Restriction map for pF1KB5241
 (Linear) (Six-base) MAPSORT of: KIBB5241.seq  Check: 9752  from: 1  to: 1434



KIBB5241.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AatII G_ACGT'C

Cuts at:      0    1187    1434 
   Size:       1187     247
 
AccI GT'mk_AC

Cuts at:      0    1337    1434 
   Size:       1337      97
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     469     664     694     722    1097    1434 
   Size:        235     234     195      30      28     375     337

  Fragments arranged by size: 

                 375     337     235     234     195      30      28
 
AfeI AGC'GCT

Cuts at:      0     534    1434 
   Size:        534     900
 
AflIII A'CryG_T

Cuts at:      0     737    1434 
   Size:        737     697
 
ApaI G_GGCC'C

Cuts at:      0    1389    1434 
   Size:       1389      45
 
ApoI r'AATT_y

Cuts at:      0     130    1083    1165    1434 
   Size:        130     953      82     269

  Fragments arranged by size: 

                 953     269     130      82
 
BanII G_rGCy'C

Cuts at:      0    1389    1434 
   Size:       1389      45
 
BclI T'GATC_A

Cuts at:      0      18    1434 
   Size:         18    1416
 
BfrBI ATG'CAT

Cuts at:      0    1103    1434 
   Size:       1103     331
 
BglI GCCn_nnn'nGGC

Cuts at:      0     566     933    1434 
   Size:        566     367     501

  Fragments arranged by size: 

                 566     501     367
 
BglII A'GATC_T

Cuts at:      0    1305    1434 
   Size:       1305     129
 
Bme1580I G_kGCm'C

Cuts at:      0    1389    1434 
   Size:       1389      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61    1390    1434 
   Size:         61    1329      44

  Fragments arranged by size: 

                1329      61      44
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1338    1434 
   Size:        832     506      96

  Fragments arranged by size: 

                 832     506      96
 
BsaBI GATnn'nnATC

Cuts at:      0      20     901    1434 
   Size:         20     881     533

  Fragments arranged by size: 

                 881     533      20
 
BsaHI Gr'CG_yC

Cuts at:      0    1184    1434 
   Size:       1184     250
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     291    1434 
   Size:        291    1143
 
BseYI C'CCAG_C

Cuts at:      0     535    1434 
   Size:        535     899
 
Bsp1286I G_dGCh'C

Cuts at:      0    1389    1434 
   Size:       1389      45
 
BsrBI CCG'CTC

Cuts at:      0    1383    1434 
   Size:       1383      51
 
BstEII G'GTnAC_C

Cuts at:      0     285    1434 
   Size:        285    1149
 
BstYI r'GATC_y

Cuts at:      0    1305    1434 
   Size:       1305     129
 
Bsu36I CC'TnA_GG

Cuts at:      0     616    1434 
   Size:        616     818
 
BtsI GCAGTG_nn'

Cuts at:      0    1001    1434 
   Size:       1001     433
 
DraI TTT'AAA

Cuts at:      0     573    1208    1434 
   Size:        573     635     226

  Fragments arranged by size: 

                 635     573     226
 
EarI CTCTTCn'nnn_

Cuts at:      0     211    1073    1434 
   Size:        211     862     361

  Fragments arranged by size: 

                 862     361     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     235     469     664     694     722    1097    1390 
   Size:         61     174     234     195      30      28     375     293

Cuts at:   1390    1434 
   Size:         44

  Fragments arranged by size: 

                 375     293     234     195     174      61      44      30
                  28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     355     619    1434 
   Size:        355     264     815

  Fragments arranged by size: 

                 815     355     264
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1386    1429    1434 
   Size:       1386      43       5

  Fragments arranged by size: 

                1386      43       5
 
EcoRI G'AATT_C

Cuts at:      0     130    1434 
   Size:        130    1304
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     504    1434 
   Size:        504     930
 
HaeII r_GCGC'y

Cuts at:      0     536    1434 
   Size:        536     898
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1413    1434 
   Size:       1413      21
 
HindIII A'AGCT_T

Cuts at:      0     379     676    1314    1434 
   Size:        379     297     638     120

  Fragments arranged by size: 

                 638     379     297     120
 
MfeI C'AATT_G

Cuts at:      0      46    1434 
   Size:         46    1388
 
NsiI A_TGCA'T

Cuts at:      0    1105    1434 
   Size:       1105     329
 
NspI r_CATG'y

Cuts at:      0     741    1434 
   Size:        741     693
 
PciI A'CATG_T

Cuts at:      0     737    1434 
   Size:        737     697
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     646    1434 
   Size:        646     788
 
PpuMI rG'GwC_Cy

Cuts at:      0    1429    1434 
   Size:       1429       5
 
PshAI GACnn'nnGTC

Cuts at:      0     754    1434 
   Size:        754     680
 
PspOMI G'GGCC_C

Cuts at:      0    1385    1434 
   Size:       1385      49
 
PstI C_TGCA'G

Cuts at:      0     996    1434 
   Size:        996     438
 
SapI GCTCTTCn'nnn_

Cuts at:      0     211    1434 
   Size:        211    1223
 
ScaI AGT'ACT

Cuts at:      0    1375    1434 
   Size:       1375      59
 
SfcI C'TryA_G

Cuts at:      0     399     992    1434 
   Size:        399     593     442

  Fragments arranged by size: 

                 593     442     399
 
SmlI C'TyrA_G

Cuts at:      0     811    1317    1434 
   Size:        811     506     117

  Fragments arranged by size: 

                 811     506     117
 
StuI AGG'CCT

Cuts at:      0     484     829    1434 
   Size:        484     345     605

  Fragments arranged by size: 

                 605     484     345
 
StyI C'CwwG_G

Cuts at:      0     479    1326    1434 
   Size:        479     847     108

  Fragments arranged by size: 

                 847     479     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     858    1434 
   Size:        858     576
 
TatI w'GTAC_w

Cuts at:      0       4     713    1373    1434 
   Size:          4     709     660      61

  Fragments arranged by size: 

                 709     660      61       4
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     881     913    1434 
   Size:        881      32     521

  Fragments arranged by size: 

                 881     521      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1427    1434 
   Size:       1427       7
 
XbaI T'CTAG_A

Cuts at:      0    1029    1434 
   Size:       1029     405
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     150    1017    1434 
   Size:        150     867     417

  Fragments arranged by size: 

                 867     417     150
 
ZraI GAC'GTC

Cuts at:      0    1185    1434 
   Size:       1185     249
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI   AflIII     ApaI     ApoI    BanII
     BclI    BfrBI     BglI    BglII Bme1580I     BpmI    BpuEI    BsaBI
    BsaHI    BseRI    BseYI Bsp1286I    BsrBI   BstEII    BstYI   Bsu36I
     BtsI     DraI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I  HindIII     MfeI     NsiI     NspI     PciI    PflMI
    PpuMI    PshAI   PspOMI     PstI     SapI     ScaI     SfcI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRV     FseI
     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XhoI     XmaI     XmnI
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