Restriction map for pF1KB4894
 (Linear) (Six-base) MAPSORT of: KIBB4894.seq  Check: 4931  from: 1  to: 1221



KIBB4894.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AflIII A'CryG_T

Cuts at:      0     231     634     828    1221 
   Size:        231     403     194     393

  Fragments arranged by size: 

                 403     393     231     194
 
AleI CACnn'nnGTG

Cuts at:      0     374    1221 
   Size:        374     847
 
ApaI G_GGCC'C

Cuts at:      0     748    1221 
   Size:        748     473
 
ApaLI G'TGCA_C

Cuts at:      0     169    1221 
   Size:        169    1052
 
AvaI C'yCGr_G

Cuts at:      0     198     506     650     686     748    1221 
   Size:        198     308     144      36      62     473

  Fragments arranged by size: 

                 473     308     198     144      62      36
 
BamHI G'GATC_C

Cuts at:      0     785    1173    1221 
   Size:        785     388      48

  Fragments arranged by size: 

                 785     388      48
 
BanI G'GyrC_C

Cuts at:      0     133     267     305     489     691     907    1221 
   Size:        133     134      38     184     202     216     314

  Fragments arranged by size: 

                 314     216     202     184     134     133      38
 
BanII G_rGCy'C

Cuts at:      0      65     199     507     748    1072    1221 
   Size:         65     134     308     241     324     149

  Fragments arranged by size: 

                 324     308     241     149     134      65
 
BbeI G_GCGC'C

Cuts at:      0     271     309     695    1221 
   Size:        271      38     386     526

  Fragments arranged by size: 

                 526     386     271      38
 
BglI GCCn_nnn'nGGC

Cuts at:      0     303     487     731    1002    1109    1221 
   Size:        303     184     244     271     107     112

  Fragments arranged by size: 

                 303     271     244     184     112     107
 
Bme1580I G_kGCm'C

Cuts at:      0     138     173     748     780    1221 
   Size:        138      35     575      32     441

  Fragments arranged by size: 

                 575     441     138      35      32
 
BmgBI CAC'GTC

Cuts at:      0     637    1221 
   Size:        637     584
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     787    1221 
   Size:        787     434
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     907     927     999    1221 
   Size:        127     780      20      72     222

  Fragments arranged by size: 

                 780     222     127      72      20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     829    1221 
   Size:        479     350     392

  Fragments arranged by size: 

                 479     392     350
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     277    1221 
   Size:        277     944
 
BsaAI yAC'GTr

Cuts at:      0     168     831    1221 
   Size:        168     663     390

  Fragments arranged by size: 

                 663     390     168
 
BsaHI Gr'CG_yC

Cuts at:      0      56     268     306     692    1221 
   Size:         56     212      38     386     529

  Fragments arranged by size: 

                 529     386     212      56      38
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     282     312     613     643    1221 
   Size:        282      30     301      30     578

  Fragments arranged by size: 

                 578     301     282      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1004    1063    1183    1221 
   Size:       1004      59     120      38

  Fragments arranged by size: 

                1004     120      59      38
 
BseYI C'CCAG_C

Cuts at:      0     420     984    1221 
   Size:        420     564     237

  Fragments arranged by size: 

                 564     420     237
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     388     685     796    1221 
   Size:         52     336     297     111     425

  Fragments arranged by size: 

                 425     336     297     111      52
 
BsiEI CG_ry'CG

Cuts at:      0      50     573    1221 
   Size:         50     523     648

  Fragments arranged by size: 

                 648     523      50
 
BsiHKAI G_wGCw'C

Cuts at:      0     173     205    1221 
   Size:        173      32    1016

  Fragments arranged by size: 

                1016     173      32
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     138     173     199     205     507     748     780 
   Size:         65      73      35      26       6     302     241      32

Cuts at:    780    1072    1221 
   Size:        292     149

  Fragments arranged by size: 

                 302     292     241     149      73      65      35      32
                  26       6
 
BsrBI CCG'CTC

Cuts at:      0    1002    1221 
   Size:       1002     219
 
BsrFI r'CCGG_y

Cuts at:      0     407     738    1221 
   Size:        407     331     483

  Fragments arranged by size: 

                 483     407     331
 
BssHII G'CGCG_C

Cuts at:      0     147     149    1221 
   Size:        147       2    1072

  Fragments arranged by size: 

                1072     147       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1217    1221 
   Size:       1217       4
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     264    1221 
   Size:        264     957
 
BstYI r'GATC_y

Cuts at:      0     785    1173    1221 
   Size:        785     388      48

  Fragments arranged by size: 

                 785     388      48
 
Bsu36I CC'TnA_GG

Cuts at:      0     139    1007    1221 
   Size:        139     868     214

  Fragments arranged by size: 

                 868     214     139
 
BtgI C'CryG_G

Cuts at:      0      50      86     540     659    1221 
   Size:         50      36     454     119     562

  Fragments arranged by size: 

                 562     454     119      50      36
 
BtsI GCAGTG_nn'

Cuts at:      0     719     778     813    1221 
   Size:        719      59      35     408

  Fragments arranged by size: 

                 719     408      59      35
 
EaeI y'GGCC_r

Cuts at:      0      78     560     719    1221 
   Size:         78     482     159     502

  Fragments arranged by size: 

                 502     482     159      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     907     927     999    1221 
   Size:        127     780      20      72     222

  Fragments arranged by size: 

                 780     222     127      72      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     417     745     799     989    1089    1221 
   Size:        417     328      54     190     100     132

  Fragments arranged by size: 

                 417     328     190     132     100      54
 
EcoRV GAT'ATC

Cuts at:      0     861    1221 
   Size:        861     360
 
HaeII r_GCGC'y

Cuts at:      0     122     271     309     695     763    1221 
   Size:        122     149      38     386      68     458

  Fragments arranged by size: 

                 458     386     149     122      68      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     282     314     584     613     616     645    1221 
   Size:        282      32     270      29       3      29     576

  Fragments arranged by size: 

                 576     282     270      32      29      29       3
 
KasI G'GCGC_C

Cuts at:      0     267     305     691    1221 
   Size:        267      38     386     530

  Fragments arranged by size: 

                 530     386     267      38
 
MluI A'CGCG_T

Cuts at:      0     231    1221 
   Size:        231     990
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      17    1221 
   Size:         17    1204
 
MscI TGG'CCA

Cuts at:      0     721    1221 
   Size:        721     500
 
MslI CAynn'nnrTG

Cuts at:      0     374    1221 
   Size:        374     847
 
MspA1I CmG'CkG

Cuts at:      0      52     333     756    1221 
   Size:         52     281     423     465

  Fragments arranged by size: 

                 465     423     281      52
 
NarI GG'CG_CC

Cuts at:      0     268     306     692    1221 
   Size:        268      38     386     529

  Fragments arranged by size: 

                 529     386     268      38
 
PasI CC'CwG_GG

Cuts at:      0     413    1221 
   Size:        413     808
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      87    1221 
   Size:         87    1134
 
PmlI CAC'GTG

Cuts at:      0     168     831    1221 
   Size:        168     663     390

  Fragments arranged by size: 

                 663     390     168
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     304     336    1221 
   Size:        304      32     885

  Fragments arranged by size: 

                 885     304      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     417     799    1089    1221 
   Size:        417     382     290     132

  Fragments arranged by size: 

                 417     382     290     132
 
PspOMI G'GGCC_C

Cuts at:      0     744    1221 
   Size:        744     477
 
PspXI vC'TCGA_Gb

Cuts at:      0     198    1221 
   Size:        198    1023
 
PvuII CAG'CTG

Cuts at:      0     333    1221 
   Size:        333     888
 
SacII CC_GC'GG

Cuts at:      0      53    1221 
   Size:         53    1168
 
SanDI GG'GwC_CC

Cuts at:      0     417    1221 
   Size:        417     804
 
ScaI AGT'ACT

Cuts at:      0    1024    1221 
   Size:       1024     197
 
SfcI C'TryA_G

Cuts at:      0     222    1221 
   Size:        222     999
 
SfoI GGC'GCC

Cuts at:      0     269     307     693    1221 
   Size:        269      38     386     528

  Fragments arranged by size: 

                 528     386     269      38
 
SmaI CCC'GGG

Cuts at:      0     750    1221 
   Size:        750     471
 
SmlI C'TyrA_G

Cuts at:      0     198     494     844    1221 
   Size:        198     296     350     377

  Fragments arranged by size: 

                 377     350     296     198
 
StyI C'CwwG_G

Cuts at:      0    1058    1221 
   Size:       1058     163
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     121     193    1221 
   Size:        121      72    1028

  Fragments arranged by size: 

                1028     121      72
 
TatI w'GTAC_w

Cuts at:      0    1022    1160    1221 
   Size:       1022     138      61

  Fragments arranged by size: 

                1022     138      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     770     802    1221 
   Size:        770      32     419

  Fragments arranged by size: 

                 770     419      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     180    1221 
   Size:        180    1041
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     882     969    1221 
   Size:        882      87     252

  Fragments arranged by size: 

                 882     252      87
 
XhoI C'TCGA_G

Cuts at:      0     198    1221 
   Size:        198    1023
 
XmaI C'CCGG_G

Cuts at:      0     748    1221 
   Size:        748     473
 
XmnI GAAnn'nnTTC

Cuts at:      0      22     442    1221 
   Size:         22     420     779

  Fragments arranged by size: 

                 779     420      22
 

 Enzymes that do cut:

   AflIII     AleI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII
     BbeI     BglI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BsrBI    BsrFI   BssHII   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I
     KasI     MluI     MmeI     MscI     MslI   MspA1I     NarI     PasI
    PflMI     PmlI     PpiI    PpuMI   PspOMI    PspXI    PvuII    SacII
    SanDI     ScaI     SfcI     SfoI     SmaI     SmlI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI     BmtI     BplI   Bpu10I    BsaBI    BsaWI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI    PshAI     PsiI
     PsrI     PstI     PvuI    RsrII     SacI     SalI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     XbaI     ZraI
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