Restriction map for pF1KB5192
 (Linear) (Six-base) MAPSORT of: KIBB5192.seq  Check: 2457  from: 1  to: 1383



KIBB5192.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     344    1383 
   Size:        344    1039
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     172     741    1011    1383 
   Size:        168       4     569     270     372

  Fragments arranged by size: 

                 569     372     270     168       4
 
AleI CACnn'nnGTG

Cuts at:      0     625    1075    1383 
   Size:        625     450     308

  Fragments arranged by size: 

                 625     450     308
 
AlwNI CAG_nnn'CTG

Cuts at:      0      39    1121    1340    1383 
   Size:         39    1082     219      43

  Fragments arranged by size: 

                1082     219      43      39
 
ApaI G_GGCC'C

Cuts at:      0     506     634    1383 
   Size:        506     128     749

  Fragments arranged by size: 

                 749     506     128
 
ApoI r'AATT_y

Cuts at:      0      22    1383 
   Size:         22    1361
 
AvaI C'yCGr_G

Cuts at:      0     133    1383 
   Size:        133    1250
 
BamHI G'GATC_C

Cuts at:      0    1109    1383 
   Size:       1109     274
 
BanI G'GyrC_C

Cuts at:      0     444     487     706    1139    1383 
   Size:        444      43     219     433     244

  Fragments arranged by size: 

                 444     433     244     219      43
 
BanII G_rGCy'C

Cuts at:      0     506     634    1212    1243    1325    1383 
   Size:        506     128     578      31      82      58

  Fragments arranged by size: 

                 578     506     128      82      58      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     282     489    1383 
   Size:        282     207     894

  Fragments arranged by size: 

                 894     282     207
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1147    1181    1383 
   Size:       1147      34     202

  Fragments arranged by size: 

                1147     202      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     397     802    1383 
   Size:        397     405     581

  Fragments arranged by size: 

                 581     405     397
 
BglII A'GATC_T

Cuts at:      0      83    1383 
   Size:         83    1300
 
BlpI GC'TnA_GC

Cuts at:      0     232    1324    1383 
   Size:        232    1092      59

  Fragments arranged by size: 

                1092     232      59
 
Bme1580I G_kGCm'C

Cuts at:      0     378     449     490     506     634     709    1144    1383 
   Size:        378      71      41      16     128      75     435     239

  Fragments arranged by size: 

                 435     378     239     128      75      71      41      16
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1350    1383 
   Size:       1350      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     147    1383 
   Size:        147    1236
 
BsaAI yAC'GTr

Cuts at:      0    1077    1383 
   Size:       1077     306
 
BsaHI Gr'CG_yC

Cuts at:      0     287    1383 
   Size:        287    1096
 
BsaWI w'CCGG_w

Cuts at:      0    1091    1383 
   Size:       1091     292
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1225    1255    1383 
   Size:       1225      30     128

  Fragments arranged by size: 

                1225     128      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     838    1291    1383 
   Size:        112     726     453      92

  Fragments arranged by size: 

                 726     453     112      92
 
BseYI C'CCAG_C

Cuts at:      0      65    1328    1383 
   Size:         65    1263      55

  Fragments arranged by size: 

                1263      65      55
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193    1383 
   Size:        193    1190
 
BsiHKAI G_wGCw'C

Cuts at:      0    1212    1325    1383 
   Size:       1212     113      58

  Fragments arranged by size: 

                1212     113      58
 
BsmI GAATG_Cn'

Cuts at:      0     963    1383 
   Size:        963     420
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1378    1383 
   Size:       1378       5
 
Bsp1286I G_dGCh'C

Cuts at:      0     378     449     490     506     634     709    1144    1212 
   Size:        378      71      41      16     128      75     435      68

Cuts at:   1212    1243    1325    1383 
   Size:         31      82      58

  Fragments arranged by size: 

                 435     378     128      82      75      71      68      58
                  41      31      16
 
BspEI T'CCGG_A

Cuts at:      0    1091    1383 
   Size:       1091     292
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     926    1383 
   Size:        926     457
 
BsrFI r'CCGG_y

Cuts at:      0     560    1383 
   Size:        560     823
 
BssSI C'ACGA_G

Cuts at:      0     250    1383 
   Size:        250    1133
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      39     366    1383 
   Size:         39     327    1017

  Fragments arranged by size: 

                1017     327      39
 
BstEII G'GTnAC_C

Cuts at:      0     667     993    1383 
   Size:        667     326     390

  Fragments arranged by size: 

                 667     390     326
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     627    1383 
   Size:        627     756
 
BstYI r'GATC_y

Cuts at:      0      83    1109    1361    1383 
   Size:         83    1026     252      22

  Fragments arranged by size: 

                1026     252      83      22
 
BtgI C'CryG_G

Cuts at:      0     167     710     791    1383 
   Size:        167     543      81     592

  Fragments arranged by size: 

                 592     543     167      81
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1150    1383 
   Size:       1150     233
 
BtsI GCAGTG_nn'

Cuts at:      0     407    1383 
   Size:        407     976
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     149     184    1383 
   Size:        149      35    1199

  Fragments arranged by size: 

                1199     149      35
 
EaeI y'GGCC_r

Cuts at:      0       2     389     794    1214    1383 
   Size:          2     387     405     420     169

  Fragments arranged by size: 

                 420     405     387     169       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     646    1383 
   Size:        646     737
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     955    1383 
   Size:        955     428
 
EcoICRI GAG'CTC

Cuts at:      0    1210    1323    1383 
   Size:       1210     113      60

  Fragments arranged by size: 

                1210     113      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     147     168     172     741    1011    1383 
   Size:        147      21       4     569     270     372

  Fragments arranged by size: 

                 569     372     270     147      21       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     503     631     846     974    1383 
   Size:         72     431     128     215     128     409

  Fragments arranged by size: 

                 431     409     215     128     128      72
 
EcoRV GAT'ATC

Cuts at:      0     512    1383 
   Size:        512     871
 
HaeII r_GCGC'y

Cuts at:      0    1013    1101    1383 
   Size:       1013      88     282

  Fragments arranged by size: 

                1013     282      88
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1346    1378    1383 
   Size:       1346      32       5

  Fragments arranged by size: 

                1346      32       5
 
MscI TGG'CCA

Cuts at:      0       4     391    1216    1383 
   Size:          4     387     825     167

  Fragments arranged by size: 

                 825     387     167       4
 
MslI CAynn'nnrTG

Cuts at:      0     625    1075    1383 
   Size:        625     450     308

  Fragments arranged by size: 

                 625     450     308
 
MspA1I CmG'CkG

Cuts at:      0     207    1328    1383 
   Size:        207    1121      55

  Fragments arranged by size: 

                1121     207      55
 
NcoI C'CATG_G

Cuts at:      0     710    1383 
   Size:        710     673
 
NspI r_CATG'y

Cuts at:      0     160    1087    1383 
   Size:        160     927     296

  Fragments arranged by size: 

                 927     296     160
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1340    1383 
   Size:       1340      43
 
PpuMI rG'GwC_Cy

Cuts at:      0     846     974    1383 
   Size:        846     128     409

  Fragments arranged by size: 

                 846     409     128
 
PspOMI G'GGCC_C

Cuts at:      0     502     630    1383 
   Size:        502     128     753

  Fragments arranged by size: 

                 753     502     128
 
PstI C_TGCA'G

Cuts at:      0     572    1383 
   Size:        572     811
 
PvuII CAG'CTG

Cuts at:      0     207    1328    1383 
   Size:        207    1121      55

  Fragments arranged by size: 

                1121     207      55
 
SacI G_AGCT'C

Cuts at:      0    1212    1325    1383 
   Size:       1212     113      58

  Fragments arranged by size: 

                1212     113      58
 
SfcI C'TryA_G

Cuts at:      0     568    1383 
   Size:        568     815
 
SphI G_CATG'C

Cuts at:      0     160    1383 
   Size:        160    1223
 
StyI C'CwwG_G

Cuts at:      0     540     710    1383 
   Size:        540     170     673

  Fragments arranged by size: 

                 673     540     170
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316     875    1383 
   Size:        316     559     508

  Fragments arranged by size: 

                 559     508     316
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1101    1133    1383 
   Size:       1101      32     250

  Fragments arranged by size: 

                1101     250      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     855    1383 
   Size:        855     528
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     708     798    1224    1383 
   Size:        708      90     426     159

  Fragments arranged by size: 

                 708     426     159      90
 

 Enzymes that do cut:

     AccI     AcuI     AleI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI     BcgI     BglI    BglII     BlpI Bme1580I
     BmrI     BpmI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV
    HaeII    Hin4I     MscI     MslI   MspA1I     NcoI     NspI    PflMI
    PpuMI   PspOMI     PstI    PvuII     SacI     SfcI     SphI     StyI
    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI    BciVI     BclI    BfrBI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsiEI    BsiWI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I     ClaI
     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TatI     XbaI     XhoI     XmaI     XmnI     ZraI
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