Restriction map for pF1KB5241
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BcnI        
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
EcoRV       FseI        FspI        HapII       HgaI        
HincII      HindII      HpaI        HpaII       KasI        
KpnI        KspI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        1183  GACGTC               GACGT^C
AccI         1336  GTCTAC               GT^[AC][GT]AC
AcyI         1183  GACGTC               G[GA]^CG[CT]C
AflIII        737  ACATGT               A^C[GA][CT]GT
AocI          615  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       532  AGCGCT               AGC^GCT
ApaI         1385  GGGCCC               GGGCC^C
AspI         1424  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII        1385  GGGCCC               G[GA]GC[CT]^C
BclI           18  TGATCA               T^GATCA
BglII        1305  AGATCT               A^GATCT
BmyI         1385  GGGCCC               G[AGT]GC[ACT]^C
BsaHI        1183  GACGTC               G[GA]^CG[CT]C
BseRI         276  GAGGAG               GAGGAG(10/8)
Bsp1286I     1385  GGGCCC               G[AGT]GC[ACT]^C
BspLU11I      737  ACATGT               A^CATGT
BsrBI        1381  GAGCGG               CCGCTC(-3/-3)
BstEII        285  GGTGACC              G^GT[ACGT]ACC
BstPI         285  GGTGACC              G^GT[ACGT]ACC
BstYI        1305  AGATCT               [GA]^GATC[CT]
Bsu36I        615  CCTAAGG              CC^T[ACGT]AGG
CfoI          533  GCGC                 GCG^C
Eco47III      532  AGCGCT               AGC^GCT
Eco81I        615  CCTAAGG              CC^T[ACGT]AGG
EcoO65I       285  GGTGACC              G^GT[ACGT]ACC
EcoRI         130  GAATTC               G^AATTC
EcoT22I      1101  ATGCAT               ATGCA^T
FauI         1383  GCGGG                CCCGC(4/6)
FbaI           18  TGATCA               T^GATCA
HaeII         532  AGCGCT               [GA]GCGC^[CT]
HhaI          533  GCGC                 GCG^C
Hin1I        1183  GACGTC               G[GA]^CG[CT]C
HinP1I        533  GCGC                 G^CGC
MaeII        1184  ACGT                 A^CGT
MfeI           46  CAATTG               C^AATTG
MflI         1305  AGATCT               [GA]^GATC[CT]
MunI           46  CAATTG               C^AATTG
NsiI         1101  ATGCAT               ATGCA^T
NspI          737  ACATGT               [GA]CATG^[CT]
PflFI        1424  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         640  CCAGACAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1428  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         750  GACCTATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          992  CTGCAG               CTGCA^G
SapI          216  GAAGAGC              GCTCTTC(1/4)
ScaI         1373  AGTACT               AGT^ACT
TaqI          762  TCGA                 T^CGA
Tth111I      1424  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       762  TCGA                 T^CGA
Van91I        640  CCAGACAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1029  TCTAGA               T^CTAGA
XhoII        1305  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI          897  GATCC                GGATC(4/5)
              120  GGATC               
AvaII         510  GGACC                G^G[AT]CC
             1429  GGTCC               
BglI          560  GCCTGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              927  GCCTAGAGGGC         
BpmI           40  CTGGAG               CTGGAG(16/14)
             1369  CTGGAG              
BsaBI          16  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              897  GATCCAAATC          
BsmAI        1392  GAGAC                GTCTC(1/5)
             1422  GAGAC               
DdeI          616  CTAAG                C^T[ACGT]AG
             1390  CTGAG               
DraI          571  TTTAAA               TTT^AAA
             1206  TTTAAA              
DraII        1385  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1428  AGGTCCT             
EarI          216  GAAGAG               CTCTTC(1/4)
             1078  GAAGAG              
EcoNI         351  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              615  CCTAAGGCAGG         
EcoO109I     1385  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             1428  AGGTCCT             
EcoT14I       479  CCAAGG               C^C[AT][AT]GG
             1326  CCTTGG              
Ksp632I       216  GAAGAG               CTCTTC(1/4)
             1078  GAAGAG              
MaeIII         56  GTCAC                ^GT[ACGT]AC
              286  GTGAC               
MamI           16  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              897  GATCCAAATC          
NlaIV         510  GGACCC               GG[ACGT]^[ACGT]CC
             1385  GGGCCC              
PleI          113  GAGTC                GAGTC(4/5)
              279  GAGTC               
SfaNI         861  GCATC                GCATC(5/9)
             1231  GCATC               
SfcI          399  CTATAG               C^T[GA][CT]AG
              992  CTGCAG              
SmlI          811  CTTGAG               C^T[CT][GA]AG
             1317  CTTGAG              
StuI          482  AGGCCT               AGG^CCT
              827  AGGCCT              
StyI          479  CCAAGG               C^C[AT][AT]GG
             1326  CCTTGG              
TfiI          526  GAATC                G^A[AT]TC
              988  GAATC               
Tsp45I         56  GTCAC                ^GT[GC]AC
              286  GTGAC               
XcmI          143  CCAAATACAGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1010  CCACATTTGAGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BfaI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HindIII     HinfI       HphI        ItaI        
MaeI        MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      RsaI        
Sau3AI      Sau96I      ScrFI       Tru9I       TseI        
Tsp509I     TspRI       
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