG              CC^T[ACGT]AGG
             1918  CCTCAGG             
FbaI         2427  TGATCA               T^GATCA
             2690  TGATCA              
HincII       3438  GTCAAC               GT[CT]^[GA]AC
             4198  GTTAAC              
HindII       3438  GTCAAC               GT[CT]^[GA]AC
             4198  GTTAAC              
NcoI         1853  CCATGG               C^CATGG
             4100  CCATGG              
NdeI          502  CATATG               CA^TATG
             3934  CATATG              
NspI         1549  ACATGT               [GA]CATG^[CT]
             2643  ACATGC              
PflFI         159  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3880  GACAGTGTC           
PflMI        4503  CCAGATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4584  CCAAAGGCTGG         
PpuMI        1803  GGGACCC              [GA]G^G[AT]CC[CT]
             3371  AGGACCT             
SapI         3022  GAAGAGC              GCTCTTC(1/4)
             3813  GAAGAGC             
SspI         2836  AATATT               AAT^ATT
             3904  AATATT              
Tth111I       159  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3880  GACAGTGTC           
Van91I       4503  CCAGATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4584  CCAAAGGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BanII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaBI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstBI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HgaI        HhaI        HinP1I      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspV        PleI        PstI        PvuII       RcaI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XbaI        XhoII       XmnI        
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