Restriction map for pF1KB5000
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO109I    EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HindIII     KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         143  CGCG                 CG^CG
AcyI          320  GACGCC               G[GA]^CG[CT]C
AocI          226  CCTAAGG              CC^T[ACGT]AGG
AvaII          72  GGTCC                G^G[AT]CC
BpmI          592  CTCCAG               CTGGAG(16/14)
BsaHI         320  GACGCC               G[GA]^CG[CT]C
BseRI         307  GAGGAG               GAGGAG(10/8)
BssHII        142  GCGCGC               G^CGCGC
BstF5I         18  CATCC                GGATG(2/0)
BstNI          21  CCAGG                CC^[AT]GG
BstUI         143  CGCG                 CG^CG
BstXI         543  CCACACTATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        226  CCTAAGG              CC^T[ACGT]AGG
DraIII        441  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          207  TGGCCG               [CT]^GGCC[GA]
Eco81I        226  CCTAAGG              CC^T[ACGT]AGG
EcoNI         519  CCTTGGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         348  GAATTC               G^AATTC
EcoRII         21  CCAGG                ^CC[AT]GG
EcoT14I       519  CCTTGG               C^C[AT][AT]GG
FauI          119  CCCGC                CCCGC(4/6)
FokI           18  CATCC                GGATG(9/13)
HgaI          320  GACGC                GACGC(5/10)
Hin1I         320  GACGCC               G[GA]^CG[CT]C
HincII        265  GTTAAC               GT[CT]^[GA]AC
HindII        265  GTTAAC               GT[CT]^[GA]AC
HpaI          265  GTTAAC               GTT^AAC
MaeII         176  ACGT                 A^CGT
MseI          266  TTAA                 T^TAA
MslI          256  CATCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         75  CCGCTG               C[AC]G^C[GT]G
MvaI           21  CCAGG                CC^[AT]GG
MvnI          143  CGCG                 CG^CG
PleI          341  GAGTC                GAGTC(4/5)
PstI          497  CTGCAG               CTGCA^G
SfaNI         480  GATGC                GCATC(5/9)
SfcI          497  CTGCAG               C^T[GA][CT]AG
StyI          519  CCTTGG               C^C[AT][AT]GG
TaqI          285  TCGA                 T^CGA
Tru9I         266  TTAA                 T^TAA
TthHB8I       285  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcsI          348  GAATTC               [GA]^AATT[CT]
              382  AAATTT              
AfaI           15  GTAC                 GT^AC
               87  GTAC                
AlwI          406  GATCC                GGATC(4/5)
              449  GGATC               
ApoI          348  GAATTC               [GA]^AATT[CT]
              382  AAATTT              
BanII         113  GGGCTC               G[GA]GC[CT]^C
              332  GGGCTC              
BbvI          568  GCAGC                GCAGC(8/12)
               77  GCTGC               
BcnI          128  CCCGG                CC^[GC]GG
              564  CCGGG               
BglI          146  GCCGACTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              200  GCCGTGGTGGC         
BmyI          113  GGGCTC               G[AGT]GC[ACT]^C
              332  GGGCTC              
BsiYI         324  CCACTGTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              519  CCTTGGAAAGG         
BslI          324  CCACTGTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              519  CCTTGGAAAGG         
Bsp1286I      113  GGGCTC               G[AGT]GC[ACT]^C
              332  GGGCTC              
Cfr13I         72  GGTCC                G^G[ACGT]CC
              528  GGGCC               
DpnII         406  GATC                 ^GATC
              450  GATC                
DsaI          201  CCGTGG               C^C[GA][CT]GG
              513  CCGTGG              
Fnu4HI         77  GCTGC                GC^[ACGT]GC
              568  GCAGC               
HapII         129  CCGG                 C^CGG
              564  CCGG                
HpaII         129  CCGG                 C^CGG
              564  CCGG                
HphI          364  GGTGA                GGTGA(8/7)
              452  TCACC               
ItaI           77  GCTGC                GC^[ACGT]GC
              568  GCAGC               
MaeIII        173  GTTAC                ^GT[ACGT]AC
              605  GTTAC               
MboI          406  GATC                 ^GATC
              450  GATC                
MspI          129  CCGG                 C^CGG
              564  CCGG                
NciI          128  CCCGG                CC^[GC]GG
              564  CCGGG               
NdeII         406  GATC                 ^GATC
              450  GATC                
NlaIII         54  CATG                 CATG^
              259  CATG                
NlaIV          71  GGGTCC               GG[ACGT]^[ACGT]CC
              114  GGCTCC              
RsaI           15  GTAC                 GT^AC
               87  GTAC                
Sau3AI        406  GATC                 ^GATC
              450  GATC                
Sau96I         72  GGTCC                G^G[ACGT]CC
              528  GGGCC               
TfiI          189  GATTC                G^A[AT]TC
              370  GATTC               
TseI           77  GCTGC                G^C[AT]GC
              568  GCAGC               
Tsp509I       349  AATT                 ^AATT
              383  AATT                
TspRI         325  CACTG                CA[GC]TG[ACGT][ACGT]^
              445  CAGTG               
XcmI          540  CCACCACACTATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              543  CCACACTATTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        AluI        BsaJI       BsrI        BssKI       
Cac8I       CfoI        DdeI        HaeIII      HhaI        
HinP1I      HinfI       MboII       MnlI        MwoI        
ScrFI       
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