Restriction map for pF1KB5005
 (Linear) (Six-base) MAPSORT of: KIBB5005.seq  Check: 3167  from: 1  to: 1254



KIBB5005.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     889    1254 
   Size:        138     751     365

  Fragments arranged by size: 

                 751     365     138
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     523    1254 
   Size:        523     731
 
AlwNI CAG_nnn'CTG

Cuts at:      0      88    1254 
   Size:         88    1166
 
ApaLI G'TGCA_C

Cuts at:      0     319    1254 
   Size:        319     935
 
ApoI r'AATT_y

Cuts at:      0     637    1254 
   Size:        637     617
 
AvaI C'yCGr_G

Cuts at:      0     556     832    1254 
   Size:        556     276     422

  Fragments arranged by size: 

                 556     422     276
 
BanI G'GyrC_C

Cuts at:      0     589    1211    1254 
   Size:        589     622      43

  Fragments arranged by size: 

                 622     589      43
 
BanII G_rGCy'C

Cuts at:      0     112     239    1254 
   Size:        112     127    1015

  Fragments arranged by size: 

                1015     127     112
 
BbeI G_GCGC'C

Cuts at:      0     593    1215    1254 
   Size:        593     622      39

  Fragments arranged by size: 

                 622     593      39
 
BglI GCCn_nnn'nGGC

Cuts at:      0     148    1254 
   Size:        148    1106
 
BglII A'GATC_T

Cuts at:      0     872    1254 
   Size:        872     382
 
BlpI GC'TnA_GC

Cuts at:      0     289    1254 
   Size:        289     965
 
Bme1580I G_kGCm'C

Cuts at:      0     323    1254 
   Size:        323     931
 
BmgBI CAC'GTC

Cuts at:      0     573    1254 
   Size:        573     681
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     636    1254 
   Size:        636     618
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     777    1254 
   Size:         58     719     477

  Fragments arranged by size: 

                 719     477      58
 
BsaAI yAC'GTr

Cuts at:      0     822    1254 
   Size:        822     432
 
BsaHI Gr'CG_yC

Cuts at:      0     268     590    1212    1254 
   Size:        268     322     622      42

  Fragments arranged by size: 

                 622     322     268      42
 
BsaWI w'CCGG_w

Cuts at:      0     713    1254 
   Size:        713     541
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     775     805    1254 
   Size:        775      30     449

  Fragments arranged by size: 

                 775     449      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328    1135    1254 
   Size:        328     807     119

  Fragments arranged by size: 

                 807     328     119
 
BseYI C'CCAG_C

Cuts at:      0     231     246     387     409     964     969    1254 
   Size:        231      15     141      22     555       5     285

  Fragments arranged by size: 

                 555     285     231     141      22      15       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     323     473    1254 
   Size:        323     150     781

  Fragments arranged by size: 

                 781     323     150
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1238    1254 
   Size:       1238      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     112     239     323     473    1254 
   Size:        112     127      84     150     781

  Fragments arranged by size: 

                 781     150     127     112      84
 
BspHI T'CATG_A

Cuts at:      0     701    1254 
   Size:        701     553
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78     956    1254 
   Size:         78     878     298

  Fragments arranged by size: 

                 878     298      78
 
BsrFI r'CCGG_y

Cuts at:      0     142     325    1254 
   Size:        142     183     929

  Fragments arranged by size: 

                 929     183     142
 
BssHII G'CGCG_C

Cuts at:      0     367     503    1254 
   Size:        367     136     751

  Fragments arranged by size: 

                 751     367     136
 
BssSI C'ACGA_G

Cuts at:      0     238    1254 
   Size:        238    1016
 
BstEII G'GTnAC_C

Cuts at:      0     561     936    1254 
   Size:        561     375     318

  Fragments arranged by size: 

                 561     375     318
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     573     927    1254 
   Size:        573     354     327

  Fragments arranged by size: 

                 573     354     327
 
BstYI r'GATC_y

Cuts at:      0     872    1254 
   Size:        872     382
 
Bsu36I CC'TnA_GG

Cuts at:      0    1226    1254 
   Size:       1226      28
 
BtgI C'CryG_G

Cuts at:      0     170     212     263     362     663     692    1254 
   Size:        170      42      51      99     301      29     562

  Fragments arranged by size: 

                 562     301     170      99      51      42      29
 
BtsI GCAGTG_nn'

Cuts at:      0      92     194     290    1254 
   Size:         92     102      96     964

  Fragments arranged by size: 

                 964     102      96      92
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1050    1085    1254 
   Size:       1050      35     169

  Fragments arranged by size: 

                1050     169      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     267    1254 
   Size:        267     987
 
EaeI y'GGCC_r

Cuts at:      0      50     119     173    1043    1254 
   Size:         50      69      54     870     211

  Fragments arranged by size: 

                 870     211      69      54      50
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     138     777     889    1254 
   Size:         58      80     639     112     365

  Fragments arranged by size: 

                 639     365     112      80      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      37     979    1254 
   Size:         37     942     275

  Fragments arranged by size: 

                 942     275      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1032    1254 
   Size:       1032     222
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     501    1023    1254 
   Size:        501     522     231

  Fragments arranged by size: 

                 522     501     231
 
FspI TGC'GCA

Cuts at:      0     442    1129    1254 
   Size:        442     687     125

  Fragments arranged by size: 

                 687     442     125
 
HaeII r_GCGC'y

Cuts at:      0      26     143     296     593     840    1215    1254 
   Size:         26     117     153     297     247     375      39

  Fragments arranged by size: 

                 375     297     247     153     117      39      26
 
HincII GTy'rAC

Cuts at:      0     603    1254 
   Size:        603     651
 
KasI G'GCGC_C

Cuts at:      0     589    1211    1254 
   Size:        589     622      43

  Fragments arranged by size: 

                 622     589      43
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1055    1254 
   Size:       1055     199
 
MscI TGG'CCA

Cuts at:      0     175    1045    1254 
   Size:        175     870     209

  Fragments arranged by size: 

                 870     209     175
 
MslI CAynn'nnrTG

Cuts at:      0     202     629     706     808    1254 
   Size:        202     427      77     102     446

  Fragments arranged by size: 

                 446     427     202     102      77
 
MspA1I CmG'CkG

Cuts at:      0     303     867    1254 
   Size:        303     564     387

  Fragments arranged by size: 

                 564     387     303
 
NaeI GCC'GGC

Cuts at:      0     144     327    1254 
   Size:        144     183     927

  Fragments arranged by size: 

                 927     183     144
 
NarI GG'CG_CC

Cuts at:      0     590    1212    1254 
   Size:        590     622      42

  Fragments arranged by size: 

                 622     590      42
 
NcoI C'CATG_G

Cuts at:      0     170    1254 
   Size:        170    1084
 
NgoMIV G'CCGG_C

Cuts at:      0     142     325    1254 
   Size:        142     183     929

  Fragments arranged by size: 

                 929     183     142
 
NspI r_CATG'y

Cuts at:      0    1020    1254 
   Size:       1020     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     171     189     651     822    1254 
   Size:        171      18     462     171     432

  Fragments arranged by size: 

                 462     432     171     171      18
 
PfoI T'CCnGG_A

Cuts at:      0      35    1254 
   Size:         35    1219
 
PmlI CAC'GTG

Cuts at:      0     822    1254 
   Size:        822     432
 
PpuMI rG'GwC_Cy

Cuts at:      0    1032    1254 
   Size:       1032     222
 
PstI C_TGCA'G

Cuts at:      0      75     512     953    1254 
   Size:         75     437     441     301

  Fragments arranged by size: 

                 441     437     301      75
 
PvuII CAG'CTG

Cuts at:      0     303     867    1254 
   Size:        303     564     387

  Fragments arranged by size: 

                 564     387     303
 
SfcI C'TryA_G

Cuts at:      0      71     508     949    1254 
   Size:         71     437     441     305

  Fragments arranged by size: 

                 441     437     305      71
 
SfoI GGC'GCC

Cuts at:      0     591    1213    1254 
   Size:        591     622      41

  Fragments arranged by size: 

                 622     591      41
 
SmlI C'TyrA_G

Cuts at:      0     832    1254 
   Size:        832     422
 
StuI AGG'CCT

Cuts at:      0     722    1254 
   Size:        722     532
 
StyI C'CwwG_G

Cuts at:      0     113     170     176     278     566     920    1254 
   Size:        113      57       6     102     288     354     334

  Fragments arranged by size: 

                 354     334     288     113     102      57       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     671     685    1198    1254 
   Size:        671      14     513      56

  Fragments arranged by size: 

                 671     513      56      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     775     807    1254 
   Size:        775      32     447

  Fragments arranged by size: 

                 775     447      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     681    1254 
   Size:        681     573
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     927    1254 
   Size:        927     327
 
XhoI C'TCGA_G

Cuts at:      0     832    1254 
   Size:        832     422
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    ApaLI     ApoI     AvaI     BanI    BanII
     BbeI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrFI   BssHII    BssSI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     DrdI     EaeI  Eco57MI
    EcoNI EcoO109I     FalI     FspI    HaeII   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
    PflMI     PfoI     PmlI    PpuMI     PstI    PvuII     SfcI     SfoI
     SmlI     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrDI    BsrGI   BstAPI
    BstBI  BstZ17I    BtgZI     ClaI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI    EcoRI    EcoRV     FseI    FspAI    Hin4I  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     XbaI     XmaI     XmnI     ZraI
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