Restriction map for pF1KB5180
 (Linear) (Six-base) MAPSORT of: KIBB5180.seq  Check: 3112  from: 1  to: 390



KIBB5180.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     390 
   Size:         37     353
 
ApoI r'AATT_y

Cuts at:      0     116     390 
   Size:        116     274
 
AvaI C'yCGr_G

Cuts at:      0      88     390 
   Size:         88     302
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     390 
   Size:        291      99
 
BsaWI w'CCGG_w

Cuts at:      0     153     390 
   Size:        153     237
 
BspHI T'CATG_A

Cuts at:      0      35     390 
   Size:         35     355
 
BsrGI T'GTAC_A

Cuts at:      0     140     390 
   Size:        140     250
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     390 
   Size:         37     353
 
MslI CAynn'nnrTG

Cuts at:      0     191     390 
   Size:        191     199
 
SmaI CCC'GGG

Cuts at:      0      90     390 
   Size:         90     300
 
SmlI C'TyrA_G

Cuts at:      0     306     390 
   Size:        306      84
 
TatI w'GTAC_w

Cuts at:      0     140     390 
   Size:        140     250
 
XmaI C'CCGG_G

Cuts at:      0      88     390 
   Size:         88     302
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BpuEI    BsaWI    BspHI    BsrGI  Eco57MI
     MslI     SmaI     SmlI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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