Restriction map for pF1KE3971
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BbvCI       BcgI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT22I     FseI        FspI        HgaI        HphI        
Ksp632I     KspI        MluI        MluNI       MroI        
MscI        MvnI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        430  GGTACC               G^GTACC
AccI          545  GTAGAC               GT^[AC][GT]AC
AcsI         1066  GAATTT               [GA]^AATT[CT]
AcyI           49  GGCGCC               G[GA]^CG[CT]C
AflII         850  CTTAAG               C^TTAAG
AlwNI        1250  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1005  CCTAAGG              CC^T[ACGT]AGG
ApoI         1066  GAATTT               [GA]^AATT[CT]
Asp718I       430  GGTACC               G^GTACC
AspHI         219  GAGCTC               G[AT]GC[AT]^C
BbeI           49  GGCGCC               GGCGC^C
BciVI          82  GTATCC               GTATCC(6/5)
BcnI          934  CCGGG                CC^[GC]GG
BfrI          850  CTTAAG               C^TTAAG
BglI           41  GCCCAGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         139  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          49  GGCGCC               G[GA]^CG[CT]C
BsaI         1274  GAGACC               GGTCTC(1/5)
BsaWI          92  TCCGGT               [AT]^CCGG[AT]
BsiHKAI       219  GAGCTC               G[AT]GC[AT]^C
BspDI         163  ATCGAT               AT^CGAT
BstEII       1180  GGTAACC              G^GT[ACGT]ACC
BstPI        1180  GGTAACC              G^GT[ACGT]ACC
Bsu36I       1005  CCTAAGG              CC^T[ACGT]AGG
CfoI           50  GCGC                 GCG^C
ClaI          163  ATCGAT               AT^CGAT
DsaI           62  CCGTGG               C^C[GA][CT]GG
Eco81I       1005  CCTAAGG              CC^T[ACGT]AGG
EcoNI         846  CCTCCTTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1180  GGTAACC              G^GT[ACGT]ACC
HaeII          49  GGCGCC               [GA]GCGC^[CT]
HhaI           50  GCGC                 GCG^C
Hin1I          49  GGCGCC               G[GA]^CG[CT]C
HinP1I         50  GCGC                 G^CGC
HpaI         1000  GTTAAC               GTT^AAC
KasI           49  GGCGCC               G^GCGCC
KpnI          430  GGTACC               GGTAC^C
MamI          139  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          640  CAATTG               C^AATTG
MslI         1264  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          640  CAATTG               C^AATTG
NarI           49  GGCGCC               GG^CGCC
NciI          934  CCGGG                CC^[GC]GG
PflMI         273  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          424  GACTC                GAGTC(4/5)
PpuMI         663  GGGACCT              [GA]G^G[AT]CC[CT]
SacI          219  GAGCTC               GAGCT^C
ScaI          599  AGTACT               AGT^ACT
SfoI           49  GGCGCC               GGC^GCC
StuI          867  AGGCCT               AGG^CCT
Tsp45I        719  GTCAC                ^GT[GC]AC
TspRI        1284  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        273  CCACAAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

ApaI           39  GGGCCC               GGGCC^C
               68  GGGCCC              
Asp700I       114  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              535  GAAGCCCTTC          
BanI           49  GGCGCC               G^G[CT][GA]CC
              430  GGTACC              
BclI          360  TGATCA               T^GATCA
              792  TGATCA              
BseRI         844  CTCCTC               GAGGAG(10/8)
             1055  GAGGAG              
BsmFI         663  GGGAC                GGGAC(10/14)
             1010  GGGAC               
BspHI         672  TCATGA               T^CATGA
              861  TCATGA              
BspMI         666  ACCTGC               ACCTGC(4/8)
              942  ACCTGC              
BstXI         596  CCAAGTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              816  CCAAGGTGTTGG        
EcoT14I       816  CCAAGG               C^C[AT][AT]GG
              870  CCTTGG              
FauI            7  GCGGG                CCCGC(4/6)
               77  GCGGG               
FbaI          360  TGATCA               T^GATCA
              792  TGATCA              
HincII        991  GTCAAC               GT[CT]^[GA]AC
             1000  GTTAAC              
HindII        991  GTCAAC               GT[CT]^[GA]AC
             1000  GTTAAC              
HindIII       128  AAGCTT               A^AGCTT
              679  AAGCTT              
MspA1I        373  CAGCTG               C[AC]G^C[GT]G
              388  CAGCTG              
PstI          391  CTGCAG               CTGCA^G
              741  CTGCAG              
PvuII         373  CAGCTG               CAG^CTG
              388  CAGCTG              
RcaI          672  TCATGA               T^CATGA
              861  TCATGA              
SfcI          391  CTGCAG               C^T[GA][CT]AG
              741  CTGCAG              
SmlI          460  CTTGAG               C^T[CT][GA]AG
              850  CTTAAG              
StyI          816  CCAAGG               C^C[AT][AT]GG
              870  CCTTGG              
TaqI          122  TCGA                 T^CGA
              164  TCGA                
TfiI          799  GATTC                G^A[AT]TC
             1099  GATTC               
TthHB8I       122  TCGA                 T^CGA
              164  TCGA                
XmnI          114  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              535  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BsgI        BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       Tru9I       
TseI        Tsp509I     XhoII       
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