Restriction map for pF1KB5167
 (Linear) (Six-base) MAPSORT of: KIBB5167.seq  Check: 7046  from: 1  to: 1560



KIBB5167.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1188    1560 
   Size:       1188     372
 
AccI GT'mk_AC

Cuts at:      0     296    1520    1560 
   Size:        296    1224      40

  Fragments arranged by size: 

                1224     296      40
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1060    1560 
   Size:        832     228     500

  Fragments arranged by size: 

                 832     500     228
 
AfeI AGC'GCT

Cuts at:      0      82     113    1560 
   Size:         82      31    1447

  Fragments arranged by size: 

                1447      82      31
 
AflIII A'CryG_T

Cuts at:      0     620     800    1560 
   Size:        620     180     760

  Fragments arranged by size: 

                 760     620     180
 
AlwNI CAG_nnn'CTG

Cuts at:      0     927    1560 
   Size:        927     633
 
ApaI G_GGCC'C

Cuts at:      0     683    1560 
   Size:        683     877
 
BanI G'GyrC_C

Cuts at:      0    1129    1177    1504    1560 
   Size:       1129      48     327      56

  Fragments arranged by size: 

                1129     327      56      48
 
BanII G_rGCy'C

Cuts at:      0     290     683    1094    1560 
   Size:        290     393     411     466

  Fragments arranged by size: 

                 466     411     393     290
 
BbeI G_GCGC'C

Cuts at:      0    1508    1560 
   Size:       1508      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      52     107     839    1253    1535    1560 
   Size:         52      55     732     414     282      25

  Fragments arranged by size: 

                 732     414     282      55      52      25
 
BbvCI CC'TCA_GC

Cuts at:      0    1378    1560 
   Size:       1378     182
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     607     641    1560 
   Size:        607      34     919

  Fragments arranged by size: 

                 919     607      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     974    1309    1560 
   Size:        974     335     251

  Fragments arranged by size: 

                 974     335     251
 
Bme1580I G_kGCm'C

Cuts at:      0     175     683     787    1560 
   Size:        175     508     104     773

  Fragments arranged by size: 

                 773     508     175     104
 
BmgBI CAC'GTC

Cuts at:      0     621     774    1560 
   Size:        621     153     786

  Fragments arranged by size: 

                 786     621     153
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     332    1560 
   Size:        332    1228
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     441    1491    1560 
   Size:        441    1050      69

  Fragments arranged by size: 

                1050     441      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1483    1560 
   Size:       1483      77
 
Bpu10I CC'TnA_GC

Cuts at:      0    1378    1560 
   Size:       1378     182
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     368    1276    1560 
   Size:        368     908     284

  Fragments arranged by size: 

                 908     368     284
 
BsaHI Gr'CG_yC

Cuts at:      0    1007    1505    1560 
   Size:       1007     498      55

  Fragments arranged by size: 

                1007     498      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     955     985    1560 
   Size:        955      30     575

  Fragments arranged by size: 

                 955     575      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     973     976    1102    1273    1480    1507    1555 
   Size:        109     864       3     126     171     207      27      48

Cuts at:   1555    1560 
   Size:          5

  Fragments arranged by size: 

                 864     207     171     126     109      48      27       5
                   3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1165    1372    1387    1560 
   Size:       1165     207      15     173

  Fragments arranged by size: 

                1165     207     173      15
 
BsiEI CG_ry'CG

Cuts at:      0      53    1365    1560 
   Size:         53    1312     195

  Fragments arranged by size: 

                1312     195      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     290     363    1205    1331    1560 
   Size:        290      73     842     126     229

  Fragments arranged by size: 

                 842     290     229     126      73
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1462    1560 
   Size:       1462      98
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     290     363     683     787    1094    1205    1331 
   Size:        175     115      73     320     104     307     111     126

Cuts at:   1331    1560 
   Size:        229

  Fragments arranged by size: 

                 320     307     229     175     126     115     111     104
                  73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1188    1394    1409    1560 
   Size:       1188     206      15     151

  Fragments arranged by size: 

                1188     206     151      15
 
BsrBI CCG'CTC

Cuts at:      0    1547    1560 
   Size:       1547      13
 
BsrFI r'CCGG_y

Cuts at:      0    1110    1264    1357    1560 
   Size:       1110     154      93     203

  Fragments arranged by size: 

                1110     203     154      93
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1326    1560 
   Size:       1326     234
 
BstEII G'GTnAC_C

Cuts at:      0    1185    1371    1560 
   Size:       1185     186     189

  Fragments arranged by size: 

                1185     189     186
 
BstYI r'GATC_y

Cuts at:      0     459    1560 
   Size:        459    1101
 
BtgI C'CryG_G

Cuts at:      0      33     883     899     905    1181    1560 
   Size:         33     850      16       6     276     379

  Fragments arranged by size: 

                 850     379     276      33      16       6
 
BtsI GCAGTG_nn'

Cuts at:      0    1389    1560 
   Size:       1389     171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1369    1560 
   Size:       1369     191
 
EaeI y'GGCC_r

Cuts at:      0      50     261     479     902    1166    1560 
   Size:         50     211     218     423     264     394

  Fragments arranged by size: 

                 423     394     264     218     211      50
 
EagI C'GGCC_G

Cuts at:      0      50    1560 
   Size:         50    1510
 
EarI CTCTTCn'nnn_

Cuts at:      0     550     822     950    1475    1560 
   Size:        550     272     128     525      85

  Fragments arranged by size: 

                 550     525     272     128      85
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     407    1560 
   Size:        407    1153
 
EcoICRI GAG'CTC

Cuts at:      0     288    1560 
   Size:        288    1272
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1060    1483    1560 
   Size:        832     228     423      77

  Fragments arranged by size: 

                 832     423     228      77
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     186    1258    1560 
   Size:        186    1072     302

  Fragments arranged by size: 

                1072     302     186
 
EcoO109I rG'GnC_Cy

Cuts at:      0     468     679    1045    1138    1560 
   Size:        468     211     366      93     422

  Fragments arranged by size: 

                 468     422     366     211      93
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1270    1560 
   Size:       1270     290
 
HaeII r_GCGC'y

Cuts at:      0      84     115     357    1508    1560 
   Size:         84      31     242    1151      52

  Fragments arranged by size: 

                1151     242      84      52      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     522     551     554     583     955     987    1560 
   Size:        522      29       3      29     372      32     573

  Fragments arranged by size: 

                 573     522     372      32      29      29       3
 
HincII GTy'rAC

Cuts at:      0     330    1521    1560 
   Size:        330    1191      39

  Fragments arranged by size: 

                1191     330      39
 
KasI G'GCGC_C

Cuts at:      0    1504    1560 
   Size:       1504      56
 
MfeI C'AATT_G

Cuts at:      0     562    1560 
   Size:        562     998
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     508    1322    1560 
   Size:        508     814     238

  Fragments arranged by size: 

                 814     508     238
 
MscI TGG'CCA

Cuts at:      0     481     904    1560 
   Size:        481     423     656

  Fragments arranged by size: 

                 656     481     423
 
MspA1I CmG'CkG

Cuts at:      0      35     399    1038    1149    1535    1560 
   Size:         35     364     639     111     386      25

  Fragments arranged by size: 

                 639     386     364     111      35      25
 
NaeI GCC'GGC

Cuts at:      0    1112    1266    1359    1560 
   Size:       1112     154      93     201

  Fragments arranged by size: 

                1112     201     154      93
 
NarI GG'CG_CC

Cuts at:      0    1505    1560 
   Size:       1505      55
 
NcoI C'CATG_G

Cuts at:      0     905    1560 
   Size:        905     655
 
NgoMIV G'CCGG_C

Cuts at:      0    1110    1264    1357    1560 
   Size:       1110     154      93     203

  Fragments arranged by size: 

                1110     203     154      93
 
NspI r_CATG'y

Cuts at:      0     804    1350    1560 
   Size:        804     546     210

  Fragments arranged by size: 

                 804     546     210
 
PasI CC'CwG_GG

Cuts at:      0     720    1134    1560 
   Size:        720     414     426

  Fragments arranged by size: 

                 720     426     414
 
PciI A'CATG_T

Cuts at:      0     800    1560 
   Size:        800     760
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     372     491     889    1560 
   Size:        372     119     398     671

  Fragments arranged by size: 

                 671     398     372     119
 
PpuMI rG'GwC_Cy

Cuts at:      0     468    1045    1560 
   Size:        468     577     515

  Fragments arranged by size: 

                 577     515     468
 
PshAI GACnn'nnGTC

Cuts at:      0    1050    1560 
   Size:       1050     510
 
PspOMI G'GGCC_C

Cuts at:      0     679    1560 
   Size:        679     881
 
PvuII CAG'CTG

Cuts at:      0     399    1038    1149    1560 
   Size:        399     639     111     411

  Fragments arranged by size: 

                 639     411     399     111
 
SacI G_AGCT'C

Cuts at:      0     290    1560 
   Size:        290    1270
 
SacII CC_GC'GG

Cuts at:      0      36    1560 
   Size:         36    1524
 
SalI G'TCGA_C

Cuts at:      0    1519    1560 
   Size:       1519      41
 
SanDI GG'GwC_CC

Cuts at:      0    1045    1560 
   Size:       1045     515
 
SfoI GGC'GCC

Cuts at:      0    1506    1560 
   Size:       1506      54
 
SmlI C'TyrA_G

Cuts at:      0     383    1255    1560 
   Size:        383     872     305

  Fragments arranged by size: 

                 872     383     305
 
SspI AAT'ATT

Cuts at:      0     253    1560 
   Size:        253    1307
 
StuI AGG'CCT

Cuts at:      0    1426    1560 
   Size:       1426     134
 
StyI C'CwwG_G

Cuts at:      0     187     905    1427    1560 
   Size:        187     718     522     133

  Fragments arranged by size: 

                 718     522     187     133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     702    1147    1560 
   Size:        702     445     413

  Fragments arranged by size: 

                 702     445     413
 
TatI w'GTAC_w

Cuts at:      0     592     995    1560 
   Size:        592     403     565

  Fragments arranged by size: 

                 592     565     403
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     154     186    1560 
   Size:        154      32    1374

  Fragments arranged by size: 

                1374     154      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1305    1560 
   Size:       1305     255
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII    AlwNI     ApaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BglI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
   BstAPI   BstEII    BstYI     BtgI     BtsI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII     KasI     MfeI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI    PpuMI
    PshAI   PspOMI    PvuII     SacI    SacII     SalI    SanDI     SfoI
     SmlI     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI
     BsaI    BsaAI    BsaBI    BsaWI    BseYI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MluI     MslI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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