Restriction map for pF1KB5167
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbrPI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        MaeI        
MamI        MluI        MroI        MslI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AlwNI         922  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          679  GGGCCC               GGGCC^C
BbeI         1504  GGCGCC               GGCGC^C
BbvCI        1377  CCTCAGC              CCTCAGC(-5/-2)
BcgI          618  CGACGTGTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1232  CCCGG                CC^[GC]GG
BpmI         1462  CTGGAG               CTGGAG(16/14)
BsmBI        1468  GAGACG               CGTCTC(1/5)
BspLU11I      800  ACATGT               A^CATGT
BsrBI        1545  GAGCGG               CCGCTC(-3/-3)
BsrI          338  CCAGT                ACTGG(1/-1)
BstAPI       1320  GCATGGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         459  GGATCT               [GA]^GATC[CT]
DrdI         1363  GACCGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           50  CGGCCG               C^GGCCG
EclXI          50  CGGCCG               C^GGCCG
Eco52I         50  CGGCCG               C^GGCCG
HgaI         1006  GACGC                GACGC(5/10)
KasI         1504  GGCGCC               G^GCGCC
KspI           33  CCGCGG               CCGC^GG
MfeI          562  CAATTG               C^AATTG
MflI          459  GGATCT               [GA]^GATC[CT]
MunI          562  CAATTG               C^AATTG
NarI         1504  GGCGCC               GG^CGCC
NciI         1232  CCCGG                CC^[GC]GG
NcoI          905  CCATGG               C^CATGG
PshAI        1046  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          286  GAGCTC               GAGCT^C
SacII          33  CCGCGG               CCGC^GG
SalI         1519  GTCGAC               G^TCGAC
SfoI         1504  GGCGCC               GGC^GCC
SspI          251  AATATT               AAT^ATT
StuI         1424  AGGCCT               AGG^CCT
TfiI          915  GAATC                G^A[AT]TC
Tsp509I       563  AATT                 ^AATT
XcmI         1298  CCAACGCCATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         459  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          295  GTCTAC               GT^[AC][GT]AC
             1519  GTCGAC              
AcyI         1006  GACGCC               G[GA]^CG[CT]C
             1504  GGCGCC              
AfaI          593  GTAC                 GT^AC
              996  GTAC                
AflIII        620  ACGTGT               A^C[GA][CT]GT
              800  ACATGT              
AlwI          567  GATCC                GGATC(4/5)
              459  GGATC               
Aor51HI        80  AGCGCT               AGC^GCT
              111  AGCGCT              
BalI          479  TGGCCA               TGG^CCA
              902  TGGCCA              
BglI          968  GCCTGTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1303  GCCATGCTGGC         
BsaHI        1006  GACGCC               G[GA]^CG[CT]C
             1504  GGCGCC              
BsiEI          50  CGGCCG               CG[GA][CT]^CG
             1362  CGACCG              
BsmAI         558  GAGAC                GTCTC(1/5)
             1468  GAGAC               
BstEII       1185  GGTCACC              G^GT[ACGT]ACC
             1371  GGTCACC             
BstPI        1185  GGTCACC              G^GT[ACGT]ACC
             1371  GGTCACC             
Eco47III       80  AGCGCT               AGC^GCT
              111  AGCGCT              
EcoNI         182  CCTCACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1254  CCTTGAGGAGG         
EcoO65I      1185  GGTCACC              G^GT[ACGT]ACC
             1371  GGTCACC             
Hin1I        1006  GACGCC               G[GA]^CG[CT]C
             1504  GGCGCC              
HincII        328  GTTGAC               GT[CT]^[GA]AC
             1519  GTCGAC              
HindII        328  GTTGAC               GT[CT]^[GA]AC
             1519  GTCGAC              
MluNI         479  TGGCCA               TGG^CCA
              902  TGGCCA              
MscI          479  TGGCCA               TGG^CCA
              902  TGGCCA              
NspI          800  ACATGT               [GA]CATG^[CT]
             1346  GCATGT              
PpuMI         467  GGGTCCT              [GA]G^G[AT]CC[CT]
             1044  GGGACCC             
RsaI          593  GTAC                 GT^AC
              996  GTAC                
SfaNI         139  GATGC                GCATC(5/9)
              728  GCATC               
SmlI          383  CTCAAG               C^T[CT][GA]AG
             1255  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccII       AciI        AluI        AspHI       AvaII       
BanI        BanII       BbsI        BbvI        BmyI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BsrFI       BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PleI        PvuII       
Sau3AI      Sau96I      ScrFI       StyI        TaqI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
Van91I      
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