Restriction map for pF1KB5238
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BbeI        BbsI        BbvCI       BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaI        BsaWI       BseAI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        Eam1105I    Eco47III    Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        MaeI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII         535  GACGTC               GACGT^C
AfaI          144  GTAC                 GT^AC
Alw44I        243  GTGCAC               G^TGCAC
AlwI          294  GATCC                GGATC(4/5)
ApaLI         243  GTGCAC               G^TGCAC
Asp700I       255  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         243  GTGCAC               G[AT]GC[AT]^C
AvaII         436  GGTCC                G^G[AT]CC
BalI          609  TGGCCA               TGG^CCA
BbrPI         400  CACGTG               CAC^GTG
BcgI          530  GCATGGACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          128  CCGGG                CC^[GC]GG
BglI          311  GCCAGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          190  CTGGAG               CTGGAG(16/14)
BsaAI         400  CACGTG               [CT]AC^GT[GA]
BsaBI         591  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          468  CTGCAC               GTGCAG(16/14)
BsiEI         177  CGGCCG               CG[GA][CT]^CG
BsiHKAI       243  GTGCAC               G[AT]GC[AT]^C
BssSI         139  CTCGTG               CACGAG(-5/-1)
DpnII         294  GATC                 ^GATC
DraIII        286  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          177  CGGCCG               C^GGCCG
EarI          114  CTCTTC               CTCTTC(1/4)
EclXI         177  CGGCCG               C^GGCCG
Eco52I        177  CGGCCG               C^GGCCG
EcoNI         281  CCTTCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       422  CCATGG               C^C[AT][AT]GG
HaeII           6  GGCGCT               [GA]GCGC^[CT]
HgaI          157  GACGC                GACGC(5/10)
HinfI         553  GAATC                G^A[ACGT]TC
Ksp632I       114  CTCTTC               CTCTTC(1/4)
KspI           74  CCGCGG               CCGC^GG
MaeIII        515  GTCAC                ^GT[ACGT]AC
MamI          591  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          294  GATC                 ^GATC
MluNI         609  TGGCCA               TGG^CCA
MscI          609  TGGCCA               TGG^CCA
NciI          128  CCGGG                CC^[GC]GG
NcoI          422  CCATGG               C^CATGG
NdeII         294  GATC                 ^GATC
NspI          501  GCATGT               [GA]CATG^[CT]
PmaCI         400  CACGTG               CAC^GTG
PmlI          400  CACGTG               CAC^GTG
PstI           87  CTGCAG               CTGCA^G
PvuII         221  CAGCTG               CAG^CTG
RsaI          144  GTAC                 GT^AC
SacII          74  CCGCGG               CCGC^GG
Sau3AI        294  GATC                 ^GATC
SfcI           87  CTGCAG               C^T[GA][CT]AG
StyI          422  CCATGG               C^C[AT][AT]GG
TfiI          553  GAATC                G^A[AT]TC
Tsp45I        515  GTCAC                ^GT[GC]AC
XmnI          255  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          157  GACGCC               G[GA]^CG[CT]C
              535  GACGTC              
AluI          194  AGCT                 AG^CT
              222  AGCT                
BanII          96  GGGCTC               G[GA]GC[CT]^C
              236  GAGCCC              
BsaHI         157  GACGCC               G[GA]^CG[CT]C
              535  GACGTC              
BsaJI          74  CCGCGG               C^C[ACGT][ACGT]GG
              422  CCATGG              
BseRI         120  CTCCTC               GAGGAG(10/8)
              123  CTCCTC              
BsmFI          46  GTCCC                GGGAC(10/14)
              211  GTCCC               
BsrBI         136  CCGCTC               CCGCTC(-3/-3)
              172  CCGCTC              
BstF5I        581  CATCC                GGATG(2/0)
              492  GGATG               
DdeI          224  CTGAG                C^T[ACGT]AG
              566  CTAAG               
DsaI           74  CCGCGG               C^C[GA][CT]GG
              422  CCATGG              
EaeI          177  CGGCCG               [CT]^GGCC[GA]
              609  TGGCCA              
FokI          581  CATCC                GGATG(9/13)
              492  GGATG               
HapII         101  CCGG                 C^CGG
              128  CCGG                
Hin1I         157  GACGCC               G[GA]^CG[CT]C
              535  GACGTC              
HpaII         101  CCGG                 C^CGG
              128  CCGG                
MspI          101  CCGG                 C^CGG
              128  CCGG                
TspRI         246  CACTG                CA[GC]TG[ACGT][ACGT]^
              392  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AccII       AciI        BbvI        BmyI        BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      EcoRII      
FauI        Fnu4HI      HaeIII      HhaI        HinP1I      
HphI        ItaI        MaeII       MboII       MnlI        
MspA1I      MvaI        MvnI        MwoI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI        
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