Restriction map for pF1KB5005
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AlwI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvrII       BamHI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BlnI        BpuAI       BsaBI       
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrGI       Bst1107I    BstAPI      
BstBI       BstZ17I     ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HindIII     HpaI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MroI        MseI        MunI        NciI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcsI          637  AAATTC               [GA]^AATT[CT]
Alw44I        319  GTGCAC               G^TGCAC
AlwNI          83  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1225  CCTAAGG              CC^T[ACGT]AGG
ApaLI         319  GTGCAC               G^TGCAC
ApoI          637  AAATTC               [GA]^AATT[CT]
AspI          678  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         820  CACGTG               CAC^GTG
BfaI         1171  CTAG                 C^TAG
BglI          142  GCCGGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         872  AGATCT               A^GATCT
BlpI          288  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      288  GCTGAGC              GC^T[ACGT]AGC
BsaAI         820  CACGTG               [CT]AC^GT[GA]
BsaWI         713  ACCGGA               [AT]^CCGG[AT]
BsmAI        1244  GAGAC                GTCTC(1/5)
BsmBI        1244  GAGACG               CGTCTC(1/5)
BsmFI        1178  GGGAC                GGGAC(10/14)
BspHI         701  TCATGA               T^CATGA
BsrI          626  ACTGG                ACTGG(1/-1)
BssSI         238  CTCGTG               CACGAG(-5/-1)
BstYI         872  AGATCT               [GA]^GATC[CT]
Bsu36I       1225  CCTAAGG              CC^T[ACGT]AGG
CelII         288  GCTGAGC              GC^T[ACGT]AGC
DraII        1031  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          261  GACCACGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1225  CCTAAGG              CC^T[ACGT]AGG
EcoO109I     1031  AGGACCT              [GA]G^G[ACGT]CC[CT]
FauI         1176  GCGGG                CCCGC(4/6)
HgaI          268  GCGTC                GACGC(5/10)
HincII        601  GTCAAC               GT[CT]^[GA]AC
HindII        601  GTCAAC               GT[CT]^[GA]AC
HinfI         678  GACTC                G^A[ACGT]TC
MaeI         1171  CTAG                 C^TAG
MflI          872  AGATCT               [GA]^GATC[CT]
NcoI          170  CCATGG               C^CATGG
NspI         1016  GCATGT               [GA]CATG^[CT]
PaeR7I        832  CTCGAG               C^TCGAG
PflFI         678  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          678  GACTC                GAGTC(4/5)
PmaCI         820  CACGTG               CAC^GTG
PmlI          820  CACGTG               CAC^GTG
PpuMI        1031  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          701  TCATGA               T^CATGA
SmlI          832  CTCGAG               C^T[CT][GA]AG
StuI          720  AGGCCT               AGG^CCT
TaqI          833  TCGA                 T^CGA
Tsp509I       638  AATT                 ^AATT
Tth111I       678  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       833  TCGA                 T^CGA
XcmI          920  CCAAGGGTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          832  CTCGAG               C^TCGAG
XhoII         872  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AspHI         319  GTGCAC               G[AT]GC[AT]^C
              469  GAGCAC              
AvaI          556  CCCGAG               C^[CT]CG[GA]G
              832  CTCGAG              
AviII         440  TGCGCA               TGC^GCA
             1127  TGCGCA              
BalI          173  TGGCCA               TGG^CCA
             1043  TGGCCA              
BanI          589  GGCGCC               G^G[CT][GA]CC
             1211  GGCGCC              
BanII         108  GAGCCC               G[GA]GC[CT]^C
              235  GGGCTC              
BbeI          589  GGCGCC               GGCGC^C
             1211  GGCGCC              
BpmI          792  CTCCAG               CTGGAG(16/14)
               37  CTGGAG              
BseRI         313  GAGGAG               GAGGAG(10/8)
             1120  GAGGAG              
BsiHKAI       319  GTGCAC               G[AT]GC[AT]^C
              469  GAGCAC              
BsoBI         556  CCCGAG               C^[CT]CG[GA]G
              832  CTCGAG              
BspMI          69  ACCTGC               ACCTGC(4/8)
              947  ACCTGC              
BsrFI         142  GCCGGC               [GA]^CCGG[CT]
              325  GCCGGC              
BssHII        367  GCGCGC               G^CGCGC
              503  GCGCGC              
BstEII        561  GGTCACC              G^GT[ACGT]ACC
              936  GGTGACC             
BstPI         561  GGTCACC              G^GT[ACGT]ACC
              936  GGTGACC             
BstXI         566  CCAAGGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              920  CCAAGGGTGTGG        
Cfr10I        142  GCCGGC               [GA]^CCGG[CT]
              325  GCCGGC              
DpnII         480  GATC                 ^GATC
              873  GATC                
EcoNI          33  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              975  CCTGACTGAGG         
EcoO65I       561  GGTCACC              G^GT[ACGT]ACC
              936  GGTGACC             
FspI          440  TGCGCA               TGC^GCA
             1127  TGCGCA              
KasI          589  GGCGCC               G^GCGCC
             1211  GGCGCC              
MboI          480  GATC                 ^GATC
              873  GATC                
MluNI         173  TGGCCA               TGG^CCA
             1043  TGGCCA              
MscI          173  TGGCCA               TGG^CCA
             1043  TGGCCA              
MspA1I        301  CAGCTG               C[AC]G^C[GT]G
              865  CAGCTG              
NaeI          142  GCCGGC               GCC^GGC
              325  GCCGGC              
NarI          589  GGCGCC               GG^CGCC
             1211  GGCGCC              
NdeII         480  GATC                 ^GATC
              873  GATC                
NgoMIV        142  GCCGGC               G^CCGGC
              325  GCCGGC              
PvuII         301  CAGCTG               CAG^CTG
              865  CAGCTG              
Sau3AI        480  GATC                 ^GATC
              873  GATC                
SfoI          589  GGCGCC               GGC^GCC
             1211  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaII       BbvI        BmyI        BsaHI       BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DsaI        EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        MaeII       MaeIII      MboII       MnlI        
MslI        MspI        MvaI        MvnI        MwoI        
NlaIII      NlaIV       PflMI       PstI        RsaI        
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      TspRI       Van91I      
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