Restriction map for pF1KB5004
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AgeI        
AlwI        Aor51HI     AscI        AseI        AsnI        
Asp700I     AvaII       AviII       BalI        BamHI       
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglII       BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BspDI       
BspEI       BspMI       BsrBI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       CelII       ClaI        CpoI        DraI        
DraIII      EagI        EarI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        Ksp632I     KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         325  GACGTC               GACGT^C
AccI           76  GTATAC               GT^[AC][GT]AC
AcsI          958  GAATTT               [GA]^AATT[CT]
AflIII        572  ACATGT               A^C[GA][CT]GT
AhdI          117  GACACTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          958  GAATTT               [GA]^AATT[CT]
AspEI         117  GACACTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          259  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         405  CCTAGG               C^CTAGG
BcgI          692  CGACTCCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          705  GCCTGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          405  CCTAGG               C^CTAGG
BseRI        1134  CTCCTC               GAGGAG(10/8)
BsgI          527  CTGCAC               GTGCAG(16/14)
BsiEI         597  CGACCG               CG[GA][CT]^CG
BsmBI        1131  CGTCTC               CGTCTC(1/5)
BspHI        1071  TCATGA               T^CATGA
BspLU11I      572  ACATGT               A^CATGT
BsrDI         305  GCAATG               GCAATG(2/0)
Bst1107I       76  GTATAC               GTA^TAC
BstXI         554  CCACAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I        76  GTATAC               GTA^TAC
EaeI         1031  TGGCCG               [CT]^GGCC[GA]
Eam1105I      117  GACACTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         937  CCTGAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1075  GATATC               GAT^ATC
HgaI          123  GCGTC                GACGC(5/10)
MaeII         326  ACGT                 A^CGT
MseI          812  TTAA                 T^TAA
NaeI          872  GCCGGC               GCC^GGC
NcoI         1140  CCATGG               C^CATGG
NgoMIV        872  GCCGGC               G^CCGGC
PflFI         259  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         491  CCATGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           53  CTGCAG               CTGCA^G
RcaI         1071  TCATGA               T^CATGA
SfaNI          69  GCATC                GCATC(5/9)
SfcI           53  CTGCAG               C^T[GA][CT]AG
SgrAI         871  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          380  CCCGGG               CCC^GGG
StuI          945  AGGCCT               AGG^CCT
TfiI         1038  GATTC                G^A[AT]TC
Tru9I         812  TTAA                 T^TAA
Tsp509I       959  AATT                 ^AATT
Tth111I       259  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        491  CCATGACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          723  CCACTCGGCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         380  CCCGGG               C^CCGGG
XmaI          380  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

Acc65I        216  GGTACC               G^GTACC
              922  GGTACC              
Alw44I         16  GTGCAC               G^TGCAC
              136  GTGCAC              
AlwNI          56  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              368  CAGTTCCTG           
AocI          706  CCTGAGG              CC^T[ACGT]AGG
              937  CCTGAGG             
ApaI           31  GGGCCC               GGGCC^C
              377  GGGCCC              
ApaLI          16  GTGCAC               G^TGCAC
              136  GTGCAC              
Asp718I       216  GGTACC               G^GTACC
              922  GGTACC              
AvaI          353  CCCGAG               C^[CT]CG[GA]G
              380  CCCGGG              
BanII          31  GGGCCC               G[GA]GC[CT]^C
              377  GGGCCC              
BbeI          713  GGCGCC               GGCGC^C
              875  GGCGCC              
BbsI          106  GAAGAC               GAAGAC(2/6)
             1014  GAAGAC              
BpuAI         106  GAAGAC               GAAGAC(2/6)
             1014  GAAGAC              
BsmAI         115  GAGAC                GTCTC(1/5)
             1132  GTCTC               
BsmI         1092  GAATGC               GAATGC(1/-1)
              644  GCATTC              
BsoBI         353  CCCGAG               C^[CT]CG[GA]G
              380  CCCGGG              
BsrFI         785  ACCGGC               [GA]^CCGG[CT]
              872  GCCGGC              
BstF5I        195  CATCC                GGATG(2/0)
              384  GGATG               
Bsu36I        706  CCTGAGG              CC^T[ACGT]AGG
              937  CCTGAGG             
Cfr10I        785  ACCGGC               [GA]^CCGG[CT]
              872  GCCGGC              
DpnII         414  GATC                 ^GATC
              973  GATC                
DrdI          256  GACGACACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              901  GACAAGATTGTC        
DsaI          198  CCGTGG               C^C[GA][CT]GG
             1140  CCATGG              
Eco81I        706  CCTGAGG              CC^T[ACGT]AGG
              937  CCTGAGG             
EcoT14I       405  CCTAGG               C^C[AT][AT]GG
             1140  CCATGG              
FokI          195  CATCC                GGATG(9/13)
              384  GGATG               
HaeII         713  GGCGCC               [GA]GCGC^[CT]
              875  GGCGCC              
KasI          713  GGCGCC               G^GCGCC
              875  GGCGCC              
KpnI          216  GGTACC               GGTAC^C
              922  GGTACC              
MboI          414  GATC                 ^GATC
              973  GATC                
MboII         106  GAAGA                GAAGA(8/7)
             1014  GAAGA               
MslI          571  CACATGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              790  CACTTCCATG          
NarI          713  GGCGCC               GG^CGCC
              875  GGCGCC              
NdeII         414  GATC                 ^GATC
              973  GATC                
NspI          224  ACATGC               [GA]CATG^[CT]
              572  ACATGT              
Sau3AI        414  GATC                 ^GATC
              973  GATC                
SfoI          713  GGCGCC               GGC^GCC
              875  GGCGCC              
StyI          405  CCTAGG               C^C[AT][AT]GG
             1140  CCATGG              
TaqI          254  TCGA                 T^CGA
              648  TCGA                
Tsp45I        569  GTCAC                ^GT[GC]AC
              899  GTGAC               
TthHB8I       254  TCGA                 T^CGA
              648  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AspHI       
BanI        BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MnlI        MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PleI        RsaI        Sau96I      ScrFI       SspBI       
TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com