Restriction map for pF1KB5004
 (Linear) (Six-base) MAPSORT of: KIBB5004.seq  Check: 6170  from: 1  to: 1146



KIBB5004.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AatII G_ACGT'C

Cuts at:      0     329    1146 
   Size:        329     817
 
AccI GT'mk_AC

Cuts at:      0      77    1146 
   Size:         77    1069
 
Acc65I G'GTAC_C

Cuts at:      0     216     922    1146 
   Size:        216     706     224

  Fragments arranged by size: 

                 706     224     216
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1033    1146 
   Size:       1033     113
 
AflIII A'CryG_T

Cuts at:      0     572    1146 
   Size:        572     574
 
AhdI GACnn_n'nnGTC

Cuts at:      0     122    1146 
   Size:        122    1024
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61     373    1146 
   Size:         61     312     773

  Fragments arranged by size: 

                 773     312      61
 
ApaI G_GGCC'C

Cuts at:      0      35     381    1146 
   Size:         35     346     765

  Fragments arranged by size: 

                 765     346      35
 
ApaLI G'TGCA_C

Cuts at:      0      16     136    1146 
   Size:         16     120    1010

  Fragments arranged by size: 

                1010     120      16
 
ApoI r'AATT_y

Cuts at:      0     958    1146 
   Size:        958     188
 
AvaI C'yCGr_G

Cuts at:      0     353     380    1146 
   Size:        353      27     766

  Fragments arranged by size: 

                 766     353      27
 
AvrII C'CTAG_G

Cuts at:      0     405    1146 
   Size:        405     741
 
BanI G'GyrC_C

Cuts at:      0     216     713     875     922    1146 
   Size:        216     497     162      47     224

  Fragments arranged by size: 

                 497     224     216     162      47
 
BanII G_rGCy'C

Cuts at:      0      35     381    1146 
   Size:         35     346     765

  Fragments arranged by size: 

                 765     346      35
 
BbeI G_GCGC'C

Cuts at:      0     717     879    1146 
   Size:        717     162     267

  Fragments arranged by size: 

                 717     267     162
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     113    1021    1146 
   Size:        113     908     125

  Fragments arranged by size: 

                 908     125     113
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     681     715    1146 
   Size:        681      34     431

  Fragments arranged by size: 

                 681     431      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     711    1146 
   Size:        711     435
 
Bme1580I G_kGCm'C

Cuts at:      0      20      35     140     381    1146 
   Size:         20      15     105     241     765

  Fragments arranged by size: 

                 765     241     105      20      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     555     680    1146 
   Size:         43     512     125     466

  Fragments arranged by size: 

                 512     466     125      43
 
BsaHI Gr'CG_yC

Cuts at:      0     326     714     876    1146 
   Size:        326     388     162     270

  Fragments arranged by size: 

                 388     326     270     162
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1125    1146 
   Size:       1125      21
 
BseYI C'CCAG_C

Cuts at:      0     561    1146 
   Size:        561     585
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     512    1146 
   Size:        512     634
 
BsiEI CG_ry'CG

Cuts at:      0     600    1146 
   Size:        600     546
 
BsiHKAI G_wGCw'C

Cuts at:      0      20     140     314    1146 
   Size:         20     120     174     832

  Fragments arranged by size: 

                 832     174     120      20
 
BsmI GAATG_Cn'

Cuts at:      0     644    1098    1146 
   Size:        644     454      48

  Fragments arranged by size: 

                 644     454      48
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1137    1146 
   Size:       1137       9
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      35     140     314     381    1146 
   Size:         20      15     105     174      67     765

  Fragments arranged by size: 

                 765     174     105      67      20      15
 
BspHI T'CATG_A

Cuts at:      0    1071    1146 
   Size:       1071      75
 
BsrDI GCAATG_nn'

Cuts at:      0     312    1146 
   Size:        312     834
 
BsrFI r'CCGG_y

Cuts at:      0     785     872    1146 
   Size:        785      87     274

  Fragments arranged by size: 

                 785     274      87
 
BsrGI T'GTAC_A

Cuts at:      0     419     511     752    1146 
   Size:        419      92     241     394

  Fragments arranged by size: 

                 419     394     241      92
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     561    1146 
   Size:        561     585
 
BstZ17I GTA'TAC

Cuts at:      0      78    1146 
   Size:         78    1068
 
Bsu36I CC'TnA_GG

Cuts at:      0     707     938    1146 
   Size:        707     231     208

  Fragments arranged by size: 

                 707     231     208
 
BtgI C'CryG_G

Cuts at:      0     198    1140    1146 
   Size:        198     942       6

  Fragments arranged by size: 

                 942     198       6
 
BtsI GCAGTG_nn'

Cuts at:      0      62     118     158    1146 
   Size:         62      56      40     988

  Fragments arranged by size: 

                 988      62      56      40
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     262     907    1146 
   Size:        262     645     239

  Fragments arranged by size: 

                 645     262     239
 
EaeI y'GGCC_r

Cuts at:      0    1031    1146 
   Size:       1031     115
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     297     463    1146 
   Size:        297     166     683

  Fragments arranged by size: 

                 683     297     166
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     555     680    1033    1146 
   Size:         43     512     125     353     113

  Fragments arranged by size: 

                 512     353     125     113      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     941    1146 
   Size:        941     205
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32     299     377    1146 
   Size:         32     267      78     769

  Fragments arranged by size: 

                 769     267      78      32
 
EcoRV GAT'ATC

Cuts at:      0    1077    1146 
   Size:       1077      69
 
HaeII r_GCGC'y

Cuts at:      0     717     879    1146 
   Size:        717     162     267

  Fragments arranged by size: 

                 717     267     162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     108     140     486     518     532     564    1146 
   Size:        108      32     346      32      14      32     582

  Fragments arranged by size: 

                 582     346     108      32      32      32      14
 
KasI G'GCGC_C

Cuts at:      0     713     875    1146 
   Size:        713     162     271

  Fragments arranged by size: 

                 713     271     162
 
KpnI G_GTAC'C

Cuts at:      0     220     926    1146 
   Size:        220     706     220

  Fragments arranged by size: 

                 706     220     220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     644    1146 
   Size:        145     499     502

  Fragments arranged by size: 

                 502     499     145
 
MslI CAynn'nnrTG

Cuts at:      0     575     794    1146 
   Size:        575     219     352

  Fragments arranged by size: 

                 575     352     219
 
NaeI GCC'GGC

Cuts at:      0     874    1146 
   Size:        874     272
 
NarI GG'CG_CC

Cuts at:      0     714     876    1146 
   Size:        714     162     270

  Fragments arranged by size: 

                 714     270     162
 
NcoI C'CATG_G

Cuts at:      0    1140    1146 
   Size:       1140       6
 
NgoMIV G'CCGG_C

Cuts at:      0     872    1146 
   Size:        872     274
 
NspI r_CATG'y

Cuts at:      0     228     576    1146 
   Size:        228     348     570

  Fragments arranged by size: 

                 570     348     228
 
PciI A'CATG_T

Cuts at:      0     572    1146 
   Size:        572     574
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     497    1146 
   Size:        497     649
 
PsiI TTA'TAA

Cuts at:      0     770    1146 
   Size:        770     376
 
PspOMI G'GGCC_C

Cuts at:      0      31     377    1146 
   Size:         31     346     769

  Fragments arranged by size: 

                 769     346      31
 
PstI C_TGCA'G

Cuts at:      0      57    1146 
   Size:         57    1089
 
SfcI C'TryA_G

Cuts at:      0      53    1146 
   Size:         53    1093
 
SfoI GGC'GCC

Cuts at:      0     715     877    1146 
   Size:        715     162     269

  Fragments arranged by size: 

                 715     269     162
 
SgrAI Cr'CCGG_yG

Cuts at:      0     872    1146 
   Size:        872     274
 
SmaI CCC'GGG

Cuts at:      0     382    1146 
   Size:        382     764
 
StuI AGG'CCT

Cuts at:      0     947    1146 
   Size:        947     199
 
StyI C'CwwG_G

Cuts at:      0     405    1140    1146 
   Size:        405     735       6

  Fragments arranged by size: 

                 735     405       6
 
TatI w'GTAC_w

Cuts at:      0     419     511     752    1146 
   Size:        419      92     241     394

  Fragments arranged by size: 

                 419     394     241      92
 
Tth111I GACn'n_nGTC

Cuts at:      0     262    1146 
   Size:        262     884
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     730    1146 
   Size:        730     416
 
XmaI C'CCGG_G

Cuts at:      0     380    1146 
   Size:        380     766
 
ZraI GAC'GTC

Cuts at:      0     327    1146 
   Size:        327     819
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     AhdI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
     BcgI     BglI Bme1580I     BpmI    BsaHI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrDI    BsrFI
    BsrGI    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I     KasI
     KpnI     MmeI     MslI     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PsiI   PspOMI     PstI     SfcI     SfoI    SgrAI
     SmaI     StuI     StyI     TatI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AleI     AlfI     AloI
     AscI     AseI    AsiSI     BaeI    BamHI    BbvCI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI    BspEI
    BspMI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI  EcoICRI
    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     MfeI     MluI     MscI   MspA1I     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TstI     XbaI     XhoI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com