(Linear) (Six-base) MAPSORT of: KIBB5136.seq  Check: 9534  from: 1  to: 798



KIBB5136.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     580     798 
   Size:        580     218
 
AclI AA'CG_TT

Cuts at:      0     645     798 
   Size:        645     153
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     798 
   Size:        268     530
 
AlwNI CAG_nnn'CTG

Cuts at:      0     581     798 
   Size:        581     217
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323     798 
   Size:        323     475
 
Bme1580I G_kGCm'C

Cuts at:      0     317     798 
   Size:        317     481
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     295     314     798 
   Size:        295      19     484

  Fragments arranged by size: 

                 484     295      19
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     626     798 
   Size:        626     172
 
BsaAI yAC'GTr

Cuts at:      0     688     798 
   Size:        688     110
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     578     798 
   Size:        578     220
 
BseYI C'CCAG_C

Cuts at:      0     183     798 
   Size:        183     615
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     798 
   Size:        250     548
 
BsiHKAI G_wGCw'C

Cuts at:      0     507     798 
   Size:        507     291
 
Bsp1286I G_dGCh'C

Cuts at:      0     317     507     798 
   Size:        317     190     291

  Fragments arranged by size: 

                 317     291     190
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     580     798 
   Size:        580     218
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     581     798 
   Size:        581     217
 
BstBI TT'CG_AA

Cuts at:      0     114     798 
   Size:        114     684
 
BtsI GCAGTG_nn'

Cuts at:      0     582     798 
   Size:        582     216
 
EaeI y'GGCC_r

Cuts at:      0     405     550     798 
   Size:        405     145     248

  Fragments arranged by size: 

                 405     248     145
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     324     798 
   Size:        324     474
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     798 
   Size:        268     530
 
EcoO109I rG'GnC_Cy

Cuts at:      0     180     798 
   Size:        180     618
 
EcoRV GAT'ATC

Cuts at:      0      81     798 
   Size:         81     717
 
FspI TGC'GCA

Cuts at:      0     453     798 
   Size:        453     345
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     281     313     623     655     798 
   Size:        281      32     310      32     143

  Fragments arranged by size: 

                 310     281     143      32      32
 
HincII GTy'rAC

Cuts at:      0     621     798 
   Size:        621     177
 
HpaI GTT'AAC

Cuts at:      0     621     798 
   Size:        621     177
 
MfeI C'AATT_G

Cuts at:      0     219     798 
   Size:        219     579
 
MscI TGG'CCA

Cuts at:      0     407     552     798 
   Size:        407     145     246

  Fragments arranged by size: 

                 407     246     145
 
PfoI T'CCnGG_A

Cuts at:      0     362     798 
   Size:        362     436
 
PpuMI rG'GwC_Cy

Cuts at:      0     180     798 
   Size:        180     618
 
PsiI TTA'TAA

Cuts at:      0     748     798 
   Size:        748      50
 
PstI C_TGCA'G

Cuts at:      0     577     798 
   Size:        577     221
 
SanDI GG'GwC_CC

Cuts at:      0     180     798 
   Size:        180     618
 
SfcI C'TryA_G

Cuts at:      0     573     798 
   Size:        573     225
 
SspI AAT'ATT

Cuts at:      0      69     798 
   Size:         69     729
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     284     798 
   Size:        284     514
 
Tth111I GACn'n_nGTC

Cuts at:      0     306     637     798 
   Size:        306     331     161

  Fragments arranged by size: 

                 331     306     161
 

 Enzymes that do cut:

     AarI     AclI     AcuI    AlwNI     BbsI Bme1580I     BmrI     BsaI
    BsaAI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI
    BstBI     BtsI     EaeI     EciI  Eco57MI EcoO109I    EcoRV     FspI
    Hin4I   HincII     HpaI     MfeI     MscI     PfoI    PpuMI     PsiI
     PstI    SanDI     SfcI     SspI    TaqII  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI    EcoRI     FalI
     FseI    FspAI    HaeII  HindIII     KasI     KpnI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI