Restriction map for pF1KB5178
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FseI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SspI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI         1323  GTCTAC               GT^[AC][GT]AC
AccII        1429  CGCG                 CG^CG
Asp700I       864  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         528  TGCGCA               TGC^GCA
BclI         1395  TGATCA               T^GATCA
BglI          547  GCCCTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          963  CTGGAG               CTGGAG(16/14)
BsaI         1072  GGTCTC               GGTCTC(1/5)
BsrFI          37  GCCGGT               [GA]^CCGG[CT]
BstAPI       1176  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1429  CGCG                 CG^CG
BstYI        1162  GGATCT               [GA]^GATC[CT]
Cfr10I         37  GCCGGT               [GA]^CCGG[CT]
EcoNI        1056  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1395  TGATCA               T^GATCA
FspI          528  TGCGCA               TGC^GCA
MaeII         842  ACGT                 A^CGT
MflI         1162  GGATCT               [GA]^GATC[CT]
MseI         1337  TTAA                 T^TAA
MvnI         1429  CGCG                 CG^CG
NcoI          384  CCATGG               C^CATGG
NspI          356  GCATGC               [GA]CATG^[CT]
SmlI          995  CTCAAG               C^T[CT][GA]AG
SphI          356  GCATGC               GCATG^C
StuI          645  AGGCCT               AGG^CCT
Tru9I        1337  TTAA                 T^TAA
XcmI         1139  CCATGCCCCAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1162  GGATCT               [GA]^GATC[CT]
XmnI          864  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AlwI         1032  GATCC                GGATC(4/5)
             1162  GGATC               
AocI           48  CCTGAGG              CC^T[ACGT]AGG
             1345  CCTGAGG             
AspHI          11  GAGCAC               G[AT]GC[AT]^C
             1446  GAGCAC              
BalI          326  TGGCCA               TGG^CCA
             1433  TGGCCA              
BanI          421  GGCACC               G^G[CT][GA]CC
              949  GGCACC              
BanII         886  GAGCCC               G[GA]GC[CT]^C
             1312  GAGCCC              
BbsI          292  GAAGAC               GAAGAC(2/6)
              783  GAAGAC              
BfaI          268  CTAG                 C^TAG
              571  CTAG                
BlpI         1195  GCTGAGC              GC^T[ACGT]AGC
             1443  GCTGAGC             
Bpu1102I     1195  GCTGAGC              GC^T[ACGT]AGC
             1443  GCTGAGC             
BpuAI         292  GAAGAC               GAAGAC(2/6)
              783  GAAGAC              
BsiHKAI        11  GAGCAC               G[AT]GC[AT]^C
             1446  GAGCAC              
BsmAI        1073  GTCTC                GTCTC(1/5)
             1116  GTCTC               
Bsp1407I      107  TGTACA               T^GTACA
              558  TGTACA              
BsrDI         310  GCAATG               GCAATG(2/0)
             1244  GCAATG              
BsrGI         107  TGTACA               T^GTACA
              558  TGTACA              
BstEII         97  GGTGACC              G^GT[ACGT]ACC
             1359  GGTGACC             
BstPI          97  GGTGACC              G^GT[ACGT]ACC
             1359  GGTGACC             
BstXI         329  CCAACTTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              617  CCACGGTGGTGG        
Bsu36I         48  CCTGAGG              CC^T[ACGT]AGG
             1345  CCTGAGG             
CelII        1195  GCTGAGC              GC^T[ACGT]AGC
             1443  GCTGAGC             
DsaI          384  CCATGG               C^C[GA][CT]GG
              617  CCACGG              
Eco81I         48  CCTGAGG              CC^T[ACGT]AGG
             1345  CCTGAGG             
EcoO65I        97  GGTGACC              G^GT[ACGT]ACC
             1359  GGTGACC             
FauI         1204  CCCGC                CCCGC(4/6)
             1440  CCCGC               
HaeII        1015  AGCGCC               [GA]GCGC^[CT]
             1402  AGCGCC              
HinfI         993  GACTC                G^A[ACGT]TC
             1321  GAGTC               
MaeI          268  CTAG                 C^TAG
              571  CTAG                
MluNI         326  TGGCCA               TGG^CCA
             1433  TGGCCA              
MscI          326  TGGCCA               TGG^CCA
             1433  TGGCCA              
PleI          993  GACTC                GAGTC(4/5)
             1321  GAGTC               
PpuMI         536  AGGTCCT              [GA]G^G[AT]CC[CT]
             1178  AGGACCT             
SbfI          549  CCTGCAGG             CCTGCA^GG
             1173  CCTGCAGG            
Sse8387I      549  CCTGCAGG             CCTGCA^GG
             1173  CCTGCAGG            
SspBI         107  TGTACA               T^GTACA
              558  TGTACA              
Tsp509I       213  AATT                 ^AATT
             1238  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BbvI        BcnI        BmyI        BsaJI       BsgI        
BsiYI       BslI        Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
Tsp45I      TspRI       
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