Restriction map for
pF1KB5004
(Circular) (Six-base) MAPSORT of: pF1KB5004.seq Check: 1777 from: 1 to: 4259
pF1KB5004 4259 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 437 437
Size: 4259
AccI GT'mk_AC
Cuts at: 185 1297 185
Size: 1112 3147
Acc65I G'GTAC_C
Cuts at: 324 1030 1275 324
Size: 706 245 3308
Fragments arranged by size:
3308 706 245
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2084 2516 3263 3531 1141
Size: 943 432 747 268 1869
Fragments arranged by size:
1869 943 747 432 268
AflIII A'CryG_T
Cuts at: 680 1611 2715 3692 680
Size: 931 1104 977 1247
Fragments arranged by size:
1247 1104 977 931
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AhdI GACnn_n'nnGTC
Cuts at: 230 230
Size: 4259
AlwNI CAG_nnn'CTG
Cuts at: 169 481 1564 3131 169
Size: 312 1083 1567 1297
Fragments arranged by size:
1567 1297 1083 312
ApaI G_GGCC'C
Cuts at: 143 489 143
Size: 346 3913
ApaLI G'TGCA_C
Cuts at: 124 244 3029 124
Size: 120 2785 1354
Fragments arranged by size:
2785 1354 120
ApoI r'AATT_y
Cuts at: 1066 1263 3612 3868 1066
Size: 197 2349 256 1457
Fragments arranged by size:
2349 1457 256 197
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 461 488 1279 461
Size: 27 791 3441
Fragments arranged by size:
3441 791 27
AvrII C'CTAG_G
Cuts at: 513 513
Size: 4259
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 324 821 983 1030 1275 1689 1937 1972 324
Size: 497 162 47 245 414 248 35 2611
Fragments arranged by size:
2611 497 414 248 245 162 47 35
BanII G_rGCy'C
Cuts at: 143 489 1273 2303 143
Size: 346 784 1030 2099
Fragments arranged by size:
2099 1030 784 346
BbeI G_GCGC'C
Cuts at: 825 987 1693 1941 825
Size: 162 706 248 3143
Fragments arranged by size:
3143 706 248 162
BbsI GAAGACnn'nnnn_
Cuts at: 221 1129 221
Size: 908 3351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 789 823 789
Size: 34 4225
BciVI GTATCCnnnnn_n'
Cuts at: 2157 2918 4259 2157
Size: 761 1341 2157
Fragments arranged by size:
2157 1341 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglI GCCn_nnn'nGGC
Cuts at: 819 819
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 1371 1371
Size: 4259
Bme1580I G_kGCm'C
Cuts at: 128 143 248 489 1884 1977 3033 128
Size: 15 105 241 1395 93 1056 1354
Fragments arranged by size:
1395 1354 1056 241 105 93 15
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1552 1887 4031 1471
Size: 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 663 788 151
Size: 512 125 3622
Fragments arranged by size:
3622 512 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2314 2806 3104 1435
Size: 879 492 298 2590
Fragments arranged by size:
2590 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3963 3963
Size: 4259
BsaAI yAC'GTr
Cuts at: 1612 1612
Size: 4259
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 434 822 984 1690 1938 2640 4136 434
Size: 388 162 706 248 702 1496 557
Fragments arranged by size:
1496 706 702 557 388 248 162
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1233 1233
Size: 4259
BseYI C'CCAG_C
Cuts at: 669 1684 2442 3019 3515 669
Size: 1015 758 577 496 1413
Fragments arranged by size:
1413 1015 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 620
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 708 1801 1847 3055 104
Size: 604 1093 46 1208 1308
Fragments arranged by size:
1308 1208 1093 604 46
BsiHKAI G_wGCw'C
Cuts at: 128 248 422 1273 2051 2241 3033 128
Size: 120 174 851 778 190 792 1354
Fragments arranged by size:
1354 851 792 778 190 174 120
BsmI GAATG_Cn'
Cuts at: 752 1206 752
Size: 454 3805
BsmBI CGTCTCn'nnnn_
Cuts at: 1245 1245
Size: 4259
Bsp1286I G_dGCh'C
Cuts at: 128 143 248 422 489 1273 1884 1977 2051
Size: 15 105 174 67 784 611 93 74
Cuts at: 2051 2241 2303 3033 128
Size: 190 62 730 1354
Fragments arranged by size:
1354 784 730 611 190 174 105 93
74 67 62 15
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 1179 3435 1179
Size: 2256 2003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1309 1523 1825 2206 1309
Size: 214 302 381 3362
Fragments arranged by size:
3362 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2551 2605 4089 4254 50
Size: 2501 54 1484 165 55
Fragments arranged by size:
2501 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 420 2171 420
Size: 1751 2508
BsrFI r'CCGG_y
Cuts at: 893 980 2257 2438 2647 893
Size: 87 1277 181 209 2505
Fragments arranged by size:
2505 1277 209 181 87
BsrGI T'GTAC_A
Cuts at: 527 619 860 527
Size: 92 241 3926
Fragments arranged by size:
3926 241 92
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 669 669
Size: 4259
BstYI r'GATC_y
Cuts at: 1 1284 1751 1774 2109 2355 3367 3453 3465
Size: 1283 467 23 335 246 1012 86 12
Cuts at: 3465 3811 1
Size: 346 449
Fragments arranged by size:
1283 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 186 186
Size: 4259
Bsu36I CC'TnA_GG
Cuts at: 815 1046 815
Size: 231 4028
BtgI C'CryG_G
Cuts at: 306 1248 306
Size: 942 3317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2209 2390 2553 2209
Size: 181 163 3915
Fragments arranged by size:
3915 181 163
BtsI GCAGTG_nn'
Cuts at: 170 226 266 1502 170
Size: 56 40 1236 2927
Fragments arranged by size:
2927 1236 56 40
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 370 1015 1965 2823 370
Size: 645 950 858 1806
Fragments arranged by size:
1806 950 858 645
EaeI y'GGCC_r
Cuts at: 1139 1844 2018 2409 2436 3781 1139
Size: 705 174 391 27 1345 1617
Fragments arranged by size:
1617 1345 705 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 2282 2492 2282
Size: 210 4049
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 405 571 2467 2777 2923 4069 405
Size: 166 1896 310 146 1146 595
Fragments arranged by size:
1896 1146 595 310 166 146
EcoICRI GAG'CTC
Cuts at: 1271 1271
Size: 4259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 663 788 1141 2084 2516 3263 3531 151
Size: 512 125 353 943 432 747 268 879
Fragments arranged by size:
943 879 747 512 432 353 268 125
EcoNI CCTnn'n_nnAGG
Cuts at: 1049 1049
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 140 407 485 1398 140
Size: 267 78 913 3001
Fragments arranged by size:
3001 913 267 78
EcoRI G'AATT_C
Cuts at: 1263 1263
Size: 4259
EcoRV GAT'ATC
Cuts at: 1185 1185
Size: 4259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 4259
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 825 987 1693 1941 2963 3942 825
Size: 162 706 248 1022 979 1142
Fragments arranged by size:
1142 1022 979 706 248 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 216 248 594 626 640 672 2101 2131 2133
Size: 32 346 32 14 32 1429 30 2
Cuts at: 2133 2163 216
Size: 30 2312
Fragments arranged by size:
2312 1429 346 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 1298 1298
Size: 4259
KasI G'GCGC_C
Cuts at: 821 983 1689 1937 821
Size: 162 706 248 3143
Fragments arranged by size:
3143 706 248 162
KpnI G_GTAC'C
Cuts at: 328 1034 1279 328
Size: 706 245 3308
Fragments arranged by size:
3308 706 245
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 253 752 2930 3114 253
Size: 499 2178 184 1398
Fragments arranged by size:
2178 1398 499 184
MscI TGG'CCA
Cuts at: 2020 2020
Size: 4259
MslI CAynn'nnrTG
Cuts at: 683 902 2248 2375 683
Size: 219 1346 127 2567
Fragments arranged by size:
2567 1346 219 127
MspA1I CmG'CkG
Cuts at: 1370 1684 2044 3057 3302 1370
Size: 314 360 1013 245 2327
Fragments arranged by size:
2327 1013 360 314 245
NaeI GCC'GGC
Cuts at: 982 2440 982
Size: 1458 2801
NarI GG'CG_CC
Cuts at: 822 984 1690 1938 822
Size: 162 706 248 3143
Fragments arranged by size:
3143 706 248 162
NcoI C'CATG_G
Cuts at: 1248 1248
Size: 4259
NgoMIV G'CCGG_C
Cuts at: 980 2438 980
Size: 1458 2801
NspI r_CATG'y
Cuts at: 336 684 1312 1812 2719 336
Size: 348 628 500 907 1876
Fragments arranged by size:
1876 907 628 500 348
PciI A'CATG_T
Cuts at: 680 2715 680
Size: 2035 2224
PflMI CCAn_nnn'nTGG
Cuts at: 605 605
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PsiI TTA'TAA
Cuts at: 878 878
Size: 4259
PspOMI G'GGCC_C
Cuts at: 139 485 139
Size: 346 3913
PstI C_TGCA'G
Cuts at: 165 1306 1991 165
Size: 1141 685 2433
Fragments arranged by size:
2433 1141 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 1684 2044 1684
Size: 360 3899
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 1273 1273
Size: 4259
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 2282 2492 2282
Size: 210 4049
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 161 1302 1987 2980 3171 33
Size: 128 1141 685 993 191 1121
Fragments arranged by size:
1141 1121 993 685 191 128
SfoI GGC'GCC
Cuts at: 823 985 1691 1939 823
Size: 162 706 248 3143
Fragments arranged by size:
3143 706 248 162
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SgrAI Cr'CCGG_yG
Cuts at: 980 980
Size: 4259
SmaI CCC'GGG
Cuts at: 490 1281 490
Size: 791 3468
SmlI C'TyrA_G
Cuts at: 1414 2329 2821 3083 1414
Size: 915 492 262 2590
Fragments arranged by size:
2590 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
StuI AGG'CCT
Cuts at: 1055 1055
Size: 4259
StyI C'CwwG_G
Cuts at: 513 1248 1393 1747 513
Size: 735 145 354 3025
Fragments arranged by size:
3025 735 354 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1842 1975 2434 2643 1842
Size: 133 459 209 3458
Fragments arranged by size:
3458 459 209 133
TatI w'GTAC_w
Cuts at: 527 619 860 3774 527
Size: 92 241 2914 1012
Fragments arranged by size:
2914 1012 241 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4227
Tth111I GACn'n_nGTC
Cuts at: 370 2056 370
Size: 1686 2573
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 838 1754 838
Size: 916 3343
XmaI C'CCGG_G
Cuts at: 488 1279 488
Size: 791 3468
ZraI GAC'GTC
Cuts at: 435 435
Size: 4259
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI BssHII
BstEII ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI