Restriction map for pF1KB5192
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfaI        BfrI        BlnI        BsaBI       BsaI        
BsiEI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstUI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          343  GTAGAC               GT^[AC][GT]AC
AccIII       1091  TCCGGA               T^CCGGA
AcsI           22  GAATTT               [GA]^AATT[CT]
AcyI          286  GGCGTC               G[GA]^CG[CT]C
ApoI           22  GAATTT               [GA]^AATT[CT]
AspI          852  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          133  CTCGGG               C^[CT]CG[GA]G
BamHI        1109  GGATCC               G^GATCC
BcgI         1158  GCACCAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII          83  AGATCT               A^GATCT
BpmI          126  CTGGAG               CTGGAG(16/14)
BsaAI        1075  TACGTG               [CT]AC^GT[GA]
BsaHI         286  GGCGTC               G[GA]^CG[CT]C
BsaWI        1091  TCCGGA               [AT]^CCGG[AT]
BseAI        1091  TCCGGA               T^CCGGA
BsgI          208  CTGCAC               GTGCAG(16/14)
BsmBI        1372  CGTCTC               CGTCTC(1/5)
BsmI          957  GAATGC               GAATGC(1/-1)
BsoBI         133  CTCGGG               C^[CT]CG[GA]G
BspEI        1091  TCCGGA               T^CCGGA
BspMI         917  ACCTGC               ACCTGC(4/8)
BsrFI         560  GCCGGT               [GA]^CCGG[CT]
BssSI         250  CTCGTG               CACGAG(-5/-1)
BstXI         620  CCACTTGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        560  GCCGGT               [GA]^CCGG[CT]
EarI          640  CTCTTC               CTCTTC(1/4)
EcoRV         510  GATATC               GAT^ATC
HgaI          287  GCGTC                GACGC(5/10)
Hin1I         286  GGCGTC               G[GA]^CG[CT]C
Ksp632I       640  CTCTTC               CTCTTC(1/4)
MroI         1091  TCCGGA               T^CCGGA
MseI         1192  TTAA                 T^TAA
NcoI          710  CCATGG               C^CATGG
PflFI         852  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1334  CCAGTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          568  CTGCAG               CTGCA^G
SfcI          568  CTGCAG               C^T[GA][CT]AG
SphI          156  GCATGC               GCATG^C
Tru9I        1192  TTAA                 T^TAA
Tsp509I        23  AATT                 ^AATT
Tth111I       852  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1334  CCAGTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI          440  GTAC                 GT^AC
              648  GTAC                
ApaI          502  GGGCCC               GGGCC^C
              630  GGGCCC              
AspHI        1208  GAGCTC               G[AT]GC[AT]^C
             1321  GAGCTC              
BbsI          289  GTCTTC               GAAGAC(2/6)
              496  GTCTTC              
BcnI          426  CCGGG                CC^[GC]GG
              581  CCCGG               
BglI          391  GCCATGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              796  GCCACGTTGGC         
BlpI          231  GCTCAGC              GC^T[ACGT]AGC
             1323  GCTCAGC             
Bpu1102I      231  GCTCAGC              GC^T[ACGT]AGC
             1323  GCTCAGC             
BpuAI         289  GTCTTC               GAAGAC(2/6)
              496  GTCTTC              
BsiHKAI      1208  GAGCTC               G[AT]GC[AT]^C
             1321  GAGCTC              
BstAPI         33  GCAGCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              360  GCACAAGATGC         
BstEII        667  GGTGACC              G^GT[ACGT]ACC
              993  GGTCACC             
BstPI         667  GGTGACC              G^GT[ACGT]ACC
              993  GGTCACC             
CelII         231  GCTCAGC              GC^T[ACGT]AGC
             1323  GCTCAGC             
CfoI         1010  GCGC                 GCG^C
             1098  GCGC                
EcoO65I       667  GGTGACC              G^GT[ACGT]ACC
              993  GGTCACC             
EcoT14I       540  CCAAGG               C^C[AT][AT]GG
              710  CCATGG              
HaeII        1009  AGCGCC               [GA]GCGC^[CT]
             1097  AGCGCC              
HhaI         1010  GCGC                 GCG^C
             1098  GCGC                
HinP1I       1010  GCGC                 G^CGC
             1098  GCGC                
MaeII         799  ACGT                 A^CGT
             1076  ACGT                
MslI          621  CACTTGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1071  CACTTACGTG          
MspA1I        205  CAGCTG               C[AC]G^C[GT]G
             1326  CAGCTG              
NciI          426  CCGGG                CC^[GC]GG
              581  CCCGG               
NspI          156  GCATGC               [GA]CATG^[CT]
             1083  GCATGT              
PpuMI         845  GGGACCT              [GA]G^G[AT]CC[CT]
              973  GGGACCT             
PvuII         205  CAGCTG               CAG^CTG
             1326  CAGCTG              
RsaI          440  GTAC                 GT^AC
              648  GTAC                
SacI         1208  GAGCTC               GAGCT^C
             1321  GAGCTC              
StyI          540  CCAAGG               C^C[AT][AT]GG
              710  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BalI        BanI        BanII       BbvI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        XhoII       
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