Restriction map for pF1KB5178
 (Linear) (Six-base) MAPSORT of: KIBB5178.seq  Check: 2867  from: 1  to: 1455



KIBB5178.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      85    1455 
   Size:         85    1370
 
AccI GT'mk_AC

Cuts at:      0    1324    1455 
   Size:       1324     131
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1455 
   Size:        676     779
 
AleI CACnn'nnGTG

Cuts at:      0     622     919     965    1455 
   Size:        622     297      46     490

  Fragments arranged by size: 

                 622     490     297      46
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     499     531    1455 
   Size:        499      32     924

  Fragments arranged by size: 

                 924     499      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     567     609     678     925    1182    1455 
   Size:        567      42      69     247     257     273

  Fragments arranged by size: 

                 567     273     257     247      69      42
 
BanI G'GyrC_C

Cuts at:      0     421     949    1455 
   Size:        421     528     506

  Fragments arranged by size: 

                 528     506     421
 
BanII G_rGCy'C

Cuts at:      0     890    1316    1455 
   Size:        890     426     139

  Fragments arranged by size: 

                 890     426     139
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299     790    1455 
   Size:        299     491     665

  Fragments arranged by size: 

                 665     491     299
 
BclI T'GATC_A

Cuts at:      0    1395    1455 
   Size:       1395      60
 
BglI GCCn_nnn'nGGC

Cuts at:      0     553    1455 
   Size:        553     902
 
BlpI GC'TnA_GC

Cuts at:      0    1196    1444    1455 
   Size:       1196     248      11

  Fragments arranged by size: 

                1196     248      11
 
Bme1580I G_kGCm'C

Cuts at:      0     952    1004    1455 
   Size:        952      52     451

  Fragments arranged by size: 

                 952     451      52
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      12    1455 
   Size:         12    1443
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     984    1455 
   Size:        984     471
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     980    1455 
   Size:        980     475
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1078    1455 
   Size:       1078     377
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     499     529     797     827    1455 
   Size:        499      30     268      30     628

  Fragments arranged by size: 

                 628     499     268      30      30
 
BseYI C'CCAG_C

Cuts at:      0     602    1455 
   Size:        602     853
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     495     634     691    1198    1399    1455 
   Size:        495     139      57     507     201      56

  Fragments arranged by size: 

                 507     495     201     139      57      56
 
BsiHKAI G_wGCw'C

Cuts at:      0      15    1450    1455 
   Size:         15    1435       5

  Fragments arranged by size: 

                1435      15       5
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     890     952    1004    1316    1450    1455 
   Size:         15     875      62      52     312     134       5

  Fragments arranged by size: 

                 875     312     134      62      52      15       5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      85     213    1181    1190    1238    1455 
   Size:         85     128     968       9      48     217

  Fragments arranged by size: 

                 968     217     128      85      48       9
 
BsrDI GCAATG_nn'

Cuts at:      0     317    1251    1455 
   Size:        317     934     204

  Fragments arranged by size: 

                 934     317     204
 
BsrFI r'CCGG_y

Cuts at:      0      37    1455 
   Size:         37    1418
 
BsrGI T'GTAC_A

Cuts at:      0     107     558    1455 
   Size:        107     451     897

  Fragments arranged by size: 

                 897     451     107
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1182    1455 
   Size:       1182     273
 
BstEII G'GTnAC_C

Cuts at:      0      97    1359    1455 
   Size:         97    1262      96

  Fragments arranged by size: 

                1262      97      96
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     624    1455 
   Size:        336     288     831

  Fragments arranged by size: 

                 831     336     288
 
BstYI r'GATC_y

Cuts at:      0    1162    1455 
   Size:       1162     293
 
Bsu36I CC'TnA_GG

Cuts at:      0      49    1346    1455 
   Size:         49    1297     109

  Fragments arranged by size: 

                1297     109      49
 
BtgI C'CryG_G

Cuts at:      0     384     617    1455 
   Size:        384     233     838

  Fragments arranged by size: 

                 838     384     233
 
BtsI GCAGTG_nn'

Cuts at:      0    1406    1455 
   Size:       1406      49
 
EaeI y'GGCC_r

Cuts at:      0     326     881    1433    1455 
   Size:        326     555     552      22

  Fragments arranged by size: 

                 555     552     326      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676     984    1455 
   Size:        676     308     471

  Fragments arranged by size: 

                 676     471     308
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1060    1455 
   Size:       1060     395
 
EcoO109I rG'GnC_Cy

Cuts at:      0     246     537     564     675    1179    1455 
   Size:        246     291      27     111     504     276

  Fragments arranged by size: 

                 504     291     276     246     111      27
 
FspI TGC'GCA

Cuts at:      0     530    1455 
   Size:        530     925
 
FspAI rTGC'GCAy

Cuts at:      0     530    1455 
   Size:        530     925
 
HaeII r_GCGC'y

Cuts at:      0    1019    1406    1455 
   Size:       1019     387      49

  Fragments arranged by size: 

                1019     387      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     970    1002    1023    1055    1455 
   Size:        970      32      21      32     400

  Fragments arranged by size: 

                 970     400      32      32      21
 
MscI TGG'CCA

Cuts at:      0     328    1435    1455 
   Size:        328    1107      20

  Fragments arranged by size: 

                1107     328      20
 
MslI CAynn'nnrTG

Cuts at:      0     622     919     965    1258    1455 
   Size:        622     297      46     293     197

  Fragments arranged by size: 

                 622     297     293     197      46
 
MspA1I CmG'CkG

Cuts at:      0     159     261     463     606    1443    1455 
   Size:        159     102     202     143     837      12

  Fragments arranged by size: 

                 837     202     159     143     102      12
 
NcoI C'CATG_G

Cuts at:      0     384    1455 
   Size:        384    1071
 
NspI r_CATG'y

Cuts at:      0     360    1455 
   Size:        360    1095
 
PasI CC'CwG_GG

Cuts at:      0     579    1455 
   Size:        579     876
 
PfoI T'CCnGG_A

Cuts at:      0     974    1455 
   Size:        974     481
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     499     531    1455 
   Size:        499      32     924

  Fragments arranged by size: 

                 924     499      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     537    1179    1455 
   Size:        537     642     276

  Fragments arranged by size: 

                 642     537     276
 
PstI C_TGCA'G

Cuts at:      0      96     554    1178    1455 
   Size:         96     458     624     277

  Fragments arranged by size: 

                 624     458     277      96
 
PvuII CAG'CTG

Cuts at:      0     159     261     463     606    1455 
   Size:        159     102     202     143     849

  Fragments arranged by size: 

                 849     202     159     143     102
 
SbfI CC_TGCA'GG

Cuts at:      0     554    1178    1455 
   Size:        554     624     277

  Fragments arranged by size: 

                 624     554     277
 
SfcI C'TryA_G

Cuts at:      0      92     250     472     550    1174    1455 
   Size:         92     158     222      78     624     281

  Fragments arranged by size: 

                 624     281     222     158      92      78
 
SmlI C'TyrA_G

Cuts at:      0     995    1455 
   Size:        995     460
 
SphI G_CATG'C

Cuts at:      0     360    1455 
   Size:        360    1095
 
StuI AGG'CCT

Cuts at:      0     647    1455 
   Size:        647     808
 
StyI C'CwwG_G

Cuts at:      0     384     449     493     852    1455 
   Size:        384      65      44     359     603

  Fragments arranged by size: 

                 603     384     359      65      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     475     891    1455 
   Size:        475     416     564

  Fragments arranged by size: 

                 564     475     416
 
TatI w'GTAC_w

Cuts at:      0     107     558    1455 
   Size:        107     451     897

  Fragments arranged by size: 

                 897     451     107
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     797     829    1455 
   Size:        797      32     626

  Fragments arranged by size: 

                 797     626      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1146    1455 
   Size:       1146     309
 
XmnI GAAnn'nnTTC

Cuts at:      0     868    1455 
   Size:        868     587
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AloI    AlwNI     BanI    BanII
     BbsI     BclI     BglI     BlpI Bme1580I     BmrI     BpmI    BpuEI
     BsaI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI     EaeI  Eco57MI    EcoNI EcoO109I     FspI    FspAI    HaeII
    Hin4I     MscI     MslI   MspA1I     NcoI     NspI     PasI     PfoI
     PpiI    PpuMI     PstI    PvuII     SbfI     SfcI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI
    BglII    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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